ID   NS4B_CVH22              Reviewed;          88 AA.
AC   P19740; Q01161;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   02-JUN-2021, entry version 64.
DE   RecName: Full=Non-structural protein 4b;
DE            Short=ns4b;
DE   AltName: Full=Accessory protein 4b;
GN   ORFNames=4b;
OS   Human coronavirus 229E (HCoV-229E).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Duvinacovirus.
OX   NCBI_TaxID=11137;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2701946; DOI=10.1093/nar/17.15.6387;
RA   Raabe T., Siddell S.G.;
RT   "Nucleotide sequence of the human coronavirus HCV 229E mRNA 4 and mRNA 5
RT   unique regions.";
RL   Nucleic Acids Res. 17:6387-6387(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate J22;
RX   PubMed=1373555; DOI=10.1016/0168-1702(92)90039-c;
RA   Jouvenne P., Mounir S., Stewart J.N., Richardson C.D., Talbot P.J.;
RT   "Sequence analysis of human coronavirus 229E mRNAs 4 and 5: evidence for
RT   polymorphism and homology with myelin basic protein.";
RL   Virus Res. 22:125-141(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=11369870; DOI=10.1099/0022-1317-82-6-1273;
RA   Thiel V., Herold J., Schelle B., Siddell S.G.;
RT   "Infectious RNA transcribed in vitro from a cDNA copy of the human
RT   coronavirus genome cloned in vaccinia virus.";
RL   J. Gen. Virol. 82:1273-1281(2001).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=9933919; DOI=10.1139/w98-089;
RA   Chagnon F., Lamarre A., Lachance C., Krakowski M., Owens T.,
RA   Laliberte J.F., Talbot P.J.;
RT   "Characterization of the expression and immunogenicity of the ns4b protein
RT   of human coronavirus 229E.";
RL   Can. J. Microbiol. 44:1012-1017(1998).
CC   -!- SIMILARITY: Belongs to the coronaviruses NS4b protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X15654; CAA33683.1; -; Genomic_RNA.
DR   EMBL; X64942; CAA46115.1; -; Genomic_RNA.
DR   EMBL; AF304460; AAG48594.1; -; Genomic_RNA.
DR   PIR; B34038; MNIHH2.
DR   PIR; S25710; S25710.
DR   RefSeq; NP_073553.1; NC_002645.1.
DR   PRIDE; P19740; -.
DR   DNASU; 918760; -.
DR   GeneID; 918760; -.
DR   KEGG; vg:918760; -.
DR   Proteomes; UP000006716; Genome.
DR   InterPro; IPR004293; Coronavirus_Orf3b.
DR   Pfam; PF03053; Corona_NS3b; 1.
DR   PROSITE; PS51967; COV_VIROPORIN_3A_CD; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..88
FT                   /note="Non-structural protein 4b"
FT                   /id="PRO_0000106071"
FT   DOMAIN          1..75
FT                   /note="CoV 3a-like viroporin CD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01312"
FT   VARIANT         1..36
FT                   /note="MQGKCWFLENKALKPFVCFYGGDQFLYIGDRIVSYF -> MSLFVVYFALFK
FT                   ARSHRGRAALIVFKILSYL (in strain: Isolate J22)"
SQ   SEQUENCE   88 AA;  10223 MW;  C25AB329CE83C855 CRC64;
     MQGKCWFLEN KALKPFVCFY GGDQFLYIGD RIVSYFSTND LYVALRGRID KDLSLSRKVE
     LYNGECVYLF CEHPAVGIVN TDFKLEIH