ID   NSE6_SCHPO              Reviewed;         522 AA.
AC   O13688;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Non-structural maintenance of chromosome element 6;
DE            Short=Non-SMC element 6;
DE   AltName: Full=Core protein 1;
GN   Name=nse6; Synonyms=cor1; ORFNames=SPAC11E3.08c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Sawin K.E., Hajibagheri M., Nurse P.;
RT   "A marker for cell middles in fission yeast: evidence for a loss of apical
RT   identity in a polarity mutant.";
RL   Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   PARTIAL PROTEIN SEQUENCE, FUNCTION, IDENTIFICATION IN THE SMC5/SMC6
RP   COMPLEX, INTERACTION WITH NSE5, SUBCELLULAR LOCATION, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RX   PubMed=16478984; DOI=10.1128/mcb.26.5.1617-1630.2006;
RA   Pebernard S., Wohlschlegel J., McDonald W.H., Yates J.R. III, Boddy M.N.;
RT   "The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.";
RL   Mol. Cell. Biol. 26:1617-1630(2006).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Acts in a DNA repair pathway for removal of UV-induced DNA
CC       damage that is distinct from classical nucleotide excision repair and
CC       in repair of ionizing radiation damage. Functions in homologous
CC       recombination repair of DNA double strand breaks and in recovery of
CC       stalled replication forks. May prevent formation of excessive Holliday
CC       junctions or assist in their resolution. {ECO:0000269|PubMed:16478984}.
CC   -!- SUBUNIT: Component of the smc5/smc6 complex which consists of two
CC       subcomplexes, smc5-smc6-nse2 and nse1-nse2-nse4. Interacts with nse5.
CC       {ECO:0000269|PubMed:16478984}.
CC   -!- INTERACTION:
CC       O13688; Q53EK2: nse1; NbExp=2; IntAct=EBI-1150368, EBI-605440;
CC       O13688; Q4PIR3: nse2; NbExp=2; IntAct=EBI-1150368, EBI-605449;
CC       O13688; O94668: nse5; NbExp=7; IntAct=EBI-1150368, EBI-1150352;
CC       O13688; O13710: smc5; NbExp=5; IntAct=EBI-1150368, EBI-603756;
CC       O13688; P53692: smc6; NbExp=4; IntAct=EBI-1150368, EBI-603745;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16478984,
CC       ECO:0000269|PubMed:16823372}. Chromosome {ECO:0000269|PubMed:16478984}.
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DR   EMBL; AJ002494; CAA05501.1; -; mRNA.
DR   EMBL; CU329670; CAB11187.1; -; Genomic_DNA.
DR   PIR; T37536; T37536.
DR   RefSeq; NP_594933.1; NM_001020364.2.
DR   AlphaFoldDB; O13688; -.
DR   BioGRID; 278013; 121.
DR   IntAct; O13688; 7.
DR   MINT; O13688; -.
DR   STRING; 4896.SPAC11E3.08c.1; -.
DR   iPTMnet; O13688; -.
DR   MaxQB; O13688; -.
DR   PaxDb; O13688; -.
DR   EnsemblFungi; SPAC11E3.08c.1; SPAC11E3.08c.1:pep; SPAC11E3.08c.
DR   GeneID; 2541512; -.
DR   KEGG; spo:SPAC11E3.08c; -.
DR   PomBase; SPAC11E3.08c; nse6.
DR   VEuPathDB; FungiDB:SPAC11E3.08c; -.
DR   HOGENOM; CLU_521904_0_0_1; -.
DR   OMA; RCEVKDY; -.
DR   PRO; PR:O13688; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0030915; C:Smc5-Smc6 complex; IDA:PomBase.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:PomBase.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IC:PomBase.
DR   GO; GO:0048478; P:replication fork protection; IGI:PomBase.
DR   InterPro; IPR014803; DNA_repair_Nse5/Nse6.
DR   Pfam; PF08691; Nse5; 1.
PE   1: Evidence at protein level;
KW   Chromosome; Direct protein sequencing; DNA damage; DNA recombination;
KW   DNA repair; Nucleus; Reference proteome.
FT   CHAIN           1..522
FT                   /note="Non-structural maintenance of chromosome element 6"
FT                   /id="PRO_0000079254"
FT   REGION          482..522
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        482..496
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        505..522
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   522 AA;  59899 MW;  36CF241DD74AF0F5 CRC64;
     MNASNNISKF PDLDNSSKLI DHILDSDDSE ELDELPDISS LVPSARAQSR KQYLKNDSSN
     SSTYRWNIDL LSSTATIDDS VAKRRKLAVQ NLLQYDSTQT FQTGDEIDEL IGKSVGSNVL
     NVLRSNPIYD DDLRYEYCSN SKARVPDWNT LKAECLKDND LEFNEGIIPT TFGDLLSAKL
     VPLDIALSIC SLQFFRSLGD TTCSEWIANL EKIFYSYKSS SNNLNQIVRF IFETTADMIG
     IDLAKRQVPI QLERTSASEN LKSNLKIKVI NFLKCCGTLY RFSDDTVRFE MIQDACRILI
     DNQVGSFCKW QFSQFMELPI SLNPDFLISN IHKVSESPRV WVTILSSLSR SCQKFRKKIA
     FTLFVGKQSK NDDSDFSSLC QRLDEISASC NNDYTTLLYQ IRTFGYAVDE KHFKTNERLE
     CLLEKLRKID LTISGSTDHL LLSRCEVKDC IHRLFMVLYY LNTNSAPELE RIIESDLPNN
     NKQKDRYFKD KTSNLSMKEN KSFSAKKVKK GKKKNKRQAY KR