ID   NRTB_SYNE7              Reviewed;         279 AA.
AC   P38044; Q31NV1;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Nitrate import permease protein NrtB {ECO:0000305};
GN   Name=nrtB {ECO:0000303|PubMed:8437564}; OrderedLocusNames=Synpcc7942_1238;
OS   Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS   R2).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX   NCBI_TaxID=1140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=8437564; DOI=10.1007/bf00277112;
RA   Omata T., Andriesse X., Hirano A.;
RT   "Identification and characterization of a gene cluster involved in nitrate
RT   transport in the cyanobacterium Synechococcus sp. PCC7942.";
RL   Mol. Gen. Genet. 236:193-202(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7942 / FACHB-805;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=PCC 7942 / FACHB-805;
RX   PubMed=7767600; DOI=10.1093/oxfordjournals.pcp.a078751;
RA   Omata T.;
RT   "Structure, function and regulation of the nitrate transport system of the
RT   cyanobacterium Synechococcus sp. PCC7942.";
RL   Plant Cell Physiol. 36:207-213(1995).
CC   -!- FUNCTION: Part of the ABC transporter complex NrtABCD involved in
CC       nitrate uptake (PubMed:7767600, PubMed:8437564). The complex is
CC       probably also involved in nitrite transport (PubMed:7767600). Probably
CC       responsible for the translocation of the substrate across the membrane
CC       (PubMed:7767600). {ECO:0000269|PubMed:8437564,
CC       ECO:0000305|PubMed:7767600}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NrtC and
CC       NrtD), two transmembrane proteins (NrtB) and a solute-binding protein
CC       (NrtA). {ECO:0000305|PubMed:7767600}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:7767600};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Mutant is unable to grow on nitrate, but grows
CC       normally with nitrite or ammonium as the nitrogen source.
CC       {ECO:0000269|PubMed:8437564}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
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DR   EMBL; X61625; CAA43810.1; -; Genomic_DNA.
DR   EMBL; CP000100; ABB57268.1; -; Genomic_DNA.
DR   PIR; S30892; S30892.
DR   RefSeq; WP_011242626.1; NC_007604.1.
DR   AlphaFoldDB; P38044; -.
DR   SMR; P38044; -.
DR   STRING; 1140.Synpcc7942_1238; -.
DR   TCDB; 3.A.1.16.1; the atp-binding cassette (abc) superfamily.
DR   PRIDE; P38044; -.
DR   EnsemblBacteria; ABB57268; ABB57268; Synpcc7942_1238.
DR   KEGG; syf:Synpcc7942_1238; -.
DR   eggNOG; COG0600; Bacteria.
DR   HOGENOM; CLU_046113_1_1_3; -.
DR   OMA; CSIWPMI; -.
DR   OrthoDB; 1531811at2; -.
DR   BioCyc; SYNEL:SYNPCC7942_1238-MON; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR005889; NtrB.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR01183; ntrB; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Nitrate assimilation; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..279
FT                   /note="Nitrate import permease protein NrtB"
FT                   /id="PRO_0000060126"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          84..267
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   279 AA;  30207 MW;  5565FE001113A7AE CRC64;
     MTVTLRPPSS VRRSAWVKNP KLKPFLPYVV CLPIFLAIWQ VISAILGQDR LPGPINVVAN
     TWMPYIVEPF FDNGGTSKGL GLQILISLQR VAIGYLLAAC TGILVGGVLG MSKFLGKGLD
     PVIQVLRTVP PLAWFPISLM VFQDANTSAI FVIFITAIWP IIINTAVGIN QIPDDYNNVA
     RVLKLSKKDY ILNILIPSTV PYVFAGLRIA VGLAWLAIVA AEMLKADGGI GYFIWDAYNA
     GGDGSSSQII LAIFYVGLVG LSLDRLVAWV GRLVSPVSR