ID   NS3B_CVCBG              Reviewed;         250 AA.
AC   Q7T6T1;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   23-FEB-2022, entry version 39.
DE   RecName: Full=Non-structural protein 3b;
DE            Short=ns3b;
DE   AltName: Full=Accessory protein 3b;
DE   AltName: Full=Protein X2;
GN   ORFNames=3b;
OS   Canine coronavirus (strain BGF10) (CCoV) (Canine enteric coronavirus).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=441619;
OH   NCBI_TaxID=9615; Canis lupus familiaris (Dog) (Canis familiaris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15177889; DOI=10.1016/j.virusres.2004.02.038;
RA   Sanchez-Morgado J.M., Poynter S., Morris T.H.;
RT   "Molecular characterization of a virulent canine coronavirus BGF strain.";
RL   Virus Res. 104:27-31(2004).
CC   -!- FUNCTION: May be a virulence factor.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the coronaviruses NS3b protein family.
CC       {ECO:0000305}.
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DR   EMBL; AY342160; AAQ17222.1; -; Genomic_RNA.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR004293; Coronavirus_Orf3b.
DR   Pfam; PF03053; Corona_NS3b; 1.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..250
FT                   /note="Non-structural protein 3b"
FT                   /id="PRO_0000289888"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          40..130
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01311"
FT   DOMAIN          134..213
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01312"
SQ   SEQUENCE   250 AA;  28356 MW;  119206194F1AA69B CRC64;
     MIGGLFLNTL SFVIVSNHVV NNTANVHHIQ QEHVIVQQTQ IVSARTQNYY PEFSIAVLFV
     SFLALYRSTN FKTCVGILMF KIVSMTLIGP MLTAYGYYID GIVTTTVLAL RFIYLSYFWY
     VNNRFEFVLY NTTTLMFVHG RAAPFMRSSH SSIYVTLYGG INYMFVNDLT LHFVDPMLVS
     IAIRGLAHAD LTVVRAVELL NGDFIYVFSQ EPVVGVYNAA FSQAVLNEID LKEEVEDHVY
     DVPSGINCHR