NSF_CAEEL
ID NSF_CAEEL Reviewed; 824 AA.
AC Q94392;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Vesicle-fusing ATPase;
DE EC=3.6.4.6;
DE AltName: Full=N-ethylmaleimide-sensitive fusion protein;
DE Short=NEM-sensitive fusion protein;
DE AltName: Full=Vesicular-fusion protein NSF;
GN Name=nsf-1; ORFNames=H15N14.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion
CC of transport vesicles within the Golgi cisternae. Is also required for
CC transport from the endoplasmic reticulum to the Golgi stack. Seems to
CC function as a fusion protein required for the delivery of cargo
CC proteins to all compartments of the Golgi stack independent of vesicle
CC origin (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.6;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:P18708};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250|UniProtKB:P18708};
CC -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC -!- INTERACTION:
CC Q94392; Q22227: mig-5; NbExp=3; IntAct=EBI-316816, EBI-316403;
CC Q94392; Q27488: pas-2; NbExp=3; IntAct=EBI-316816, EBI-318271;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. {ECO:0000305}.
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DR EMBL; Z96100; CAB09531.1; -; Genomic_DNA.
DR EMBL; Z79698; CAB09531.1; JOINED; Genomic_DNA.
DR PIR; T23096; T23096.
DR RefSeq; NP_001076603.1; NM_001083134.3.
DR AlphaFoldDB; Q94392; -.
DR SMR; Q94392; -.
DR BioGRID; 57330; 6.
DR DIP; DIP-24326N; -.
DR IntAct; Q94392; 4.
DR STRING; 6239.H15N14.2a; -.
DR EPD; Q94392; -.
DR PaxDb; Q94392; -.
DR PeptideAtlas; Q94392; -.
DR PRIDE; Q94392; -.
DR EnsemblMetazoa; H15N14.2a.1; H15N14.2a.1; WBGene00003818.
DR GeneID; 266842; -.
DR KEGG; cel:CELE_H15N14.2; -.
DR UCSC; H15N14.2a; c. elegans.
DR CTD; 266842; -.
DR WormBase; H15N14.2a; CE19925; WBGene00003818; nsf-1.
DR eggNOG; KOG0741; Eukaryota.
DR GeneTree; ENSGT00530000064085; -.
DR InParanoid; Q94392; -.
DR OMA; IQHVKGM; -.
DR OrthoDB; 197562at2759; -.
DR PhylomeDB; Q94392; -.
DR Reactome; R-CEL-204005; COPII-mediated vesicle transport.
DR Reactome; R-CEL-6807878; COPI-mediated anterograde transport.
DR Reactome; R-CEL-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR Reactome; R-CEL-6811438; Intra-Golgi traffic.
DR Reactome; R-CEL-6811440; Retrograde transport at the Trans-Golgi-Network.
DR SignaLink; Q94392; -.
DR PRO; PR:Q94392; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00003818; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q94392; baseline and differential.
DR GO; GO:0005795; C:Golgi stack; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0035494; P:SNARE complex disassembly; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR004201; Cdc48_dom2.
DR InterPro; IPR029067; CDC48_domain_2-like_sf.
DR InterPro; IPR003338; CDC4_N-term_subdom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039812; Vesicle-fus_ATPase.
DR PANTHER; PTHR23078; PTHR23078; 1.
DR Pfam; PF00004; AAA; 2.
DR Pfam; PF17862; AAA_lid_3; 1.
DR Pfam; PF02933; CDC48_2; 1.
DR Pfam; PF02359; CDC48_N; 1.
DR SMART; SM00382; AAA; 2.
DR SMART; SM01073; CDC48_N; 1.
DR SUPFAM; SSF50692; SSF50692; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF54585; SSF54585; 1.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; Hydrolase; Magnesium; Metal-binding;
KW Nucleotide-binding; Protein transport; Reference proteome; Repeat;
KW Transport.
FT CHAIN 1..824
FT /note="Vesicle-fusing ATPase"
FT /id="PRO_0000084565"
FT BINDING 582..587
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P18708"
FT BINDING 622..629
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P18708"
FT BINDING 627
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:P18708"
SQ SEQUENCE 824 AA; 91335 MW; 67232C5115B0B03A CRC64;
MSPVPCLSTC CCFRKIVEYS TMSWFRKSAN DSLLETNHDR IPVAPPREVR APSPRLPPSY
QTSNEKMFRV RKAPSEEHTL ANYAYVNRSD FDDKQIKHVR VNPGPAHHYI FSIRNDGSIK
PGEIAFGVPH RKWAALSLDQ EVRVTPFTFQ QSEYVGSMIL TADFNAKKNV TSEPLNADLM
AREFSIQFGG QAFSKGMQMA FRFEDKEKNK THTLSLVVKS IEGFDIGKAA AAASGASNTD
SSATKPKQIE AGELLPNSVI VFDKEEGSML NLIGKSKGKS AYRSIINPDW DFQQMGIGGL
DTEFSHIFRR AFASRVFPPE FIEQLGMKHV RGILLFGPPG TGKTLMARQI GKMLNAREPK
IVNGPQILDK YVGESESNVR KLFADAEEEW RRCGANSGLH IIIFDEIDAI CKQRGSMAGS
SSVHDTVVNQ LLSKMDGVEQ LNNILVIGMT NRRDMIDEAL LRPGRLEVQM EVSLPDETGR
LQILKIHTAR MREYNKMDPN VDLEDISKRT KNFSGAELEG LVRAAQSSAM NRLVKAGGKA
QADPDAIEKL AINSGDFDYA LENDIKPAFG RSDESLNRFL SRGMIVWGPE VTKILDEGSL
LAATVKNPEN SGFRTVVLAG AAKTGKTSLA AQMAKSSDFP FVKVISPEDT VGFSESAKCM
ALKKAFEDAK RSKLSVLLID NLERLIDYHP VGPRYSNLVI QALLVLLNAP PPAGHRLFVI
ATSSDRMFLR DMGLMDVFGD VIDIPKLTTA GQMMNVIQES NIYSDDQLPM IEQKLASICR
GEGFHGVGIK HLLELIESAR QCEADYRVPT LLNMMEGLAL NLYR
