ID   NS7_CVPPU               Reviewed;          78 AA.
AC   P04136; Q779N2;
DT   01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   02-JUN-2021, entry version 78.
DE   RecName: Full=Non-structural protein 7;
DE            Short=ns7;
DE   AltName: Full=9 kDa hydrophobic protein;
DE            Short=HP;
DE   AltName: Full=Accessory protein 7;
DE   AltName: Full=X3 protein;
DE   Flags: Precursor;
GN   ORFNames=7;
OS   Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=11151;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2825819; DOI=10.1016/0300-9084(87)90178-7;
RA   Rasschaert D., Gelfi J., Laude H.;
RT   "Enteric coronavirus TGEV: partial sequence of the genomic RNA, its
RT   organization and expression.";
RL   Biochimie 69:591-600(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3008432; DOI=10.1016/0042-6822(86)90102-9;
RA   Kapke P.A., Brian D.A.;
RT   "Sequence analysis of the porcine transmissible gastroenteritis coronavirus
RT   nucleocapsid protein gene.";
RL   Virology 151:41-49(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate PUR46-MAD;
RX   PubMed=10805807; DOI=10.1073/pnas.97.10.5516;
RA   Almazan F., Gonzalez J.M., Penzes Z., Izeta A., Calvo E., Plana-Duran J.,
RA   Enjuanes L.;
RT   "Engineering the largest RNA virus genome as an infectious bacterial
RT   artificial chromosome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:5516-5521(2000).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15240950; DOI=10.1292/jvms.66.717;
RA   Taniguchi T., Taniguchi R., Takahashi A., Hayashidani H., Hanaki K.,
RA   Shirai J., Honda E.;
RT   "Sequence comparison of the ORF 7 region of transmissible gastroenteritis
RT   viruses isolated in Japan.";
RL   J. Vet. Med. Sci. 66:717-719(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Isolate SC-1, and SC-Y;
RA   Ou Y., Yan Q.G., Guo W.Z.;
RT   "Cloning and sequence analysis of Orf7 gene from transmissible
RT   gastroenteritis virus of porcine strain SC-Y.";
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=SC-Y;
RA   Song Z.H., Guo W.Z., Yin H.P., Zeng Z.Y., Ou Y., Han G.Q., Sun Z.Y.;
RT   "Cloning and Seqence Analysis of Genome of Transmissible gastroenteritis
RT   virus SC-Y strain.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=1310191; DOI=10.1016/0042-6822(92)90034-m;
RA   Tung F.Y.T., Abraham S., Sethna M., Hung S.-L., Sethna P.B., Hogue B.A.,
RA   Brian D.A.;
RT   "The 9-kDa hydrophobic protein encoded at the 3' end of the porcine
RT   transmissible gastroenteritis coronavirus genome is membrane-associated.";
RL   Virology 186:676-683(1992).
CC   -!- FUNCTION: May function in the formation of membrane-bound replication
CC       complexes or in the assembly of the virus.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the coronaviruses ns7/ns7a protein family.
CC       {ECO:0000305}.
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DR   EMBL; X06371; CAA29675.1; -; Genomic_RNA.
DR   EMBL; M14878; AAA47916.1; -; Genomic_RNA.
DR   EMBL; AJ271965; CAB91151.1; -; Genomic_RNA.
DR   EMBL; AB115410; BAD34531.1; -; Genomic_RNA.
DR   EMBL; DQ437506; ABD78841.1; -; mRNA.
DR   EMBL; DQ437507; ABD78842.1; -; mRNA.
DR   EMBL; DQ443743; ABD97841.1; -; Genomic_RNA.
DR   PIR; S03937; QQIHPC.
DR   Proteomes; UP000001440; Genome.
DR   Proteomes; UP000158504; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR003449; Corona_7.
DR   Pfam; PF02398; Corona_7; 2.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..78
FT                   /note="Non-structural protein 7"
FT                   /id="PRO_0000106099"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        30
FT                   /note="N -> D (in Ref. 2; AAA47916)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        78
FT                   /note="M -> V (in Ref. 2; AAA47916)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   78 AA;  9130 MW;  7E4F11F864A6B644 CRC64;
     MLVFLHAVFI TVLILLLIGR LQLLERLLLN HSFNLKTVND FNILYRSLAE TRLLKVVLRV
     IFLVLLGFCC YRLLVTLM