ID   NS1_JDNVP               Reviewed;         545 AA.
AC   Q90054;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 61.
DE   RecName: Full=Initiator protein NS1 {ECO:0000250|UniProtKB:P03134};
DE            Short=NS1;
DE            EC=3.1.21.- {ECO:0000250|UniProtKB:Q9PZT1};
DE            EC=3.6.4.12 {ECO:0000250|UniProtKB:Q9PZT1};
DE   AltName: Full=Non-structural protein 1;
DE   AltName: Full=Non-structural protein NS1;
GN   Name=NS1;
OS   Junonia coenia densovirus (isolate pBRJ/1990) (JcDNV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Quintoviricetes;
OC   Piccovirales; Parvoviridae; Densovirinae; Ambidensovirus.
OX   NCBI_TaxID=648250;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1413502; DOI=10.1016/0042-6822(92)90182-o;
RA   Dumas B., Jourdan M., Pascaud A.M., Bergoin M.;
RT   "Complete nucleotide sequence of the cloned infectious genome of Junonia
RT   coenia densovirus reveals an organization unique among parvoviruses.";
RL   Virology 191:202-222(1992).
CC   -!- FUNCTION: Multifunctional protein which displays endonuclease and
CC       helicase activities required for initiating and directing viral DNA
CC       replication. Also plays a role in viral packaging and transactivation
CC       of several promoters. Binds site-specifically to 2-3 approximate tandem
CC       copies within the origins of replication (Ori), unwinds this hairpin
CC       region and nicks one DNA strand thereby initiating the rolling circle
CC       replication (RCR). {ECO:0000250|UniProtKB:P03134}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000250|UniProtKB:P03134};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:P03134};
CC       Note=The endonuclease active site can probably bind other divalent
CC       cations. {ECO:0000250|UniProtKB:P03134};
CC   -!- SUBUNIT: Homooligomer. {ECO:0000250|UniProtKB:P03134}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250|UniProtKB:D0EZM8}.
CC   -!- DOMAIN: In the N-terminus, the endonuclease region is involved in
CC       binding to the origin of replication. In the middle, there are the
CC       ATPase and helicase activities (By similarity). The C-terminus probably
CC       contains a transactivation domain (By similarity).
CC       {ECO:0000250|UniProtKB:P03134, ECO:0000250|UniProtKB:Q9PZT1}.
CC   -!- SIMILARITY: Belongs to the parvoviruses initiator protein NS1 family.
CC       {ECO:0000305}.
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DR   EMBL; S47266; AAB23699.1; -; Genomic_DNA.
DR   PIR; B44054; B44054.
DR   RefSeq; NP_694824.1; NC_004284.1.
DR   GeneID; 955411; -.
DR   KEGG; vg:955411; -.
DR   Proteomes; UP000008294; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR014015; Helicase_SF3_DNA-vir.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001257; Parvovirus_NS1_helicase.
DR   Pfam; PF01057; Parvo_NS1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51206; SF3_HELICASE_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Covalent protein-DNA linkage; DNA replication; DNA-binding;
KW   Endonuclease; Helicase; Host nucleus; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Nucleotide-binding; Reference proteome; Transcription;
KW   Transcription regulation; Viral DNA replication; Viral genome packaging;
KW   Viral release from host cell.
FT   CHAIN           1..545
FT                   /note="Initiator protein NS1"
FT                   /id="PRO_0000222480"
FT   DOMAIN          346..545
FT                   /note="SF3 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT   REGION          1..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   545 AA;  63462 MW;  97CD825268ABC6AE CRC64;
     MNNGDTNRET DSTTRPNQDI IRESSGRTSP SEQCSMVANT SRKREWSHGG RGTMASIAKE
     SQENFQYMAE ELEKMGSEFF GYVTGQSIKP SSAYISDVII LRDIQLRDQC LDVLREYGRS
     RRNGLFGFSE EGDHIHVIHD CSYTNRSCRD IWISQVKPFG SVQKTGKPVK FIWEFKRTDW
     YDVFIYFFVR KRGERAIYVR GESGKIPSND ECVRWTREFK EREMVSSSDC TDYYECEQQE
     HKISRRSDAG SSNGRLYEKK AYSAGKFAYI RKKTKALLRK YYVSPVSAIC DVPEFRDDDL
     LCDPKNRDYI QAACDDFGKD LNAMSLREIY NLLTEDYNFT DEQELNPYAL FISSMKYDNL
     ENSLNIIIEL LKFQCNDDED LIVEFLTNLV NVLDRRIPKL NAFLIISPPS AGKNFFFDMI
     FGLLLSYGQL GQANRHNLFA FQEAPNKRVL LWNEPNYESS LTDTIKMMFG GDPYTVRVKN
     RMDAHVKRTP VIILTNNTVP FMYETAFSDR IIQYKWNAAP FLKDYELKPH PMTFFLLLSK
     YNITF