NSG2_HUMAN
ID NSG2_HUMAN Reviewed; 171 AA.
AC Q9Y328; B2R5Y0; D3DQN0; Q9UHX8;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Neuronal vesicle trafficking-associated protein 2 {ECO:0000312|HGNC:HGNC:24955};
DE AltName: Full=Neuron-specific protein family member 2 {ECO:0000312|HGNC:HGNC:24955};
DE AltName: Full=Protein p19;
DE Short=Hmp19 {ECO:0000303|Ref.1};
GN Name=NSG2 {ECO:0000312|HGNC:HGNC:24955}; Synonyms=CALY3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Jin J., Li G., Hu S., Yuan J., Qiang B.;
RT "Cloning and identifying the HMP19 mRNA.";
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Hypothalamus;
RX PubMed=10931946; DOI=10.1073/pnas.160270997;
RA Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X.,
RA Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W.,
RA Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M., Zhou J., Xu S.-H., Gu J.,
RA Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z.,
RA Chen M.-D., Chen J.-L.;
RT "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis
RT and full-length cDNA cloning.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Amygdala;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC Q9Y328; Q86W74-2: ANKRD46; NbExp=3; IntAct=EBI-7101695, EBI-12109402;
CC Q9Y328; Q9BXN2-6: CLEC7A; NbExp=3; IntAct=EBI-7101695, EBI-11989440;
CC Q9Y328; O14718: RRH; NbExp=3; IntAct=EBI-7101695, EBI-12855824;
CC Q9Y328; Q5BVD1: TTMP; NbExp=3; IntAct=EBI-7101695, EBI-10243654;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q3KR51}; Single-
CC pass type II membrane protein {ECO:0000250|UniProtKB:Q3KR51}. Golgi
CC apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:Q3KR51}.
CC Cell projection, dendrite {ECO:0000250|UniProtKB:Q3KR51}. Endosome
CC membrane {ECO:0000250|UniProtKB:Q3KR51}. Early endosome membrane
CC {ECO:0000250|UniProtKB:Q3KR51}. Late endosome membrane
CC {ECO:0000250|UniProtKB:Q3KR51}. Lysosome lumen
CC {ECO:0000250|UniProtKB:Q3KR51}. Cytoplasmic vesicle membrane
CC {ECO:0000250|UniProtKB:P47759}. Golgi apparatus, Golgi stack membrane
CC {ECO:0000250|UniProtKB:P47759}. Endosome, multivesicular body membrane
CC {ECO:0000250|UniProtKB:P47759}. Note=Endocytosed from the cell surface,
CC thus entered into early endosomes, trafficks to late endosomes and
CC degradates in lysosomes (By similarity). Mainly Golgi stack, but also
CC found in small vacuolar organelles and multivesicular bodies. Found in
CC both stationary and motile endosomes (By similarity).
CC {ECO:0000250|UniProtKB:P47759, ECO:0000250|UniProtKB:Q3KR51}.
CC -!- SIMILARITY: Belongs to the NSG family. {ECO:0000305}.
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DR EMBL; AF133422; AAD30539.1; -; mRNA.
DR EMBL; AF113537; AAF14874.1; -; mRNA.
DR EMBL; AK312359; BAG35277.1; -; mRNA.
DR EMBL; CH471062; EAW61383.1; -; Genomic_DNA.
DR EMBL; CH471062; EAW61384.1; -; Genomic_DNA.
DR EMBL; BC002619; AAH02619.1; -; mRNA.
DR CCDS; CCDS4391.1; -.
DR RefSeq; NP_057064.1; NM_015980.4.
DR AlphaFoldDB; Q9Y328; -.
DR SMR; Q9Y328; -.
DR BioGRID; 119640; 24.
DR IntAct; Q9Y328; 16.
DR MINT; Q9Y328; -.
DR STRING; 9606.ENSP00000307722; -.
DR GlyGen; Q9Y328; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9Y328; -.
DR PhosphoSitePlus; Q9Y328; -.
DR BioMuta; NSG2; -.
DR DMDM; 21263843; -.
DR MassIVE; Q9Y328; -.
DR PaxDb; Q9Y328; -.
DR PeptideAtlas; Q9Y328; -.
DR PRIDE; Q9Y328; -.
DR ProteomicsDB; 85965; -.
DR Antibodypedia; 45952; 80 antibodies from 20 providers.
DR DNASU; 51617; -.
DR Ensembl; ENST00000303177.8; ENSP00000307722.3; ENSG00000170091.11.
DR Ensembl; ENST00000519717.1; ENSP00000431119.1; ENSG00000170091.11.
DR GeneID; 51617; -.
DR KEGG; hsa:51617; -.
DR MANE-Select; ENST00000303177.8; ENSP00000307722.3; NM_015980.5; NP_057064.1.
DR UCSC; uc003mcx.4; human.
DR CTD; 51617; -.
DR DisGeNET; 51617; -.
DR GeneCards; NSG2; -.
DR HGNC; HGNC:24955; NSG2.
DR HPA; ENSG00000170091; Tissue enriched (brain).
DR MIM; 616752; gene.
DR neXtProt; NX_Q9Y328; -.
DR OpenTargets; ENSG00000170091; -.
DR VEuPathDB; HostDB:ENSG00000170091; -.
DR eggNOG; ENOG502QRFC; Eukaryota.
DR GeneTree; ENSGT00390000000483; -.
DR InParanoid; Q9Y328; -.
DR OMA; QHDAKPP; -.
DR OrthoDB; 1315835at2759; -.
DR PhylomeDB; Q9Y328; -.
DR PathwayCommons; Q9Y328; -.
DR SignaLink; Q9Y328; -.
DR BioGRID-ORCS; 51617; 3 hits in 955 CRISPR screens.
DR GenomeRNAi; 51617; -.
DR Pharos; Q9Y328; Tbio.
DR PRO; PR:Q9Y328; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q9Y328; protein.
DR Bgee; ENSG00000170091; Expressed in cortical plate and 134 other tissues.
DR ExpressionAtlas; Q9Y328; baseline and differential.
DR Genevisible; Q9Y328; HS.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; ISS:UniProtKB.
DR GO; GO:0030425; C:dendrite; ISS:UniProtKB.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:HPA.
DR GO; GO:1990674; C:Golgi cis cisterna membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005770; C:late endosome; ISS:UniProtKB.
DR GO; GO:0043202; C:lysosomal lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0032585; C:multivesicular body membrane; ISS:UniProtKB.
DR GO; GO:0032588; C:trans-Golgi network membrane; ISS:UniProtKB.
DR GO; GO:0032051; F:clathrin light chain binding; IBA:GO_Central.
DR GO; GO:0048268; P:clathrin coat assembly; IBA:GO_Central.
DR GO; GO:0007212; P:dopamine receptor signaling pathway; IEA:InterPro.
DR GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR InterPro; IPR009431; NSG.
DR PANTHER; PTHR28546; PTHR28546; 1.
DR Pfam; PF06387; Calcyon; 1.
DR PIRSF; PIRSF002383; Calcyon; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cytoplasmic vesicle; Endosome; Golgi apparatus; Lysosome;
KW Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..171
FT /note="Neuronal vesicle trafficking-associated protein 2"
FT /id="PRO_0000164366"
FT TOPO_DOM 1..71
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..171
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 152
FT /note="P -> R (in Ref. 2; AAF14874)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 171 AA; 19085 MW; F5E69DEA61D0FFEC CRC64;
MVKLNSNPSE KGTKPPSVED GFQTVPLITP LEVNHLQLPA PEKVIVKTRT EYQPEQKNKG
KFRVPKIAEF TVTILVSLAL AFLACIVFLV VYKAFTYDHS CPEGFVYKHK RCIPASLDAY
YSSQDPNSRS RFYTVISHYS VAKQSTARAI GPWLSAAAVI HEPKPPKTQG H
