NS1_BRSVA
ID NS1_BRSVA Reviewed; 136 AA.
AC Q65694; Q77L04;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 23-FEB-2022, entry version 72.
DE RecName: Full=Non-structural protein 1;
DE AltName: Full=Non-structural protein 1C;
GN Name=1C; Synonyms=NS1;
OS Bovine respiratory syncytial virus (strain A51908) (BRS).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus.
OX NCBI_TaxID=11247;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7844532; DOI=10.1099/0022-1317-76-1-193;
RA Pastey M.K., Samal S.K.;
RT "Nucleotide sequence analysis of the non-structural NS1 (1C) and NS2 (1B)
RT protein genes of bovine respiratory syncytial virus.";
RL J. Gen. Virol. 76:193-197(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=A51908, and ATCC 51908;
RX PubMed=11724268; DOI=10.1023/a:1011888019966;
RA Yunus A.S., Khattar S.K., Collins P.L., Samal S.K.;
RT "Rescue of bovine respiratory syncytial virus from cloned cDNA: entire
RT genome sequence of BRSV strain A51908.";
RL Virus Genes 23:157-164(2001).
RN [3]
RP FUNCTION.
RX PubMed=10954520; DOI=10.1128/jvi.74.18.8234-8242.2000;
RA Schlender J., Bossert B., Buchholz U., Conzelmann K.K.;
RT "Bovine respiratory syncytial virus nonstructural proteins NS1 and NS2
RT cooperatively antagonize alpha/beta interferon-induced antiviral
RT response.";
RL J. Virol. 74:8234-8242(2000).
RN [4]
RP FUNCTION.
RX PubMed=12885884; DOI=10.1128/jvi.77.16.8661-8668.2003;
RA Bossert B., Marozin S., Conzelmann K.K.;
RT "Nonstructural proteins NS1 and NS2 of bovine respiratory syncytial virus
RT block activation of interferon regulatory factor 3.";
RL J. Virol. 77:8661-8668(2003).
CC -!- FUNCTION: Plays a major role in antagonizing the type I IFN-mediated
CC antiviral response by degrading or inhibiting multiple cellular factors
CC required for either IFN induction or response pathways. Acts
CC cooperatively with NS2 to repress activation and nuclear translocation
CC of host IFN-regulatory factor IRF3. Also disrupts the association of
CC IRF3 with CREBBP. Interacts with host mitochondrial-associated membrane
CC (MAM) MAVS and prevents the interaction with DDX58/RIG-I. Interacts
CC with TRIM25 to suppress TRIM25-mediated DDX58 ubiquitination and
CC thereby DDX58-MAVS interaction. Together with NS2, participates in the
CC proteasomal degradation of host STAT2, IRF3, IRF7, TBK1 and DDX58/RIG-I
CC through a NS-degradasome involving CUL2 and Elongin-C. The degradasome
CC requires an intact mitochondrial MAVS. Decreases the levels of host
CC TRAF3 and IKBKE/IKK-epsilon. As functions other than disruptions of the
CC type I IFN-mediated antiviral signaling pathways, induces host SOCS1
CC and SOCS3 expression. Suppresses premature apoptosis by an NF-kappa-B-
CC dependent, interferon-independent mechanism and thus facilitates virus
CC growth. Additionally, NS1 may serve some inhibitory role in viral
CC transcription and RNA replication. Suppresses proliferation and
CC activation of host CD103+ CD8+ cytotoxic T-lymphocytes and Th17 helper
CC T-lymphocytes. {ECO:0000250|UniProtKB:P0DOE9}.
CC -!- SUBUNIT: Monomer. Homomultimer. Heteromultimer with NS2. Interacts with
CC the matrix protein M. Interacts with host ELOC and CUL2; this
CC interaction allows NS1 to form an active E3 ligase with ELOC and CUL2.
CC Interacts with host IRF3; this interaction leads to the disrupted
CC association of IRF3 with CREBBP and thus reduced binding of IRF3 to the
CC IFN-beta promoter. Interacts with host MAVS; this interaction prevents
CC MAVS binding to DDX58 and inhibits signaling pathway leading to
CC interferon production. Interacts with host TRIM25 (via SPRY domain);
CC this interaction suppresses DDX58 ubiquitination and results in
CC decreased interaction between DDX58 and MAVS.
CC {ECO:0000250|UniProtKB:P0DOE9}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250|UniProtKB:P0DOE9}.
CC Host mitochondrion {ECO:0000250|UniProtKB:P0DOE9}. Host nucleus
CC {ECO:0000250|UniProtKB:P0DOE9}. Note=Most NS1 resides in the
CC mitochondria as a heteromer with NS2. {ECO:0000250|UniProtKB:P0DOE9}.
CC -!- SIMILARITY: Belongs to the pneumovirus non-structural protein 1 family.
CC {ECO:0000305}.
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DR EMBL; U15937; AAA85671.1; -; mRNA.
DR EMBL; AF295543; AAL49392.1; -; Genomic_RNA.
DR EMBL; AF295544; AAL49403.1; -; Genomic_RNA.
DR RefSeq; NP_048048.1; NC_001989.1.
DR SMR; Q65694; -.
DR GeneID; 1489807; -.
DR KEGG; vg:1489807; -.
DR Proteomes; UP000007616; Genome.
DR GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0039504; P:suppression by virus of host adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0039564; P:suppression by virus of host JAK-STAT cascade via inhibition of STAT2 activity; IEA:UniProtKB-KW.
DR GO; GO:0039722; P:suppression by virus of host toll-like receptor signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0039540; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of host RIG-I activity; IEA:UniProtKB-KW.
DR GO; GO:0039548; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity; IEA:UniProtKB-KW.
DR GO; GO:0039557; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity; IEA:UniProtKB-KW.
DR GO; GO:0039545; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity; IEA:UniProtKB-KW.
DR GO; GO:0039723; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity; IEA:UniProtKB-KW.
DR InterPro; IPR005099; Pneumo_NS1.
DR Pfam; PF03438; Pneumo_NS1; 1.
PE 2: Evidence at transcript level;
KW Host cytoplasm; Host mitochondrion; Host nucleus; Host-virus interaction;
KW Inhibition of host adaptive immune response by virus;
KW Inhibition of host innate immune response by virus;
KW Inhibition of host interferon signaling pathway by virus;
KW Inhibition of host IRF3 by virus; Inhibition of host IRF7 by virus;
KW Inhibition of host MAVS by virus; Inhibition of host RIG-I by virus;
KW Inhibition of host RLR pathway by virus; Inhibition of host STAT2 by virus;
KW Inhibition of host TBK1 by virus; Inhibition of host TLR pathway by virus;
KW Interferon antiviral system evasion;
KW Modulation of host cell apoptosis by virus; Reference proteome;
KW Viral immunoevasion.
FT CHAIN 1..136
FT /note="Non-structural protein 1"
FT /id="PRO_0000142780"
SQ SEQUENCE 136 AA; 15247 MW; AEB6A89D7BED2D24 CRC64;
MGSETLSVIQ VRLRNIYDND KVALLKITCH TNRLILLTHT LAKSVIHTIK LSGIVFIHII
TSSDYCPTSD IINSANFTSM PILQNGGYIW ELMELTHCFQ TNGLIDDNCE ITFSKRLSDS
ELAKYSNQLS TLLGLN
