ID   NSE4_SCHPO              Reviewed;         300 AA.
AC   Q6BDR8;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Non-structural maintenance of chromosome element 4;
DE            Short=Non-SMC element 4;
DE   AltName: Full=DNA repair protein rad62;
GN   Name=nse4; Synonyms=rad62; ORFNames=SPBC20F10.04c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH THE SMC5-SMC6
RP   COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=15485909; DOI=10.1128/mcb.24.21.9401-9413.2004;
RA   Morikawa H., Morishita T., Kawane S., Iwasaki H., Carr A.M., Shinagawa H.;
RT   "Rad62 protein functionally and physically associates with the smc5/smc6
RT   protein complex and is required for chromosome integrity and recombination
RT   repair in fission yeast.";
RL   Mol. Cell. Biol. 24:9401-9413(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   PARTIAL PROTEIN SEQUENCE, AND INTERACTION WITH NSE1 AND SMC5.
RX   PubMed=15331764; DOI=10.1091/mbc.e04-05-0436;
RA   Pebernard S., McDonald W.H., Pavlova Y., Yates J.R. III, Boddy M.N.;
RT   "Nse1, Nse2, and a novel subunit of the Smc5-Smc6 complex, Nse3, play a
RT   crucial role in meiosis.";
RL   Mol. Biol. Cell 15:4866-4876(2004).
RN   [4]
RP   PARTIAL PROTEIN SEQUENCE, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=16478984; DOI=10.1128/mcb.26.5.1617-1630.2006;
RA   Pebernard S., Wohlschlegel J., McDonald W.H., Yates J.R. III, Boddy M.N.;
RT   "The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.";
RL   Mol. Cell. Biol. 26:1617-1630(2006).
RN   [5]
RP   INTERACTION WITH NSE3.
RX   PubMed=15601840; DOI=10.1128/mcb.25.1.172-184.2005;
RA   Sergeant J., Taylor E., Palecek J., Fousteri M., Andrews E.A., Sweeney S.,
RA   Shinagawa H., Watts F.Z., Lehmann A.R.;
RT   "Composition and architecture of the Schizosaccharomyces pombe Rad18 (Smc5-
RT   6) complex.";
RL   Mol. Cell. Biol. 25:172-184(2005).
CC   -!- FUNCTION: Acts in a DNA repair pathway for removal of UV-induced DNA
CC       damage that is distinct from classical nucleotide excision repair and
CC       in repair of ionizing radiation damage. Functions in homologous
CC       recombination repair of DNA double strand breaks and in recovery of
CC       stalled replication forks. Plays a critical role in meiosis.
CC       {ECO:0000269|PubMed:15485909}.
CC   -!- SUBUNIT: Two subcomplexes smc5-smc6-nse2 and nse1-nse3-nse4 exist.
CC       These subcomplexes are then brought together via a number of
CC       interactions, forming the Smc5-Smc6 complex.
CC   -!- INTERACTION:
CC       Q6BDR8; Q53EK2: nse1; NbExp=5; IntAct=EBI-605484, EBI-605440;
CC       Q6BDR8; Q9Y7U4: nse3; NbExp=7; IntAct=EBI-605484, EBI-605466;
CC       Q6BDR8; O13710: smc5; NbExp=5; IntAct=EBI-605484, EBI-603756;
CC       Q6BDR8; P53692: smc6; NbExp=4; IntAct=EBI-605484, EBI-603745;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15485909}.
CC   -!- SIMILARITY: Belongs to the NSE4 family. {ECO:0000305}.
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DR   EMBL; AB158548; BAD34600.1; -; Genomic_DNA.
DR   EMBL; CU329671; CAH17526.1; -; Genomic_DNA.
DR   RefSeq; NP_001018837.1; NM_001022289.2.
DR   AlphaFoldDB; Q6BDR8; -.
DR   SMR; Q6BDR8; -.
DR   BioGRID; 280406; 17.
DR   IntAct; Q6BDR8; 7.
DR   STRING; 4896.SPBC20F10.04c.1; -.
DR   MaxQB; Q6BDR8; -.
DR   PaxDb; Q6BDR8; -.
DR   EnsemblFungi; SPBC20F10.04c.1; SPBC20F10.04c.1:pep; SPBC20F10.04c.
DR   GeneID; 3361330; -.
DR   KEGG; spo:SPBC20F10.04c; -.
DR   PomBase; SPBC20F10.04c; nse4.
DR   VEuPathDB; FungiDB:SPBC20F10.04c; -.
DR   eggNOG; KOG2866; Eukaryota.
DR   HOGENOM; CLU_041037_1_0_1; -.
DR   InParanoid; Q6BDR8; -.
DR   OMA; TFMGINR; -.
DR   PhylomeDB; Q6BDR8; -.
DR   Reactome; R-SPO-3108214; SUMOylation of DNA damage response and repair proteins.
DR   PRO; PR:Q6BDR8; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0061638; C:CENP-A containing chromatin; IDA:PomBase.
DR   GO; GO:0099115; C:chromosome, subtelomeric region; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005721; C:pericentric heterochromatin; IDA:PomBase.
DR   GO; GO:0033553; C:rDNA heterochromatin; IDA:PomBase.
DR   GO; GO:0030915; C:Smc5-Smc6 complex; IDA:PomBase.
DR   GO; GO:0030674; F:protein-macromolecule adaptor activity; IPI:PomBase.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; EXP:PomBase.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR027786; Nse4/EID.
DR   InterPro; IPR014854; Nse4_C.
DR   InterPro; IPR029225; Nse4_Nse3-bd.
DR   PANTHER; PTHR16140; PTHR16140; 1.
DR   Pfam; PF15412; Nse4-Nse3_bdg; 1.
DR   Pfam; PF08743; Nse4_C; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA damage; DNA recombination; DNA repair;
KW   Meiosis; Nucleus; Reference proteome.
FT   CHAIN           1..300
FT                   /note="Non-structural maintenance of chromosome element 4"
FT                   /id="PRO_0000214099"
SQ   SEQUENCE   300 AA;  34508 MW;  BE3A764555494F89 CRC64;
     MSSIDKRDLR KRYRNLINKV QESRLELVDE ENNNLYETIT TANDLFSSVD APTEATLDAL
     LLTKTVDLAS IKARQLHIGR PKFNIELFTK NIKQFLNYPT SHSNVTRIQE IDTAWSRLGK
     LASNCEKQPA SLNLMVGPLS FRKKERNIQR RERLQKAPNV LTQPTMLNER NITTQENNTT
     KNVLHISRLL QAHQPVNFLK FITNPQSYPQ TVENLFYVSF LFKEGKAALV ENESGILMLE
     TRIPPTDDQV VAGEIRNIQL VLDMTMDLYE NIIKEYNIKE SIIPTRAPVE TSTNSNTWYG