ID   NS4A_CVH22              Reviewed;         133 AA.
AC   P19739; Q01160;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   02-JUN-2021, entry version 65.
DE   RecName: Full=Non-structural protein 4a;
DE            Short=ns4a;
DE   AltName: Full=Accessory protein 4a;
DE   Flags: Precursor;
GN   ORFNames=4a;
OS   Human coronavirus 229E (HCoV-229E).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Duvinacovirus.
OX   NCBI_TaxID=11137;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2701946; DOI=10.1093/nar/17.15.6387;
RA   Raabe T., Siddell S.G.;
RT   "Nucleotide sequence of the human coronavirus HCV 229E mRNA 4 and mRNA 5
RT   unique regions.";
RL   Nucleic Acids Res. 17:6387-6387(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate J22;
RX   PubMed=1373555; DOI=10.1016/0168-1702(92)90039-c;
RA   Jouvenne P., Mounir S., Stewart J.N., Richardson C.D., Talbot P.J.;
RT   "Sequence analysis of human coronavirus 229E mRNAs 4 and 5: evidence for
RT   polymorphism and homology with myelin basic protein.";
RL   Virus Res. 22:125-141(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=11369870; DOI=10.1099/0022-1317-82-6-1273;
RA   Thiel V., Herold J., Schelle B., Siddell S.G.;
RT   "Infectious RNA transcribed in vitro from a cDNA copy of the human
RT   coronavirus genome cloned in vaccinia virus.";
RL   J. Gen. Virol. 82:1273-1281(2001).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the coronaviruses NS4a protein family.
CC       {ECO:0000305}.
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DR   EMBL; X15654; CAA33682.1; -; Genomic_RNA.
DR   EMBL; X64942; CAA46113.1; -; Genomic_RNA.
DR   EMBL; AF304460; AAG48593.1; -; Genomic_RNA.
DR   PIR; A34038; MNIHHC.
DR   PIR; S25708; S25708.
DR   RefSeq; NP_073552.1; NC_002645.1.
DR   IntAct; P19739; 115.
DR   PRIDE; P19739; -.
DR   DNASU; 918759; -.
DR   GeneID; 918759; -.
DR   KEGG; vg:918759; -.
DR   Proteomes; UP000006716; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR004293; Coronavirus_Orf3b.
DR   Pfam; PF03053; Corona_NS3b; 1.
DR   PROSITE; PS51966; COV_VIROPORIN_3A_TM; 1.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..133
FT                   /note="Non-structural protein 4a"
FT                   /id="PRO_0000106070"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          34..124
FT                   /note="CoV 3a-like viroporin TM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01311"
FT   VARIANT         41..44
FT                   /note="TFNL -> FSTC (in strain: Isolate J22)"
FT   VARIANT         45..133
FT                   /note="Missing (in strain: Isolate J22)"
SQ   SEQUENCE   133 AA;  15299 MW;  C1995FE1D6C53455 CRC64;
     MALGLFTLQL VSAVNQSLSN AKVSAEVSRQ VIQDVKDGTV TFNLLAYTLM SLFVVYFALF
     KARSHRGRAA LIVFKILILF VYVPLLYWSQ AYIYATLIAV ILLGRFFHTA WHCWLYKTWD
     FIVFNVTTLC YAR