NS2_BRSVA
ID NS2_BRSVA Reviewed; 124 AA.
AC Q65695; Q77KZ3; Q77L03;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 23-FEB-2022, entry version 67.
DE RecName: Full=Non-structural protein 2;
DE Short=NS2;
DE AltName: Full=Non-structural protein 1B;
GN Name=1B; Synonyms=NS2;
OS Bovine respiratory syncytial virus (strain A51908) (BRS).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus.
OX NCBI_TaxID=11247;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7844532; DOI=10.1099/0022-1317-76-1-193;
RA Pastey M.K., Samal S.K.;
RT "Nucleotide sequence analysis of the non-structural NS1 (1C) and NS2 (1B)
RT protein genes of bovine respiratory syncytial virus.";
RL J. Gen. Virol. 76:193-197(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A51908, and ATCC 51908;
RX PubMed=11724268; DOI=10.1023/a:1011888019966;
RA Yunus A.S., Khattar S.K., Collins P.L., Samal S.K.;
RT "Rescue of bovine respiratory syncytial virus from cloned cDNA: entire
RT genome sequence of BRSV strain A51908.";
RL Virus Genes 23:157-164(2001).
CC -!- FUNCTION: Plays a major role in antagonizing the type I IFN-mediated
CC antiviral response. Acts cooperatively with NS1 to repress activation
CC and nuclear translocation of host IFN-regulatory factor IRF3. Interacts
CC with the host cytoplasmic sensor of viral nucleic acids DDX58/RIG-I and
CC prevents the interaction with its downstream partner MAVS. Together
CC with NS2, participates in the proteasomal degradation of host STAT2,
CC IRF3, IRF7, TBK1 and DDX58 through a NS-degradasome involving CUL2 and
CC Elongin-C. The degradasome requires an intact mitochondrial MAVS.
CC Induces host SOCS1 expression. Induces activation of NF-kappa-B.
CC Suppresses premature apoptosis by an NF-kappa-B-dependent, interferon-
CC independent mechanism promoting continued viral replication.
CC {ECO:0000250|UniProtKB:P04543}.
CC -!- SUBUNIT: Monomer (instable). Homomultimer. Heteromultimer with NS1.
CC Interacts with host DDX58 (via N-terminus); this interaction prevents
CC host signaling pathway involved in interferon production. Interacts
CC with host MAP1B/microtubule-associated protein 1B.
CC {ECO:0000250|UniProtKB:P04543}.
CC -!- SUBCELLULAR LOCATION: Host mitochondrion
CC {ECO:0000250|UniProtKB:P04543}. Note=Most NS2 resides in the
CC mitochondria as a heteromer with NS1. {ECO:0000250|UniProtKB:P04543}.
CC -!- DOMAIN: The DNLP motif has IFN suppressive functions like binding to
CC host MAP1B. {ECO:0000250|UniProtKB:P04543}.
CC -!- SIMILARITY: Belongs to the pneumovirus non-structural protein 2 family.
CC {ECO:0000305}.
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DR EMBL; U15938; AAA85672.1; -; mRNA.
DR EMBL; AF295543; AAL49393.1; -; Genomic_RNA.
DR EMBL; AF295544; AAL49404.1; -; Genomic_RNA.
DR SMR; Q65695; -.
DR Proteomes; UP000007616; Genome.
DR GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0039564; P:suppression by virus of host JAK-STAT cascade via inhibition of STAT2 activity; IEA:UniProtKB-KW.
DR GO; GO:0039722; P:suppression by virus of host toll-like receptor signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0039540; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of host RIG-I activity; IEA:UniProtKB-KW.
DR GO; GO:0039548; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity; IEA:UniProtKB-KW.
DR GO; GO:0039557; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity; IEA:UniProtKB-KW.
DR GO; GO:0039723; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity; IEA:UniProtKB-KW.
DR InterPro; IPR004336; RSV_NS2.
DR Pfam; PF03113; RSV_NS2; 1.
PE 2: Evidence at transcript level;
KW Host mitochondrion; Host-virus interaction;
KW Inhibition of host innate immune response by virus;
KW Inhibition of host interferon signaling pathway by virus;
KW Inhibition of host IRF3 by virus; Inhibition of host IRF7 by virus;
KW Inhibition of host RIG-I by virus; Inhibition of host RLR pathway by virus;
KW Inhibition of host STAT2 by virus; Inhibition of host TBK1 by virus;
KW Inhibition of host TLR pathway by virus;
KW Interferon antiviral system evasion;
KW Modulation of host cell apoptosis by virus; Reference proteome;
KW Viral immunoevasion.
FT CHAIN 1..124
FT /note="Non-structural protein 2"
FT /id="PRO_0000142786"
FT MOTIF 121..124
FT /note="DLNP; interaction with MAP1B"
FT /evidence="ECO:0000250|UniProtKB:P04543"
FT VARIANT 18
FT /note="I -> M (in strain: ATCC 51908)"
FT VARIANT 91
FT /note="L -> F (in strain: ATCC 51908)"
FT VARIANT 113
FT /note="H -> N (in strain: ATCC 51908)"
SQ SEQUENCE 124 AA; 14576 MW; B47548B0D5FB8FFD CRC64;
MSTPNPETTA QRLIVNDIRP LSIETEIISL TKDIITHTFI YLINHECIVR KLDERQATFT
FLVNYEMKLL HKVGSTKYNK YTEYNRKYGT LPMPIFINHD GFLECIGIKP TRHTPIIYKY
DLNP
