NS2_HRSVL
ID NS2_HRSVL Reviewed; 124 AA.
AC Q86305;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Non-structural protein 2;
DE Short=NS2;
DE AltName: Full=Non-structural protein 1B;
GN Name=1B; Synonyms=NS2;
OS Human respiratory syncytial virus A (strain Long).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus.
OX NCBI_TaxID=11260;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Mazumder B., Dupuy L.C., McLean T., Barik S.;
RL Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP REVIEW.
RX PubMed=31622448; DOI=10.1371/journal.ppat.1007984;
RA Sedeyn K., Schepens B., Saelens X.;
RT "Respiratory syncytial virus nonstructural proteins 1 and 2: Exceptional
RT disrupters of innate immune responses.";
RL PLoS Pathog. 15:e1007984-e1007984(2019).
RN [3]
RP REVIEW.
RX PubMed=32509597; DOI=10.3389/fcimb.2020.00225;
RA Thornhill E.M., Verhoeven D.;
RT "Respiratory Syncytial Virus's Non-structural Proteins: Masters of
RT Interference.";
RL Front. Cell. Infect. Microbiol. 10:225-225(2020).
CC -!- FUNCTION: Plays a major role in antagonizing the type I IFN-mediated
CC antiviral response. Acts cooperatively with NS1 to repress activation
CC and nuclear translocation of host IFN-regulatory factor IRF3. Interacts
CC with the host cytoplasmic sensor of viral nucleic acids DDX58/RIG-I and
CC prevents the interaction with its downstream partner MAVS. Together
CC with NS2, participates in the proteasomal degradation of host STAT2,
CC IRF3, IRF7, TBK1 and DDX58 through a NS-degradasome involving CUL2 and
CC Elongin-C. The degradasome requires an intact mitochondrial MAVS.
CC Induces host SOCS1 expression. Induces activation of NF-kappa-B.
CC Suppresses premature apoptosis by an NF-kappa-B-dependent, interferon-
CC independent mechanism promoting continued viral replication.
CC {ECO:0000250|UniProtKB:P04543}.
CC -!- SUBUNIT: Monomer (instable). Homomultimer. Heteromultimer with NS1.
CC Interacts with host DDX58 (via N-terminus); this interaction prevents
CC host signaling pathway involved in interferon production. Interacts
CC with host MAP1B/microtubule-associated protein 1B.
CC {ECO:0000250|UniProtKB:P04543}.
CC -!- SUBCELLULAR LOCATION: Host mitochondrion
CC {ECO:0000250|UniProtKB:P04543}. Note=Most NS2 resides in the
CC mitochondria as a heteromer with NS1. {ECO:0000250|UniProtKB:P04543}.
CC -!- DOMAIN: The DNLP motif has IFN suppressive functions like binding to
CC host MAP1B. {ECO:0000250|UniProtKB:P04543}.
CC -!- SIMILARITY: Belongs to the pneumovirus non-structural protein 2 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U35029; AAA79090.1; -; mRNA.
DR PDB; 7LDK; X-ray; 2.82 A; A/B/C=1-124.
DR PDBsum; 7LDK; -.
DR SMR; Q86305; -.
DR GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0039564; P:suppression by virus of host JAK-STAT cascade via inhibition of STAT2 activity; IEA:UniProtKB-KW.
DR GO; GO:0039722; P:suppression by virus of host toll-like receptor signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0039540; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of host RIG-I activity; IEA:UniProtKB-KW.
DR GO; GO:0039548; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity; IEA:UniProtKB-KW.
DR GO; GO:0039557; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity; IEA:UniProtKB-KW.
DR GO; GO:0039723; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity; IEA:UniProtKB-KW.
DR InterPro; IPR004336; RSV_NS2.
DR Pfam; PF03113; RSV_NS2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host mitochondrion; Host-virus interaction;
KW Inhibition of host innate immune response by virus;
KW Inhibition of host interferon signaling pathway by virus;
KW Inhibition of host IRF3 by virus; Inhibition of host IRF7 by virus;
KW Inhibition of host RIG-I by virus; Inhibition of host RLR pathway by virus;
KW Inhibition of host STAT2 by virus; Inhibition of host TBK1 by virus;
KW Inhibition of host TLR pathway by virus;
KW Interferon antiviral system evasion;
KW Modulation of host cell apoptosis by virus; Viral immunoevasion.
FT CHAIN 1..124
FT /note="Non-structural protein 2"
FT /id="PRO_0000142791"
FT MOTIF 121..124
FT /note="DLNP; interaction with MAP1B"
FT /evidence="ECO:0000250|UniProtKB:P04543"
FT HELIX 23..34
FT /evidence="ECO:0007829|PDB:7LDK"
FT HELIX 37..49
FT /evidence="ECO:0007829|PDB:7LDK"
FT HELIX 54..88
FT /evidence="ECO:0007829|PDB:7LDK"
FT STRAND 93..99
FT /evidence="ECO:0007829|PDB:7LDK"
FT STRAND 102..109
FT /evidence="ECO:0007829|PDB:7LDK"
FT STRAND 111..114
FT /evidence="ECO:0007829|PDB:7LDK"
FT HELIX 119..121
FT /evidence="ECO:0007829|PDB:7LDK"
SQ SEQUENCE 124 AA; 14705 MW; 98657318FEB744E1 CRC64;
MDTTHNDTTP QRLMITDMRP LSLETTITSL TRDIITHRFI YLINHECIVR KLDERQATFT
FLVNYEMKLL HKVGSTKYKK YTEYNTKYGT FPMPIFINHD GFLECIGIKP TKHTPIIYKY
DLNP
