ID   NS3_BCHK9               Reviewed;         220 AA.
AC   A3EXG7;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   02-JUN-2021, entry version 41.
DE   RecName: Full=Non-structural protein 3;
DE            Short=ns3;
DE   AltName: Full=Accessory protein 3;
GN   ORFNames=3;
OS   Bat coronavirus HKU9 (BtCoV) (BtCoV/HKU9).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Nobecovirus.
OX   NCBI_TaxID=694006;
OH   NCBI_TaxID=9408; Rousettus leschenaultii (Leschenault's rousette).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU9-1;
RX   PubMed=17121802; DOI=10.1128/jvi.02182-06;
RA   Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L.,
RA   Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H.,
RA   Zheng B.-J., Yuen K.-Y.;
RT   "Comparative analysis of twelve genomes of three novel group 2c and group
RT   2d coronaviruses reveals unique group and subgroup features.";
RL   J. Virol. 81:1574-1585(2007).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; EF065513; ABN10912.1; -; Genomic_RNA.
DR   RefSeq; YP_001039972.1; NC_009021.1.
DR   GeneID; 4836013; -.
DR   KEGG; vg:4836013; -.
DR   Proteomes; UP000006576; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   4: Predicted;
KW   Host membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..220
FT                   /note="Non-structural protein 3"
FT                   /id="PRO_0000291329"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        80..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          17..116
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01311"
FT   DOMAIN          120..200
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01312"
SQ   SEQUENCE   220 AA;  24837 MW;  9F7EA8A23A5A2B45 CRC64;
     MNLYNLVRDA LRPSYATVSP SVDEPTVDNN FVALSCYATL SVLLYYLQRV KQPYLSMLFH
     ILFCLSQVCM VIWLIFSANF YVSLFAQCML VVCALGCFLE RTILSIKLRS MAPFMSMADN
     FAIIKTTCNN YVFPVERSSD NLVVLTTSRG IYSNGVFMKG AITVSDNALV VSLFKSHSLL
     LDRVEHGYDY TVFIYINSVI LQNIKPTVSV VNTEFTDVEL