ID   NRTB_PHOLA              Reviewed;         279 AA.
AC   Q51881;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Nitrate import permease protein NrtB {ECO:0000305};
GN   Name=nrtB {ECO:0000303|PubMed:7647306};
OS   Phormidium laminosum.
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales;
OC   Oscillatoriaceae; Phormidium.
OX   NCBI_TaxID=32059;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=OH-1-P-CL1;
RX   PubMed=7647306; DOI=10.1007/bf00021199;
RA   Merchan F., Kindle K.L., Llama M.J., Serra J.L., Fernandez E.;
RT   "Cloning and sequencing of the nitrate transport system from the
RT   thermophilic, filamentous cyanobacterium Phormidium laminosum: comparative
RT   analysis with the homologous system from Synechococcus sp. PCC 7942.";
RL   Plant Mol. Biol. 28:759-766(1995).
CC   -!- FUNCTION: Part of the ABC transporter complex NrtABCD involved in
CC       nitrate uptake. The complex is probably also involved in nitrite
CC       transport. Probably responsible for the translocation of the substrate
CC       across the membrane. {ECO:0000250|UniProtKB:P38044}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NrtC and
CC       NrtD), two transmembrane proteins (NrtB) and a solute-binding protein
CC       (NrtA). {ECO:0000250|UniProtKB:P38044}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P38044}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
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DR   EMBL; Z19598; CAA79657.1; -; Genomic_DNA.
DR   PIR; S56642; S56642.
DR   AlphaFoldDB; Q51881; -.
DR   SMR; Q51881; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR005889; NtrB.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR01183; ntrB; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Nitrate assimilation; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..279
FT                   /note="Nitrate import permease protein NrtB"
FT                   /id="PRO_0000060125"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          86..270
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   279 AA;  30205 MW;  5BE86C4B02EFC5F6 CRC64;
     MTADLSSPRR ARRSPSSYLG ALVGKTIRRS IPTAIAFFVL LGIWQLLCSG ENAACRPIQV
     VQESWDLIID PFYRGSGTDQ GLFWQIAASL QRVAVGYLMA AIAGIALGIL IGVSVLMFQA
     LDPIFQVLRT VPPLAWLPIS LAAFRDSQPA AIFVIFITAI WPIIINTAVG VQNIPQDYNN
     VARVLKLSRL DYFLKVLLPA TVPYIFTGLK IAIGLSWLAI VAAEMLTGGV GIGFFIWDAY
     NSGSNSEVIL AIIYVGLVGL LLNALVGFIA SRVVPDEQK