ID   NRTP_NOSP7              Reviewed;         500 AA.
AC   B2IZT6;
DT   10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Nitrate/nitrite transporter NrtP {ECO:0000305};
GN   Name=nrtP {ECO:0000303|PubMed:17090931};
GN   OrderedLocusNames=Npun_R1527 {ECO:0000312|EMBL:ACC80216.1};
OS   Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=63737;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29133 / PCC 73102;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA   Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT   "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION AS A PERMEASE, AND INDUCTION.
RC   STRAIN=ATCC 29133 / PCC 73102;
RX   PubMed=17090931; DOI=10.1271/bbb.60286;
RA   Aichi M., Yoshihara S., Yamashita M., Maeda S., Nagai K., Omata T.;
RT   "Characterization of the nitrate-nitrite transporter of the major
RT   facilitator superfamily (the nrtP gene product) from the cyanobacterium
RT   Nostoc punctiforme strain ATCC 29133.";
RL   Biosci. Biotechnol. Biochem. 70:2682-2689(2006).
CC   -!- FUNCTION: Transport system for both nitrate and nitrite, with much
CC       higher affinity for nitrate than for nitrite.
CC       {ECO:0000269|PubMed:17090931}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is repressed by ammonium.
CC       {ECO:0000269|PubMed:17090931}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR   EMBL; CP001037; ACC80216.1; -; Genomic_DNA.
DR   RefSeq; WP_012408234.1; NC_010628.1.
DR   AlphaFoldDB; B2IZT6; -.
DR   STRING; 63737.Npun_R1527; -.
DR   EnsemblBacteria; ACC80216; ACC80216; Npun_R1527.
DR   KEGG; npu:Npun_R1527; -.
DR   eggNOG; COG2223; Bacteria.
DR   HOGENOM; CLU_024204_4_1_3; -.
DR   OMA; IPCFMFA; -.
DR   OrthoDB; 1217595at2; -.
DR   PhylomeDB; B2IZT6; -.
DR   Proteomes; UP000001191; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IDA:UniProtKB.
DR   GO; GO:0015113; F:nitrite transmembrane transporter activity; IDA:UniProtKB.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:1902025; P:nitrate import; IDA:UniProtKB.
DR   GO; GO:0015707; P:nitrite transport; IDA:UniProtKB.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR044772; NO3_transporter.
DR   InterPro; IPR004737; NO3_transporter_NarK/NarU-like.
DR   PANTHER; PTHR23515; PTHR23515; 1.
DR   Pfam; PF07690; MFS_1; 2.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00886; 2A0108; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Nitrate assimilation;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..500
FT                   /note="Nitrate/nitrite transporter NrtP"
FT                   /id="PRO_0000439861"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        220..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        247..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        364..384
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        389..409
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        425..445
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        451..471
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   500 AA;  55034 MW;  9E5B9B549C875F25 CRC64;
     MLKKLFSFSD RYRILHQTWF AFFLTFVCWF NFAPFATTIG KELHLAPEQI KTLGICNLAL
     TIPARLIIGM LLDRFGPRIT YSILLMFAVV PCLATALAQD FNQLVISRLL MGIVGSGFVV
     GIRMVAEWFQ PKEMGIAQGI YGGWGNFGAF GAEFALPILA ISTSFFSGGA SNWRLAIALV
     GIITAIYGVI YYNTVQDTPR GKVYKKPKKN GSLEVTSIKS FWAMMISNFG LIFALGLLAW
     RLEQKKIHFL TLSQMYLTWL VLAGLFAYQS YKAWQVNREL LTGKKTYPVS ERFQFGQVAL
     LEFTYITNFG SELAAVSMLP AFFEKTFGLE HVVAGMIAAT YPFLNLVSRP SGGLISDKFG
     SRKWTMTIIS VGIGVSYLMA HFINSNWPIP VAIAVTMFAA YFAQAGCGAT YSIVPMIKKE
     ATGQIAGNVG AYGNFGGVVY LTIFSLTDAP TLFSTMGIAA LICAFMCAFF LKEPKGSFAP
     AYEGEASETA TKSSVFLTEE