ID   NS7A_BCHK3              Reviewed;         122 AA.
AC   Q3LZX7;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   29-SEP-2021, entry version 68.
DE   RecName: Full=Protein 7a;
DE   AltName: Full=Accessory protein 7a;
DE   Flags: Precursor;
GN   ORFNames=7a;
OS   Bat coronavirus HKU3 (BtCoV) (SARS-like coronavirus HKU3).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=442736;
OH   NCBI_TaxID=89399; Rhinolophus sinicus (Chinese rufous horseshoe bat).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU3-1;
RX   PubMed=16169905; DOI=10.1073/pnas.0506735102;
RA   Lau S.K.P., Woo P.C.Y., Li K.S.M., Huang Y., Tsoi H.-W., Wong B.H.L.,
RA   Wong S.S.Y., Leung S.-Y., Chan K.-H., Yuen K.-Y.;
RT   "Severe acute respiratory syndrome coronavirus-like virus in Chinese
RT   horseshoe bats.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:14040-14045(2005).
CC   -!- FUNCTION: Non-structural protein which is dispensable for virus
CC       replication in cell culture. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the spike glycoprotein, M protein, E protein
CC       and the accessory protein 3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host endoplasmic reticulum
CC       membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC       Host endoplasmic reticulum-Golgi intermediate compartment membrane
CC       {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}. Host
CC       Golgi apparatus membrane {ECO:0000250}; Single-pass membrane protein
CC       {ECO:0000250}.
CC   -!- DOMAIN: The di-lysine motif confers endoplasmic reticulum localization
CC       for type I membrane proteins. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Bat coronavirus HKU3 is highly similar to SARS-CoV
CC       (SARS-like).
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DR   EMBL; DQ022305; AAY88871.1; -; Genomic_RNA.
DR   BMRB; Q3LZX7; -.
DR   SMR; Q3LZX7; -.
DR   Proteomes; UP000007450; Genome.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044173; C:host cell endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.1550; -; 1.
DR   InterPro; IPR014888; ORF7a_SARS-CoV-like.
DR   InterPro; IPR044871; ORF7a_SARS-CoV-like_X4e.
DR   InterPro; IPR036495; ORF7a_sf_CoV.
DR   Pfam; PF08779; bCoV_NS7A; 1.
DR   SUPFAM; SSF117066; SSF117066; 1.
DR   PROSITE; PS51919; X4E; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; G0/G1 host cell cycle checkpoint dysregulation by virus;
KW   Host endoplasmic reticulum; Host Golgi apparatus; Host membrane;
KW   Host-virus interaction; Membrane; Modulation of host cell cycle by virus;
KW   Signal; Transmembrane; Transmembrane helix; Virion.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000250"
FT   CHAIN           16..122
FT                   /note="Protein 7a"
FT                   /id="PRO_0000292947"
FT   TOPO_DOM        16..96
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        118..122
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          16..81
FT                   /note="X4e"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01267"
FT   MOTIF           118..122
FT                   /note="Di-lysine motif"
FT                   /evidence="ECO:0000250"
FT   DISULFID        23..58
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01267"
FT   DISULFID        35..67
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01267"
SQ   SEQUENCE   122 AA;  13884 MW;  EBCD8CCB2333BA67 CRC64;
     MKIILFLTLI ALATCELYHY QECVRGTTVL LKEPCPSGTY EGNSPFHPLA DNKFALTCSS
     THFAFACADG TRHTYQLRAR SVSPKLFIRQ EEVYQELYSP LFLIVAALVF IILCFTIKRK
     TE