NS2_MUMIP
ID NS2_MUMIP Reviewed; 188 AA.
AC P0DJZ2;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 14-MAY-2014, sequence version 1.
DT 02-JUN-2021, entry version 28.
DE RecName: Full=Non-structural protein NS2;
GN Name=NS2;
OS Murine minute virus (strain MVM prototype) (MVM) (Murine minute virus
OS (strain MVM(p))).
OC Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Quintoviricetes;
OC Piccovirales; Parvoviridae; Parvovirinae; Protoparvovirus.
OX NCBI_TaxID=648235;
OH NCBI_TaxID=10090; Mus musculus (Mouse).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6298737; DOI=10.1093/nar/11.4.999;
RA Astell C.R., Thomson M., Merchlinsky M., Ward D.C.;
RT "The complete DNA sequence of minute virus of mice, an autonomous
RT parvovirus.";
RL Nucleic Acids Res. 11:999-1018(1983).
RN [2]
RP FUNCTION.
RX PubMed=8419637; DOI=10.1128/jvi.67.2.1034-1043.1993;
RA Naeger L.K., Salome N., Pintel D.J.;
RT "NS2 is required for efficient translation of viral mRNA in minute virus of
RT mice-infected murine cells.";
RL J. Virol. 67:1034-1043(1993).
RN [3]
RP INTERACTION WITH HOST XPO1.
RX PubMed=10438867; DOI=10.1128/jvi.73.9.7769-7779.1999;
RA Bodendorf U., Cziepluch C., Jauniaux J.C., Rommelaere J., Salome N.;
RT "Nuclear export factor CRM1 interacts with nonstructural proteins NS2 from
RT parvovirus minute virus of mice.";
RL J. Virol. 73:7769-7779(1999).
RN [4]
RP FUNCTION.
RX PubMed=12239307; DOI=10.1128/jvi.76.20.10307-10319.2002;
RA Eichwald V., Daeffler L., Klein M., Rommelaere J., Salome N.;
RT "The NS2 proteins of parvovirus minute virus of mice are required for
RT efficient nuclear egress of progeny virions in mouse cells.";
RL J. Virol. 76:10307-10319(2002).
RN [5]
RP INTERACTION WITH HOST SNRPN, AND SUBCELLULAR LOCATION.
RX PubMed=12021369; DOI=10.1128/jvi.76.12.6364-6369.2002;
RA Young P.J., Jensen K.T., Burger L.R., Pintel D.J., Lorson C.L.;
RT "Minute virus of mice small nonstructural protein NS2 interacts and
RT colocalizes with the Smn protein.";
RL J. Virol. 76:6364-6369(2002).
RN [6]
RP FUNCTION.
RX PubMed=16160164; DOI=10.1128/jvi.79.19.12375-12381.2005;
RA Choi E.Y., Newman A.E., Burger L., Pintel D.;
RT "Replication of minute virus of mice DNA is critically dependent on
RT accumulated levels of NS2.";
RL J. Virol. 79:12375-12381(2005).
CC -!- FUNCTION: Plays an essential role in viral protein synthesis. Plays
CC also a role in active nuclear export of mature particles before host
CC cell lysis, by interacting with host XPO1.
CC {ECO:0000269|PubMed:12239307, ECO:0000269|PubMed:16160164,
CC ECO:0000269|PubMed:8419637}.
CC -!- SUBUNIT: Interacts with host SNRPN. Interacts with host XPO1.
CC {ECO:0000269|PubMed:10438867, ECO:0000269|PubMed:12021369}.
CC -!- INTERACTION:
CC P0DJZ2; Q6P5F9: Xpo1; Xeno; NbExp=2; IntAct=EBI-9673535, EBI-2550236;
CC P0DJZ2; Q80U96: Xpo1; Xeno; NbExp=2; IntAct=EBI-9673535, EBI-9517348;
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:12021369}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=NS2Y;
CC IsoId=P0DJZ2-1; Sequence=Displayed;
CC Name=NS2P;
CC IsoId=P0DJZ2-2; Sequence=VSP_054677;
CC Name=NS2L;
CC IsoId=P0DJZ2-3; Sequence=VSP_054678;
CC -!- MISCELLANEOUS: [Isoform NS2Y]: Splicing isoform (approximately 15% of
CC the molecules).
CC -!- MISCELLANEOUS: [Isoform NS2P]: Major splicing isoform (approximately
CC 85% of the molecules). {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform NS2L]: Minor splicing isoform. {ECO:0000305}.
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DR EMBL; J02275; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PDB; 6A38; X-ray; 2.69 A; D=77-94.
DR PDB; 6A3A; X-ray; 2.30 A; D=77-94.
DR PDB; 6A3B; X-ray; 2.51 A; D=76-93.
DR PDB; 6A3C; X-ray; 2.35 A; D=77-95.
DR PDB; 6A3E; X-ray; 2.70 A; D=76-95.
DR PDB; 6CIT; X-ray; 2.03 A; D=77-93.
DR PDB; 6KFT; X-ray; 2.51 A; D=76-93.
DR PDBsum; 6A38; -.
DR PDBsum; 6A3A; -.
DR PDBsum; 6A3B; -.
DR PDBsum; 6A3C; -.
DR PDBsum; 6A3E; -.
DR PDBsum; 6CIT; -.
DR PDBsum; 6KFT; -.
DR SMR; P0DJZ2; -.
DR IntAct; P0DJZ2; 2.
DR Proteomes; UP000007019; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Host nucleus; Reference proteome.
FT CHAIN 1..188
FT /note="Non-structural protein NS2"
FT /id="PRO_0000429047"
FT VAR_SEQ 183..188
FT /note="YDGASS -> LGASWLQVPGTREQP (in isoform NS2L)"
FT /evidence="ECO:0000305"
FT /id="VSP_054678"
FT VAR_SEQ 183..188
FT /note="YDGASS -> PEITWF (in isoform NS2P)"
FT /evidence="ECO:0000305"
FT /id="VSP_054677"
FT HELIX 78..87
FT /evidence="ECO:0007829|PDB:6CIT"
SQ SEQUENCE 188 AA; 21447 MW; 563514AE8357D2AA CRC64;
MAGNAYSDEV LGATNWLKEK SNQEVFSFVF KNENVQLNGK DIGWNSYKKE LQEDELKSLQ
RGAETTWDQS EDMEWETTVD EMTKKFGTLT IHDTEKYASQ PELCTNSTCI GSRGPGFRAL
EHTKYSCCGH CRNPEHWGSW FQSLPRWSTE PNLVRDRGGF ESVLRCGTVE ERLQRAAELG
LRYDGASS
