ID   NS3_PEDV7               Reviewed;         224 AA.
AC   Q91AV0; Q84705;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   23-FEB-2022, entry version 66.
DE   RecName: Full=Non-structural protein 3;
DE            Short=ns3;
DE   AltName: Full=Accessory protein 3a;
GN   ORFNames=3;
OS   Porcine epidemic diarrhea virus (strain CV777) (PEDV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Pedacovirus.
OX   NCBI_TaxID=229032;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8291230; DOI=10.1006/viro.1994.1058;
RA   Duarte M., Tobler K., Bridgen A., Rasschaert D., Ackermann M., Laude H.;
RT   "Sequence analysis of the porcine epidemic diarrhea virus genome between
RT   the nucleocapsid and spike protein genes reveals a polymorphic ORF.";
RL   Virology 198:466-476(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9782358; DOI=10.1007/978-1-4615-5331-1_101;
RA   Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.;
RT   "Further analysis of the genome of porcine epidemic diarrhea virus.";
RL   Adv. Exp. Med. Biol. 440:781-786(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=11724265; DOI=10.1023/a:1011831902219;
RA   Kocherhans R., Bridgen A., Ackermann M., Tobler K.;
RT   "Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome
RT   sequence.";
RL   Virus Genes 23:137-144(2001).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; Z24733; CAA80855.1; -; Genomic_RNA.
DR   EMBL; AF353511; AAK38657.1; -; Genomic_RNA.
DR   PIR; B49591; B49591.
DR   RefSeq; NP_598311.1; NC_003436.1.
DR   GeneID; 935183; -.
DR   KEGG; vg:935183; -.
DR   Proteomes; UP000008159; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR004293; Coronavirus_Orf3b.
DR   Pfam; PF03053; Corona_NS3b; 1.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   4: Predicted;
KW   Host membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..224
FT                   /note="Non-structural protein 3"
FT                   /id="PRO_0000284091"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          34..124
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01311"
FT   DOMAIN          128..203
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01312"
FT   VARIANT         138
FT                   /note="Missing"
FT   VARIANT         196
FT                   /note="Y -> N"
SQ   SEQUENCE   224 AA;  25280 MW;  AE0092386BF3E47F CRC64;
     MFLGLFQYTI DTVVKDVSKS VNLSLDAVQE LELNVVPIRQ ASNVTGFLFT SVFVYFFALF
     KASSLRRNYI MLAARFAVVF LYCPLLYYCG ALLDATIICC ALIGRLCLVC FYSWRYKNAL
     FIIFNTTTLS FLNGKAAYYD GKSIVILEGG DHYITFGNSF VAFVSNIDLY LAIRGRQEAD
     LHLLRTVELL DGKKLYVFSQ HQIVGITNAA FDSIQLDEYA TISE