ID   NS2A_CVMA5              Reviewed;         261 AA.
AC   P19738;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   02-JUN-2021, entry version 82.
DE   RecName: Full=Non-structural protein 2a;
DE            Short=ns2a;
DE   AltName: Full=30 kDa accessory protein;
DE   AltName: Full=30 kDa non-structural protein;
DE   AltName: Full=ns2;
DE   AltName: Full=p30;
GN   ORFNames=2a;
OS   Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Embecovirus.
OX   NCBI_TaxID=11142;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2845655; DOI=10.1016/0042-6822(88)90512-0;
RA   Luytjes W., Bredenbeek P.J., Noten A.F.H., Horzinek M.C., Spaan W.J.M.;
RT   "Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA
RT   recombination between coronaviruses and influenza C virus.";
RL   Virology 166:415-422(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate C12 mutant;
RX   PubMed=9426441; DOI=10.1006/viro.1997.8877;
RA   Leparc-Goffart I., Hingley S.T., Chua M.M., Jiang X., Lavi E., Weiss S.R.;
RT   "Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is
RT   associated with a Q159L amino acid substitution in the spike protein.";
RL   Virology 239:1-10(1997).
CC   -!- FUNCTION: Not essential for virus replication in transformed murine
CC       cells.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the coronaviruses ns2a protein family.
CC       {ECO:0000305}.
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DR   EMBL; M23256; AAA46450.1; -; Genomic_RNA.
DR   EMBL; AF029248; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   PIR; A31165; MNIHMH.
DR   PDB; 4Z5V; X-ray; 3.05 A; A/B=2-199.
DR   PDBsum; 4Z5V; -.
DR   SMR; P19738; -.
DR   Proteomes; UP000007192; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007878; Coronavirus_NS2A.
DR   InterPro; IPR039573; NS2A-like.
DR   Pfam; PF05213; Corona_NS2A; 1.
DR   PIRSF; PIRSF003890; LigT_coronavirus; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Host cytoplasm; Reference proteome.
FT   CHAIN           1..261
FT                   /note="Non-structural protein 2a"
FT                   /id="PRO_0000106060"
FT   STRAND          9..14
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   HELIX           19..34
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   STRAND          38..41
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   STRAND          46..53
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   HELIX           59..70
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   HELIX           71..76
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   STRAND          78..88
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   STRAND          91..98
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   HELIX           100..112
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   STRAND          116..118
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   STRAND          126..135
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   STRAND          144..149
FT                   /evidence="ECO:0007829|PDB:4Z5V"
FT   STRAND          163..166
FT                   /evidence="ECO:0007829|PDB:4Z5V"
SQ   SEQUENCE   261 AA;  30393 MW;  7A08A913DABEA43B CRC64;
     MAFADKPNHF INFPLAQFSG FMGKYLKLQS QLVEMGLDCK LQKAPHVSIT LLDIKADQYK
     QVEFAIQEII DDLAAYEGDI VFDNPHMLGR CLVLDVRGFE ELHEDIVEIL RRRGCTADQS
     RHWIPHCTVA QFDEERETKG MQFYHKEPFY LKHNNLLTDA GLELVKIGSS KIDGFYCSEL
     SVWCGERLCY KPPTPKFSDI FGYCCIDKIR GDLEIGDLPQ DDEEAWAELS YHYQRNTYFF
     RHVHDNSIYF RTVCRMKGCM C