ID   NS3B_CVPPU              Reviewed;         244 AA.
AC   P09047; Q85086;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 2.
DT   02-JUN-2021, entry version 65.
DE   RecName: Full=Putative non-structural protein 3b;
DE            Short=ns3b;
DE   AltName: Full=Accessory protein 3b;
DE   AltName: Full=Non-structural protein 3-1;
DE   AltName: Full=X2b protein;
GN   ORFNames=3b;
OS   Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=11151;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2825819; DOI=10.1016/0300-9084(87)90178-7;
RA   Rasschaert D., Gelfi J., Laude H.;
RT   "Enteric coronavirus TGEV: partial sequence of the genomic RNA, its
RT   organization and expression.";
RL   Biochimie 69:591-600(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2548340; DOI=10.1007/bf00125345;
RA   Kapke P.A., Tung F.Y., Brian D.A.;
RT   "Nucleotide sequence between the peplomer and matrix protein genes of the
RT   porcine transmissible gastroenteritis coronavirus identifies three large
RT   open reading frames.";
RL   Virus Genes 2:293-294(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2549745; DOI=10.1016/0168-1702(89)90008-7;
RA   Wesley R.D., Cheung A.K., Michael D.D., Woods R.D.;
RT   "Nucleotide sequence of coronavirus TGEV genomic RNA: evidence for 3 mRNA
RT   species between the peplomer and matrix protein genes.";
RL   Virus Res. 13:87-100(1989).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate PUR46-MAD;
RX   PubMed=10805807; DOI=10.1073/pnas.97.10.5516;
RA   Almazan F., Gonzalez J.M., Penzes Z., Izeta A., Calvo E., Plana-Duran J.,
RA   Enjuanes L.;
RT   "Engineering the largest RNA virus genome as an infectious bacterial
RT   artificial chromosome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:5516-5521(2000).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the coronaviruses NS3b protein family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA29671.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X06371; CAA29671.1; ALT_INIT; Genomic_RNA.
DR   EMBL; X12800; CAA31287.1; -; Genomic_DNA.
DR   EMBL; AJ271965; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   PIR; S01740; S01740.
DR   PIR; S04890; S04890.
DR   Proteomes; UP000001440; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR004293; Coronavirus_Orf3b.
DR   Pfam; PF03053; Corona_NS3b; 1.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   5: Uncertain;
KW   Host membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..244
FT                   /note="Putative non-structural protein 3b"
FT                   /id="PRO_0000106075"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          41..131
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01311"
FT   DOMAIN          135..214
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01312"
FT   CONFLICT        8
FT                   /note="S -> N (in Ref. 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        31
FT                   /note="Q -> K (in Ref. 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        41
FT                   /note="Q -> H (in Ref. 4)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        59
FT                   /note="L -> P (in Ref. 2; CAA31287)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        114
FT                   /note="V -> A (in Ref. 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        147
FT                   /note="M -> K (in Ref. 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        239
FT                   /note="H -> R (in Ref. 3)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   244 AA;  27724 MW;  61E53A5D3F8BFED8 CRC64;
     MIGGLFLSTL SFVIVSNHSI VNNTANVHHI QQERVIVQQH QVVSARTQNY YPEFSIAVLF
     VSFLALYRST NFKTCVGILM FKILSMTLLG PMLIAYGYYI DGIVTTTVLS LRFVYLAYFW
     YVNSRFEFIL YNTTTLMFVH GRAAPFMRSS HSSIYVTLYG GINYMFVNDL TLHFVDPMLV
     SIAIRGLAHA DLTVVRAVEL LNGDFIYVFS QEPVVGVYNA AFSQAVLNEI DLKEEEEDHT
     YDVS