ID   NS7A_BC279              Reviewed;         122 AA.
AC   Q0Q470;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Protein 7a;
DE   AltName: Full=Accessory protein 7a;
DE   Flags: Precursor;
GN   ORFNames=7a;
OS   Bat coronavirus 279/2005 (BtCoV) (BtCoV/279/2005).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=389167;
OH   NCBI_TaxID=196889; Rhinolophus macrotis (Big-eared horseshoe bat).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=16840328; DOI=10.1128/jvi.00697-06;
RA   Tang X.C., Zhang J.X., Zhang S.Y., Wang P., Fan X.H., Li L.F., Li G.,
RA   Dong B.Q., Liu W., Cheung C.L., Xu K.M., Song W.J., Vijaykrishna D.,
RA   Poon L.L.M., Peiris J.S.M., Smith G.J., Chen H., Guan Y.;
RT   "Prevalence and genetic diversity of coronaviruses in bats from China.";
RL   J. Virol. 80:7481-7490(2006).
CC   -!- FUNCTION: Non-structural protein which is dispensable for virus
CC       replication in cell culture. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the spike glycoprotein, M protein, E protein
CC       and the accessory protein 3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host endoplasmic reticulum
CC       membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC       Host endoplasmic reticulum-Golgi intermediate compartment membrane
CC       {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}. Host
CC       Golgi apparatus membrane {ECO:0000250}; Single-pass membrane protein
CC       {ECO:0000250}.
CC   -!- DOMAIN: The di-lysine motif confers endoplasmic reticulum localization
CC       for type I membrane proteins. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Bat coronavirus 279/2005 is highly similar to SARS-CoV
CC       (SARS-like).
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DR   EMBL; DQ648857; ABG47074.1; -; Genomic_RNA.
DR   SMR; Q0Q470; -.
DR   Proteomes; UP000006573; Genome.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044173; C:host cell endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.1550; -; 1.
DR   InterPro; IPR014888; ORF7a_SARS-CoV-like.
DR   InterPro; IPR044871; ORF7a_SARS-CoV-like_X4e.
DR   InterPro; IPR036495; ORF7a_sf_CoV.
DR   Pfam; PF08779; bCoV_NS7A; 1.
DR   SUPFAM; SSF117066; SSF117066; 1.
DR   PROSITE; PS51919; X4E; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; G0/G1 host cell cycle checkpoint dysregulation by virus;
KW   Host endoplasmic reticulum; Host Golgi apparatus; Host membrane;
KW   Host-virus interaction; Membrane; Modulation of host cell cycle by virus;
KW   Signal; Transmembrane; Transmembrane helix; Virion.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000250"
FT   CHAIN           16..122
FT                   /note="Protein 7a"
FT                   /id="PRO_0000292946"
FT   TOPO_DOM        16..96
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        118..122
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          16..81
FT                   /note="X4e"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01267"
FT   MOTIF           118..122
FT                   /note="Di-lysine motif"
FT                   /evidence="ECO:0000250"
FT   DISULFID        23..58
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01267"
FT   DISULFID        35..67
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01267"
SQ   SEQUENCE   122 AA;  13842 MW;  355C4B908A27C29A CRC64;
     MKIILFLTLI ALASSELYHY QECVRGTTVL LKEPCPSGTY EGNSPFHPLA DNKFALTCIS
     THFAFACADG TRHTYQLRAR SVSPKLFTRQ EEVHQELYSP LFLIVAALVF IILCFTIKRK
     TE