NRT32_ARATH
ID NRT32_ARATH Reviewed; 209 AA.
AC Q9SB67; A0A1P8B438; B3H755; F4JR12; Q1G2Z1;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=High-affinity nitrate transporter 3.2 {ECO:0000303|PubMed:16415212};
DE Flags: Precursor;
GN Name=NRT3.2 {ECO:0000303|PubMed:16415212};
GN OrderedLocusNames=At4g24715 {ECO:0000312|Araport:AT4G24715};
GN ORFNames=F22K18.80 {ECO:0000312|EMBL:CAA22991.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY NITRATE.
RX PubMed=16415212; DOI=10.1104/pp.105.074385;
RA Okamoto M., Kumar A., Li W., Wang Y., Siddiqi M.Y., Crawford N.M.,
RA Glass A.D.;
RT "High-affinity nitrate transport in roots of Arabidopsis depends on
RT expression of the NAR2-like gene AtNRT3.1.";
RL Plant Physiol. 140:1036-1046(2006).
CC -!- FUNCTION: Acts as a dual component transporter with NTR2.1. Required
CC for high-affinity nitrate transport (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:16415212}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Bearly detected in roots and shoots.
CC {ECO:0000269|PubMed:16415212}.
CC -!- INDUCTION: Not regulated by nitrate. {ECO:0000269|PubMed:16415212}.
CC -!- SIMILARITY: Belongs to the NAR2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABF59166.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=ANM66321.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL035356; CAA22991.1; -; Genomic_DNA.
DR EMBL; AL161562; CAB79382.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66321.1; ALT_INIT; Genomic_DNA.
DR EMBL; DQ492237; ABF59166.1; ALT_INIT; mRNA.
DR PIR; T05562; T05562.
DR RefSeq; NP_001190826.1; NM_001203897.1.
DR AlphaFoldDB; Q9SB67; -.
DR BioGRID; 13864; 1.
DR PRIDE; Q9SB67; -.
DR ProteomicsDB; 249051; -.
DR DNASU; 828575; -.
DR EnsemblPlants; AT4G24715.1; AT4G24715.1; AT4G24715.
DR GeneID; 28720181; -.
DR Gramene; AT4G24715.1; AT4G24715.1; AT4G24715.
DR KEGG; ath:AT4G24715; -.
DR Araport; AT4G24715; -.
DR TAIR; locus:2121969; AT4G24730.
DR InParanoid; Q9SB67; -.
DR OrthoDB; 1249242at2759; -.
DR PRO; PR:Q9SB67; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SB67; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0010167; P:response to nitrate; IBA:GO_Central.
DR InterPro; IPR016605; Transptr_NO3_Nar2.
DR PANTHER; PTHR34806; PTHR34806; 1.
DR Pfam; PF16974; NAR2; 1.
DR PIRSF; PIRSF012939; Transpt_NO3_Nar2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..209
FT /note="High-affinity nitrate transporter 3.2"
FT /id="PRO_0000400106"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 209 AA; 23304 MW; 6507DBE2F2195AC7 CRC64;
MAIHTLLFVS LLIFSLIESS SGGKKDRLFT DLQNSIEVTA KPVKDSGVLE AGKDMVTITW
KLKSSSAKVD TDTAFKTIQV KLCYAPISQV DRPWRKTDNK LFKDRSCPHE IVSKAYDKTP
QSLDWTIGLD IPTGTYFVRA YGIDGDGHEV AYGQSTDEGR TTNLFSVHAI SGHHVGLDIA
STFFSVFSVV SLFVFFVMEK RKAKLEQRE
