ID   NS4_CVMA5               Reviewed;         128 AA.
AC   P0C5A8;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   23-FEB-2022, entry version 30.
DE   RecName: Full=Non-structural protein 4;
DE            Short=ns4;
DE   AltName: Full=Accessory protein 4;
GN   ORFNames=4;
OS   Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Embecovirus.
OX   NCBI_TaxID=11142;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate C12 mutant;
RX   PubMed=9426441; DOI=10.1006/viro.1997.8877;
RA   Leparc-Goffart I., Hingley S.T., Chua M.M., Jiang X., Lavi E., Weiss S.R.;
RT   "Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is
RT   associated with a Q159L amino acid substitution in the spike protein.";
RL   Virology 239:1-10(1997).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the coronaviruses ns4/ns4.8 protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AF029248; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF029248; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   SMR; P0C5A8; -.
DR   Proteomes; UP000007192; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR005603; Corona_NS4.
DR   Pfam; PF03905; Corona_NS4; 1.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..128
FT                   /note="Non-structural protein 4"
FT                   /id="PRO_0000296315"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   128 AA;  13872 MW;  E42F0DD969BFACE2 CRC64;
     MAVLGPKATL AAVFIGPFIV ACMLGIGLVY LLQLQVQIFH VKDTIRVTGK PATVSYTTST
     PVTPSATTLD GTTYTLIRPT SSYTRVYLGT PRGFDYSTFG PKTLDYVTNL NLILILVVHI
     LLRHCPGI