ID   NRTB_SYNY3              Reviewed;         275 AA.
AC   P73451;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Nitrate import permease protein NrtB {ECO:0000305};
GN   Name=nrtB; OrderedLocusNames=sll1451;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: Part of the ABC transporter complex NrtABCD involved in
CC       nitrate uptake. The complex is probably also involved in nitrite
CC       transport. Probably responsible for the translocation of the substrate
CC       across the membrane. {ECO:0000250|UniProtKB:P38044}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NrtC and
CC       NrtD), two transmembrane proteins (NrtB) and a solute-binding protein
CC       (NrtA). {ECO:0000250|UniProtKB:P38044}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P38044}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
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DR   EMBL; BA000022; BAA17491.1; -; Genomic_DNA.
DR   PIR; S77388; S77388.
DR   AlphaFoldDB; P73451; -.
DR   SMR; P73451; -.
DR   STRING; 1148.1652570; -.
DR   PaxDb; P73451; -.
DR   EnsemblBacteria; BAA17491; BAA17491; BAA17491.
DR   KEGG; syn:sll1451; -.
DR   eggNOG; COG0600; Bacteria.
DR   InParanoid; P73451; -.
DR   OMA; CSIWPMI; -.
DR   PhylomeDB; P73451; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR005889; NtrB.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR01183; ntrB; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Nitrate assimilation; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..275
FT                   /note="Nitrate import permease protein NrtB"
FT                   /id="PRO_0000060127"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          82..262
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   275 AA;  29720 MW;  47CDB9366185F99E CRC64;
     MASSTAGLRP RRKKNPLSFI YSPKVIRPAV AIAVLLVVWQ ILCSGEGSNL PSPVQVLEQT
     YPLILNPFFD NGGTDKGLGI QIFASLTRVA VGFSAAAVVG IALGILIGSS KFMYDALDPI
     FQVLRTIPPL AWLPIALAAL QEAEPSAIFV IFITAIWPIV INTTVGAQQV PQDYRNVSRV
     LKLSKSQYFF NILFPAAVPY IFTGLRIGIG LSWLAIVAAE MLIGGVGIGF FIWDAYNSSL
     ISEIIIALIY VGIVGLLLDR FIAFLESLVV PAEQK