NRTC_SYNY3
ID NRTC_SYNY3 Reviewed; 670 AA.
AC P73450;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Nitrate import ATP-binding protein NrtC {ECO:0000305};
DE EC=7.3.2.4 {ECO:0000250|UniProtKB:P38045};
GN Name=nrtC; OrderedLocusNames=sll1452;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- FUNCTION: Part of the ABC transporter complex NrtABCD involved in
CC nitrate uptake. The complex is probably also involved in nitrite
CC transport. Probably responsible for energy coupling to the transport
CC system. {ECO:0000250|UniProtKB:P38045}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + nitrate(out) = ADP + H(+) + nitrate(in) +
CC phosphate; Xref=Rhea:RHEA:13181, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17632, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.4;
CC Evidence={ECO:0000250|UniProtKB:P38045};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NrtC and
CC NrtD), two transmembrane proteins (NrtB) and a solute-binding protein
CC (NrtA). {ECO:0000250|UniProtKB:P38045}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P38045}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P38045}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:P38045}.
CC -!- DOMAIN: Contains an ATP-binding N-terminal domain and a NrtA-like C-
CC terminal domain, which are connected by a segment containing an
CC abundance of glutamine, alanine and positively charged amino acids. The
CC N-terminal domain is required for activity and the C-terminal domain is
CC involved in regulation of the activity. {ECO:0000250|UniProtKB:P38045}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC Nitrate/nitrite/cyanate uptake transporter (NitT) (TC 3.A.1.16) family.
CC {ECO:0000305}.
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DR EMBL; BA000022; BAA17490.1; -; Genomic_DNA.
DR PIR; S77387; S77387.
DR AlphaFoldDB; P73450; -.
DR SMR; P73450; -.
DR IntAct; P73450; 3.
DR STRING; 1148.1652569; -.
DR PaxDb; P73450; -.
DR EnsemblBacteria; BAA17490; BAA17490; BAA17490.
DR KEGG; syn:sll1452; -.
DR eggNOG; COG0715; Bacteria.
DR eggNOG; COG1116; Bacteria.
DR InParanoid; P73450; -.
DR OMA; PEAHIGQ; -.
DR PhylomeDB; P73450; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015414; F:ABC-type nitrate transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd13553; PBP2_NrtA_CpmA_like; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR005890; NO3_transporter_ATP-bd.
DR InterPro; IPR044527; NrtA/CpmA_ABC-bd_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01184; ntrCD; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Nitrate assimilation; Nucleotide-binding; Reference proteome; Translocase;
KW Transport.
FT CHAIN 1..670
FT /note="Nitrate import ATP-binding protein NrtC"
FT /id="PRO_0000092647"
FT DOMAIN 5..239
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT REGION 255..278
FT /note="Linker"
FT /evidence="ECO:0000250|UniProtKB:P38045"
FT REGION 279..670
FT /note="NrtA-like"
FT /evidence="ECO:0000250|UniProtKB:P38045"
FT BINDING 42..49
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 670 AA; 75101 MW; 03B47E6C7918AD14 CRC64;
MMPFIEIDHV DRIFPLPDGG RYIALKNIEL KISQGEFISL IGHSGCGKST LLNMISGLDK
PTFGGVIMEG KEITEPGPER MVVFQNYSLL PWLTVRQNIA LAVNRVLRDL PKPEQEKIID
DNIALVGLQR AAHKRPGELS GGMKQRVAIA RALSTRPKVL LLDEPFGALD ALTRGNLQER
LMEIVQESGV TCIMVTHDVD EALLLSDRVV MLTTGPEAHI GQILEVPIPR PRHRLEVVNH
PSYYALRGEM VYFLNQQKRA KKVGAVSQFA EAMGGNGLEK INLDLGFIPL TDCAPLVVAK
EKGFFQKHGL EQVNLVKEPS WQAIADGIRE RRLDGAQMVA GMPLALTLGM GGKTPLPMVT
AMVMSRNGNA ITLSKKFAEA GVKTLEDLRL KLAETPDQVS TLGMVHPASM QNLLLRYWLA
SGSIDPDQDI NLMRLPPPQM VSNLEAGNID GFCVGEPWNS YAVKQNLGYV IATDLDIWNG
HPEKVLGMRE EWVNKYPATH LALVKALLEA CEYCDDRRHR QEILDYLALP QYVGTSTEYI
SPGFLTEYDQ GNDAEAEMLL DFNQFYVKQS NYPSRSEGLW ILTQLARWGY IDFPKNWVEI
IERVRRPDLF GEACRHLGWP DLEGDHHNVS LFDGMVFTPN DPLGYIKRFT IHRDIQVTEI
LIDQIDQVNQ
