NS1A_HASV8
ID NS1A_HASV8 Reviewed; 921 AA.
AC Q9IFX3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Non-structural polyprotein 1A;
DE Contains:
DE RecName: Full=VPg;
DE Contains:
DE RecName: Full=Protein p19;
DE Contains:
DE RecName: Full=Transmembrane protein 1A;
DE Contains:
DE RecName: Full=Serine protease p27;
DE Short=p27;
DE EC=3.4.21.-;
DE Contains:
DE RecName: Full=Protein p20';
GN Name=ORF1;
OS Human astrovirus-8 (HAstV-8).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC Stellavirales; Astroviridae; Mamastrovirus.
OX NCBI_TaxID=43358;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Yuc-8;
RX PubMed=11086120; DOI=10.1099/0022-1317-81-12-2891;
RA Mendez-Toss M., Romero-Guido P., Munguia M.E., Mendez E., Arias C.F.;
RT "Molecular analysis of a serotype 8 human astrovirus genome.";
RL J. Gen. Virol. 81:2891-2897(2000).
RN [2]
RP PROTEOLYTIC PROCESSING OF POLYPROTEIN.
RX PubMed=14557623; DOI=10.1128/jvi.77.21.11378-11384.2003;
RA Mendez E., Salas-Ocampo M.P., Munguia M.E., Arias C.F.;
RT "Protein products of the open reading frames encoding nonstructural
RT proteins of human astrovirus serotype 8.";
RL J. Virol. 77:11378-11384(2003).
CC -!- FUNCTION: [Non-structural polyprotein 1A]: Contains the viral protease
CC participating in the cleavage of the polyprotein into functional
CC products.
CC -!- FUNCTION: [VPg]: Protein covalently attached to the 5' extremity of the
CC genomic and subgenomic RNAs. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Ribosomal frameshifting; Named isoforms=2;
CC Name=nsp1a;
CC IsoId=Q9IFX3-1; Sequence=Displayed;
CC Name=nsp1ab;
CC IsoId=Q9IFX2-1; Sequence=External;
CC -!- PTM: Cleaved presumably by viral and host proteases.
CC -!- SIMILARITY: Belongs to the astroviridae polyprotein 1A family.
CC {ECO:0000305}.
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DR EMBL; AF260508; AAF85962.1; -; Genomic_RNA.
DR SMR; Q9IFX3; -.
DR MEROPS; S01.109; -.
DR Proteomes; UP000008629; Genome.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0070008; F:serine-type exopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR045835; Astro_1A.
DR InterPro; IPR045833; Astro_p19.
DR InterPro; IPR045836; Astro_VPg.
DR InterPro; IPR022068; Mamastrovirus_p20.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR Pfam; PF19415; Astro_1A; 1.
DR Pfam; PF19414; Astro_p19; 1.
DR Pfam; PF19416; Astro_VPg; 1.
DR Pfam; PF12285; DUF3621; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Host membrane; Hydrolase; Membrane; Protease;
KW Ribosomal frameshifting; Thiol protease; Transmembrane;
KW Transmembrane helix; Viral RNA replication.
FT CHAIN 1..921
FT /note="Non-structural polyprotein 1A"
FT /id="PRO_0000327340"
FT CHAIN 1..176
FT /note="Protein p19"
FT /evidence="ECO:0000255"
FT /id="PRO_0000327341"
FT CHAIN 177..420
FT /note="Transmembrane protein 1A"
FT /evidence="ECO:0000255"
FT /id="PRO_0000327342"
FT CHAIN 421..665
FT /note="Serine protease p27"
FT /evidence="ECO:0000255"
FT /id="PRO_0000327343"
FT CHAIN 666..756
FT /note="VPg"
FT /evidence="ECO:0000255"
FT /id="PRO_0000419598"
FT CHAIN 757..921
FT /note="Protein p20'"
FT /evidence="ECO:0000255"
FT /id="PRO_0000327344"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 753..813
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 900..921
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 780..800
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 462
FT /note="Charge relay system; for serine protease activity"
FT /evidence="ECO:0000250"
FT ACT_SITE 490
FT /note="Charge relay system; for serine protease activity"
FT /evidence="ECO:0000250"
FT ACT_SITE 552
FT /note="Charge relay system; for serine protease activity"
FT /evidence="ECO:0000250"
FT SITE 176..177
FT /note="Cleavage"
FT /evidence="ECO:0000255"
FT SITE 420..421
FT /note="Cleavage"
FT /evidence="ECO:0000255"
FT SITE 665..666
FT /note="Cleavage"
FT /evidence="ECO:0000255"
FT SITE 756..757
FT /note="Cleavage"
FT /evidence="ECO:0000255"
SQ SEQUENCE 921 AA; 103493 MW; 445175FC4EEE8571 CRC64;
MMALGEPYYS SKPDKDFNFG STMARRQMTP TMVTKLPKFV RNSPQAYDWI VRGLIFPTTG
KTYFQRVVVI TGGLEDGTYG SYAFNGSEWV EIYPIEHLNL MSSLKLIHKA NALQERLRLS
QEEKATLALD VQFLQHENVR LKELIPKPEP RKIQMKWIIV GAVLTFLSLI PGGYAQSQTN
NTIFTDMIAA CKYSTETLTE NLDLRIKLAL ANITINDKLD AVRQILNFAF VPRAHWLRTV
FYYIHYYEMW NIFMFVLAIG TVMRSARPGT DLITLATSHL SGFRMAVLPT IPFHTTMTLW
VMNTLMVCYY FDNLLAITMA ILAPILGIIF LCFMEDSNYV SQIRGLIATA VLIAGGHACL
TLTGTTTSLF VVILTCRFIR MATVFIGTRF EIRDANGKVV ATVPTRIKNV AFDFFQKLKQ
SGVRVGVNDF VVIKPGALCI IDTPEGKGTG FFSGNDIVTA AHVVGNNTFV SVCYEGLVYE
AKVRYMPEKD IAFITCPGDL HPTARLKLSK NPDYSCVTVM AYVNEDLVVS TATAMVHGNT
LSYAVRTQDG MSGAPVCDKY GRVLAVHQTN TGYTGGAVII DPADFHPVKA PSQVELLKEE
IERLKAQLNS AAENPVTVVT QQPIVTLEQK SVSDSDVVDL VRTAMEREMK VLRDEINGIL
APFLQKKKGK TKHGRGRVRR NLRKGVKLLT EEEYRELLEK GLDRETFLDL IDRIIGERSG
YPDYDDEDYY DEDDDGWGMV GDDVEFDYTE VINFDQAKPT PAPRTTKPKP CPEPKIEAQP
LDLSQKKEKQ PEHEQQVAKP TKPQKIEPQP YSQTYGKAPI WESYDFDWDE DDAKFILPAP
HRLTKADEIV LGSKIVKLRT IIETAIKTQN YSALPEAVFE LDKAAYEAGL EGFLQRVKSK
NKAPKNYKGP QKTKGPKTTT H
