ID   NS2A_CVMJH              Reviewed;         265 AA.
AC   P26625;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Non-structural protein 2a;
DE            Short=ns2a;
DE   AltName: Full=30 kDa accessory protein;
DE   AltName: Full=30 kDa non-structural protein;
DE   AltName: Full=ns2;
DE   AltName: Full=p30;
GN   ORFNames=2a;
OS   Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Embecovirus.
OX   NCBI_TaxID=11144;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA / MRNA].
RX   PubMed=2168966; DOI=10.1128/jvi.64.10.4784-4791.1990;
RA   Schwarz B., Routledge E., Siddell S.G.;
RT   "Murine coronavirus nonstructural protein ns2 is not essential for virus
RT   replication in transformed cells.";
RL   J. Virol. 64:4784-4791(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2547994; DOI=10.1128/jvi.63.9.3729-3736.1989;
RA   Shieh C.-K., Lee H.-J., Yokomori K., la Monica N., Makino S., Lai M.M.C.;
RT   "Identification of a new transcriptional initiation site and the
RT   corresponding functional gene 2b in the murine coronavirus RNA genome.";
RL   J. Virol. 63:3729-3736(1989).
CC   -!- FUNCTION: Not essential for virus replication in transformed murine
CC       cells.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the coronaviruses ns2a protein family.
CC       {ECO:0000305}.
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DR   EMBL; M34035; AAA46464.1; -; Genomic_RNA.
DR   EMBL; M28348; AAA46441.1; -; Genomic_RNA.
DR   EMBL; M57954; AAA46463.1; -; mRNA.
DR   PIR; A36542; MNIHJH.
DR   RefSeq; YP_209231.1; AC_000192.1.
DR   SMR; P26625; -.
DR   KEGG; vg:3283264; -.
DR   Proteomes; UP000007193; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007878; Coronavirus_NS2A.
DR   InterPro; IPR009097; Cyclic_Pdiesterase.
DR   InterPro; IPR039573; NS2A-like.
DR   Pfam; PF05213; Corona_NS2A; 1.
DR   PIRSF; PIRSF003890; LigT_coronavirus; 1.
DR   SUPFAM; SSF55144; SSF55144; 1.
PE   2: Evidence at transcript level;
KW   Host cytoplasm.
FT   CHAIN           1..265
FT                   /note="Non-structural protein 2a"
FT                   /id="PRO_0000106061"
FT   CONFLICT        177
FT                   /note="G -> D (in Ref. 2; AAA46441)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   265 AA;  30874 MW;  8B1C66E28D9AE95B CRC64;
     MAARMAFADK PNHFINFPLA QFSGFMGKYL KLQSQLVEMG LDCKLQKVPH VSITLLDIKA
     DQYKQVEFAI QEIIDDLAAY EGDIVFDNPH MLGRCLVLDV KGFEELHEDI VEILRRRGCT
     ADQSRQWIPH CTVAQFDEEK EIKEMQFYFK LPFYLKHNNL LTDARLELVK IGSSKVGGFY
     CSELSIWCGE RLCYKPPTPK FSDIFGYCCI DKIRGDLEIG DLPPDDEEAW AELSYHYQRN
     TYFFRHVHDN SIYFRTVCRM KGCMC