ID   NS3_CVHNL               Reviewed;         225 AA.
AC   Q6Q1S1;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   02-JUN-2021, entry version 48.
DE   RecName: Full=Non-structural protein 3;
DE            Short=Protein 3;
DE            Short=ns3;
DE   AltName: Full=Accessory protein 3a;
GN   ORFNames=3;
OS   Human coronavirus NL63 (HCoV-NL63).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Setracovirus.
OX   NCBI_TaxID=277944;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Amsterdam I;
RX   PubMed=15034574; DOI=10.1038/nm1024;
RA   Van Der Hoek L., Pyrc K., Jebbink M.F., Vermeulen-Oost W., Berkhout R.J.,
RA   Wolthers K.C., Wertheim-Van Dillen P.M., Kaandorp J., Spaargaren J.,
RA   Berkhout B.;
RT   "Identification of a new human coronavirus.";
RL   Nat. Med. 10:368-373(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Amsterdam 057, and Isolate Amsterdam 496;
RX   PubMed=17054987; DOI=10.1016/j.jmb.2006.09.074;
RA   Pyrc K., Dijkman R., Deng L., Jebbink M.F., Ross H.A., Berkhout B.,
RA   van der Hoek L.;
RT   "Mosaic structure of human coronavirus NL63, one thousand years of
RT   evolution.";
RL   J. Mol. Biol. 364:964-973(2006).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; AY567487; AAS58178.1; -; Genomic_RNA.
DR   EMBL; DQ445911; ABE97138.1; -; Genomic_RNA.
DR   EMBL; DQ445912; ABE97131.1; -; Genomic_RNA.
DR   RefSeq; YP_003768.1; NC_005831.2.
DR   GeneID; 2943500; -.
DR   KEGG; vg:2943500; -.
DR   Proteomes; UP000008573; Genome.
DR   Proteomes; UP000115484; Genome.
DR   Proteomes; UP000138462; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR004293; Coronavirus_Orf3b.
DR   Pfam; PF03053; Corona_NS3b; 1.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   4: Predicted;
KW   Host membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..225
FT                   /note="Non-structural protein 3"
FT                   /id="PRO_0000284090"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          35..125
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01311"
FT   DOMAIN          129..208
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01312"
SQ   SEQUENCE   225 AA;  25660 MW;  6497A3DE11A51914 CRC64;
     MPFGGLFQLT LESTINKSVA NLKLPPHDVT VLRDNLKPVT TLSTITAYLL VSLFVTYFAL
     FKPLTARGRV ACFVLKLLTL FVYVPLLVLF GMYLDSFIIF STLLFRFIHV GYYAYLYKNF
     SFVLFNVTKL CFVSGKCWYL EQSFYENRFA AIYGGDHYVV LGGETITFVS FDDLYVAIRG
     SCEKNLQLMR KVDLYNGAVI YIFAEEPVVG IVYSSQLYED VPSIN