ID   NS3B_CVPFS              Reviewed;         244 AA.
AC   P22656;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   23-FEB-2022, entry version 57.
DE   RecName: Full=Non-structural protein 3b;
DE            Short=ns3b;
DE   AltName: Full=Accessory protein 3b;
DE   AltName: Full=Non-structural protein 3-1;
DE   AltName: Full=X2b protein;
GN   ORFNames=3b;
OS   Porcine transmissible gastroenteritis coronavirus (strain FS772/70) (TGEV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=11150;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2546515; DOI=10.1007/bf01311354;
RA   Britton P., Lopez-Otin C., Martin-Alonso J.M., Parra F.;
RT   "Sequence of the coding regions from the 3.0 kb and 3.9 kb mRNA. Subgenomic
RT   species from a virulent isolate of transmissible gastroenteritis virus.";
RL   Arch. Virol. 105:165-178(1989).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the coronaviruses NS3b protein family.
CC       {ECO:0000305}.
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DR   EMBL; X14551; CAA32687.1; -; Genomic_RNA.
DR   PIR; B60076; B60076.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR004293; Coronavirus_Orf3b.
DR   Pfam; PF03053; Corona_NS3b; 1.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..244
FT                   /note="Non-structural protein 3b"
FT                   /id="PRO_0000106076"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          41..131
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01311"
FT   DOMAIN          135..214
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01312"
SQ   SEQUENCE   244 AA;  27624 MW;  2717B86EA0300A17 CRC64;
     MIGGLFLNTL SFVIVSNHSI VNNTANVHHI QQERVIVQQH QVVSAITQNY YPEFSIAVLF
     VSFLALYRST NFKTCVGILM FKILSMTLLG PMLIAYGYYI DGIVTTTVLS LRFAYLAYFW
     YVNSRFEFIL YNTTTLMFVH GRAAPFKRSS HSSIYVTLYG GINYMFVNDL TLHFVDPMLV
     SIAIRGLAHA DLTVVRAVEL LNGDFIYVFS QEPVVGVYNA AFSQAVLNEI DLKEEEGDRT
     YDVS