NRTA_NOSS1
ID NRTA_NOSS1 Reviewed; 440 AA.
AC Q44292; O06469;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 3.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Nitrate/nitrite binding protein NrtA {ECO:0000305};
DE Flags: Precursor;
GN Name=nrtA; OrderedLocusNames=alr0608;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8990301; DOI=10.1128/jb.179.2.477-486.1997;
RA Frias J.E., Flores E., Herrero A.;
RT "Nitrate assimilation gene cluster from the heterocyst-forming
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL J. Bacteriol. 179:477-486(1997).
RN [2]
RP SEQUENCE REVISION.
RA Frias J.E.;
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
RX PubMed=8982006; DOI=10.1128/jb.179.1.258-266.1997;
RA Cai Y., Wolk C.P.;
RT "Nitrogen deprivation of Anabaena sp. strain PCC 7120 elicits rapid
RT activation of a gene cluster that is essential for uptake and utilization
RT of nitrate.";
RL J. Bacteriol. 179:258-266(1997).
CC -!- FUNCTION: Part of the ABC transporter complex NrtABCD involved in
CC nitrate uptake. The complex is probably also involved in nitrite
CC transport. NrtA is the substrate-binding protein.
CC {ECO:0000250|UniProtKB:P38043}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NrtC and
CC NrtD), two transmembrane proteins (NrtB) and a solute-binding protein
CC (NrtA). {ECO:0000250|UniProtKB:P38043}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P38043}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38043}; Periplasmic side
CC {ECO:0000250|UniProtKB:P38043}.
CC -!- SIMILARITY: Belongs to the CmpA/NrtA family. {ECO:0000305}.
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DR EMBL; X99709; CAA68041.2; -; Genomic_DNA.
DR EMBL; BA000019; BAB72566.1; -; Genomic_DNA.
DR EMBL; U61496; AAC46075.1; -; Genomic_DNA.
DR PIR; AG1882; AG1882.
DR RefSeq; WP_010994784.1; NZ_RSCN01000009.1.
DR AlphaFoldDB; Q44292; -.
DR SMR; Q44292; -.
DR STRING; 103690.17129954; -.
DR TCDB; 3.A.1.16.4; the atp-binding cassette (abc) superfamily.
DR EnsemblBacteria; BAB72566; BAB72566; BAB72566.
DR KEGG; ana:alr0608; -.
DR eggNOG; COG0715; Bacteria.
DR OMA; GPKKDMA; -.
DR OrthoDB; 1832232at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd13553; PBP2_NrtA_CpmA_like; 1.
DR InterPro; IPR044527; NrtA/CpmA_ABC-bd_dom.
DR InterPro; IPR006311; TAT_signal.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Ion transport; Lipoprotein; Membrane;
KW Nitrate assimilation; Palmitate; Reference proteome; Signal; Transport.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..440
FT /note="Nitrate/nitrite binding protein NrtA"
FT /id="PRO_0000057955"
FT REGION 30..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 100
FT /ligand="nitrate"
FT /ligand_id="ChEBI:CHEBI:17632"
FT /evidence="ECO:0000250|UniProtKB:P73452"
FT BINDING 148
FT /ligand="nitrate"
FT /ligand_id="ChEBI:CHEBI:17632"
FT /evidence="ECO:0000250|UniProtKB:P73452"
FT BINDING 193
FT /ligand="nitrate"
FT /ligand_id="ChEBI:CHEBI:17632"
FT /evidence="ECO:0000250|UniProtKB:P73452"
FT BINDING 237
FT /ligand="nitrate"
FT /ligand_id="ChEBI:CHEBI:17632"
FT /evidence="ECO:0000250|UniProtKB:P73452"
FT BINDING 266
FT /ligand="nitrate"
FT /ligand_id="ChEBI:CHEBI:17632"
FT /evidence="ECO:0000250|UniProtKB:P73452"
FT LIPID 26
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P73452"
FT LIPID 26
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000250|UniProtKB:P73452"
FT CONFLICT 100
FT /note="W -> C (in Ref. 1; CAA68041)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 440 AA; 48476 MW; 29937A41FB45CE9C CRC64;
MTHVSRRKFL FTTGAAAAAS ILVHGCTSNG SQSATTGEQA PSAAPAANVS AANAPKVETT
KAKLGFIPLT DAAPLIIAKE KGFFAKYGMT DIEVIKQKSW PVTRDNLKIG SSGGGIDGAH
ILSPMPYLMT INDKVPMYIL ARLNTNGQAI SVAEKFKELN VNLESKSLKD AAIKAKADKK
ALKMGITFPG GTHDLWMRYW LAAGGINPDQ DVVLEAVPPP QMVANMKVNT VDGFCVGEPW
NAQLVNQKIG YSALVTGELW KDHPEKAFSM RQDWIEQNPN AAQAILMAIL EAQQWCDKAE
NKEEMCKICS DRKYFNVAAA DIIERAKGNI DYGDGRKEQN FAHRMKFWAD NASYPYKSHD
IWFLTEDIRW GYLPKDTKVQ DIVNQVNKED LWKKAAKAIG VADAEIPASS SRGVETFFDG
VKFDPEKPEE YLNSLKIKKV
