ID   NS3B_CVPRM              Reviewed;         244 AA.
AC   P24414;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   23-FEB-2022, entry version 56.
DE   RecName: Full=Non-structural protein 3b;
DE            Short=ns3b;
DE   AltName: Full=Accessory protein 3b;
DE   AltName: Full=Non-structural protein 3-1;
GN   ORFNames=3b;
OS   Porcine respiratory coronavirus (strain RM4) (PRCoV) (PRCV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=11148;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2174956; DOI=10.1099/0022-1317-71-11-2599;
RA   Rasschaert D., Duarte M., Laude H.;
RT   "Porcine respiratory coronavirus differs from transmissible gastroenteritis
RT   virus by a few genomic deletions.";
RL   J. Gen. Virol. 71:2599-2607(1990).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the coronaviruses NS3b protein family.
CC       {ECO:0000305}.
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DR   EMBL; Z24675; CAA80838.1; -; Genomic_RNA.
DR   PIR; B36607; B36607.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR004293; Coronavirus_Orf3b.
DR   Pfam; PF03053; Corona_NS3b; 1.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..244
FT                   /note="Non-structural protein 3b"
FT                   /id="PRO_0000106077"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          41..131
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01311"
FT   DOMAIN          135..214
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01312"
SQ   SEQUENCE   244 AA;  27686 MW;  B623844CAF2F008C CRC64;
     MIGGLFLNTL SFVIVSNHPI VNNTANVHHI QQERVIVQQH HVVSARTQNY YPEFSIAVLF
     VSFLALYRST NFKTCVGILM FKILSMTLLG PMLIAYGYYI DGIVTTTVLS LRFAYLAYFW
     YVNSRFEFIL YNTTTLMFVH GRAAPFKRSS HSSIYVTLYG GINYMFVNDL TLHFVDPMLV
     SIAIRGLAHA DLTVVRAVEL LNGDFIYVFS QEPVVGVYNA AFSQAVLNEI DLKEEEGDRT
     YDVS