NRTP_SYNP2
ID NRTP_SYNP2 Reviewed; 534 AA.
AC B1XLL7; Q9R6U5;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 2.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Nitrate/nitrite transporter NrtP {ECO:0000305};
DE AltName: Full=Nitrate/nitrite permease {ECO:0000303|PubMed:10572142};
GN Name=nrtP {ECO:0000303|PubMed:10572142};
GN Synonyms=narK {ECO:0000312|EMBL:ACA99310.1};
GN OrderedLocusNames=SYNPCC7002_A1313 {ECO:0000312|EMBL:ACA99310.1};
OS Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum
OS quadruplicatum).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC unclassified Synechococcus.
OX NCBI_TaxID=32049;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A PERMEASE, SUBCELLULAR
RP LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=10572142; DOI=10.1128/jb.181.23.7363-7372.1999;
RA Sakamoto T., Inoue-Sakamoto K., Bryant D.A.;
RT "A novel nitrate/nitrite permease in the marine Cyanobacterium
RT synechococcus sp. strain PCC 7002.";
RL J. Bacteriol. 181:7363-7372(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RA Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T.,
RA Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J.,
RA Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.;
RT "Complete sequence of Synechococcus sp. PCC 7002.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: High-efficiency transport system for both nitrate and
CC nitrite. {ECO:0000269|PubMed:10572142}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:10572142}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Transcribed in nitrate-grown cells, but not in cells grown
CC on urea or ammonia. Transcription is probably controlled by the global
CC nitrogen regulator NtcA. {ECO:0000269|PubMed:10572142}.
CC -!- DISRUPTION PHENOTYPE: Mutant can still grow on nitrate, but it has a
CC lower capacity for nitrate utilization, which limits the growth of the
CC cells. Mutant cannot consume nitrite as rapidly as the wild type cells
CC at pH 10. {ECO:0000269|PubMed:10572142}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACA99310.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF089813; AAD45941.1; -; Genomic_DNA.
DR EMBL; CP000951; ACA99310.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_041443465.1; NC_010475.1.
DR AlphaFoldDB; B1XLL7; -.
DR STRING; 32049.SYNPCC7002_A1313; -.
DR TCDB; 2.A.1.8.3; the major facilitator superfamily (mfs).
DR EnsemblBacteria; ACA99310; ACA99310; SYNPCC7002_A1313.
DR KEGG; syp:SYNPCC7002_A1313; -.
DR eggNOG; COG2223; Bacteria.
DR HOGENOM; CLU_024204_4_1_3; -.
DR Proteomes; UP000001688; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0015113; F:nitrite transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:1902025; P:nitrate import; IMP:UniProtKB.
DR GO; GO:0015707; P:nitrite transport; IMP:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR InterPro; IPR004737; NO3_transporter_NarK/NarU-like.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 2.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00886; 2A0108; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Nitrate assimilation;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..534
FT /note="Nitrate/nitrite transporter NrtP"
FT /id="PRO_0000439862"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 445..465
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 534 AA; 58153 MW; 6EA91F3ED78BDDEC CRC64;
MLGEMWSFNG RYKILHMTWF AFFLSFVVWF NFPPFATTIA QDFGLDKAQL GTIGLCNVAL
TVPARIIIGM LLDKYGPRLT YSLLLIYAAV PCLIFATAQS FNQLVLGRLL MGIVGAGFVI
GIRMVAEWFP PKDVGTAEGI YGGWGNFGSA FSAFTMVIFG IILAFLPGAF NFGQPESFKI
LFFPEFNTAI LNWRAAIAGT GIIAALYGML YYFSVSDTPP GKTYHRPKSA RGMEVTTKKD
FWFLLAMNLP LTLILMVLAW RLQKVNFLNG TGFAIAILAL VGLYLFQTYN CWTVNKDLMT
GKKRYAPEDR YEFSQVAILE LTYIVNFGSE LAVVTMLPAF FEGTFSLDKA TAGIIASSYA
FMNLMSRPGG GLISDKMGSR KWTMVVLTVG MGVGYLLMSS VAGTWPLAIA VLLTMACSFF
VQAAEGSTFA IVPLVKRRIT GQIAGNVGAY GNVGAVAYLT VLLLLTEASA GANGGEPVMA
TVNAGFFQVL GITGLIVAFL CAFFLKEPKG SFAEFHEGET EMTATPPIEE EATY
