ID   NSE5_SCHPO              Reviewed;         388 AA.
AC   O94668;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Non-structural maintenance of chromosome element 5;
DE            Short=Non-SMC element 5;
GN   Name=nse5; ORFNames=SPBC651.10;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   PARTIAL PROTEIN SEQUENCE, FUNCTION, IDENTIFICATION IN THE SMC5/SMC6
RP   COMPLEX, INTERACTION WITH NSE6, SUBCELLULAR LOCATION, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RX   PubMed=16478984; DOI=10.1128/mcb.26.5.1617-1630.2006;
RA   Pebernard S., Wohlschlegel J., McDonald W.H., Yates J.R. III, Boddy M.N.;
RT   "The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.";
RL   Mol. Cell. Biol. 26:1617-1630(2006).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [4]
RP   INTERACTION WITH RFP1.
RX   PubMed=17502373; DOI=10.1074/jbc.m702652200;
RA   Kosoy A., Calonge T.M., Outwin E.A., O'Connell M.J.;
RT   "Fission yeast Rnf4 homologs are required for DNA repair.";
RL   J. Biol. Chem. 282:20388-20394(2007).
CC   -!- FUNCTION: Acts in a DNA repair pathway for removal of UV-induced DNA
CC       damage that is distinct from classical nucleotide excision repair and
CC       in repair of ionizing radiation damage. Functions in homologous
CC       recombination repair of DNA double strand breaks and in recovery of
CC       stalled replication forks. May prevent formation of excessive Holliday
CC       junctions or assist in their resolution. {ECO:0000269|PubMed:16478984}.
CC   -!- SUBUNIT: Component of the smc5/smc6 complex which consists of two
CC       subcomplexes, smc5-smc6-nse2 and nse1-nse2-nse4. Interacts with nse6
CC       and rfp1. {ECO:0000269|PubMed:16478984, ECO:0000269|PubMed:17502373}.
CC   -!- INTERACTION:
CC       O94668; O13688: nse6; NbExp=7; IntAct=EBI-1150352, EBI-1150368;
CC       O94668; O13710: smc5; NbExp=4; IntAct=EBI-1150352, EBI-603756;
CC       O94668; P53692: smc6; NbExp=2; IntAct=EBI-1150352, EBI-603745;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC       {ECO:0000269|PubMed:16478984, ECO:0000269|PubMed:16823372}. Chromosome
CC       {ECO:0000269|PubMed:16478984}.
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DR   EMBL; CU329671; CAB37606.1; -; Genomic_DNA.
DR   PIR; T40609; T40609.
DR   RefSeq; NP_595508.1; NM_001021418.2.
DR   AlphaFoldDB; O94668; -.
DR   BioGRID; 277427; 48.
DR   IntAct; O94668; 6.
DR   MINT; O94668; -.
DR   STRING; 4896.SPBC651.10.1; -.
DR   iPTMnet; O94668; -.
DR   MaxQB; O94668; -.
DR   PaxDb; O94668; -.
DR   EnsemblFungi; SPBC651.10.1; SPBC651.10.1:pep; SPBC651.10.
DR   GeneID; 2540911; -.
DR   KEGG; spo:SPBC651.10; -.
DR   PomBase; SPBC651.10; nse5.
DR   VEuPathDB; FungiDB:SPBC651.10; -.
DR   HOGENOM; CLU_712051_0_0_1; -.
DR   OMA; CIMKGAG; -.
DR   PRO; PR:O94668; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0030915; C:Smc5-Smc6 complex; IDA:PomBase.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:PomBase.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IC:PomBase.
DR   GO; GO:0048478; P:replication fork protection; TAS:PomBase.
DR   InterPro; IPR014803; DNA_repair_Nse5/Nse6.
DR   Pfam; PF08691; Nse5; 1.
PE   1: Evidence at protein level;
KW   Chromosome; Cytoplasm; Direct protein sequencing; DNA damage;
KW   DNA recombination; DNA repair; Nucleus; Reference proteome.
FT   CHAIN           1..388
FT                   /note="Non-structural maintenance of chromosome element 5"
FT                   /id="PRO_0000116527"
SQ   SEQUENCE   388 AA;  44739 MW;  5995E64A422FBBC9 CRC64;
     MNSALKAIIE LCIEEDLLPK SLDVLEYLLS TTSIPIPACY IRMCITLLVH PEYPTSSNIQ
     ESCNWILQQV VENRSKINFD AWSFENDLSD NDMPKENYLK IFSNQCLFTQ DVDYWDLIAY
     MSSRPPDLER WMSLMDIWLR ILEIDAEENG SALMKKYMGE DLCELQDAIR ICFSCFTLPQ
     QTMESPFATS MSKAPAKTES RGSNSFEGTS RLANLGAKLL CLIWAMLPKG RFFVVHLTDA
     FHFLSKLDER FSKLNAKQQD LFFGNLGASN LRYCLSQYIL MKGIGIGINI RHTRCEFTKQ
     LTQLLPTSLP KNKIRQKLHL RAWVSFLGSA LRESDRNFDK ELFYTTLKQL YTMYQQTELV
     GIDRPMAILK DMLVLLDISK KLSLDKIV