NSK1_SCHPO
ID NSK1_SCHPO Reviewed; 462 AA.
AC Q7LL22;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Kinetochore protein nsk1;
DE AltName: Full=Nucleolus spindle kinetochore protein 1;
GN Name=nsk1; ORFNames=SPAC3G9.01;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH DLC1.
RX PubMed=22065639; DOI=10.1083/jcb.201105074;
RA Chen J.S., Lu L.X., Ohi M.D., Creamer K.M., English C., Partridge J.F.,
RA Ohi R., Gould K.L.;
RT "Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone
RT chromosome segregation.";
RL J. Cell Biol. 195:583-593(2011).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21965289; DOI=10.1091/mbc.e11-07-0608;
RA Buttrick G.J., Meadows J.C., Lancaster T.C., Vanoosthuyse V.,
RA Shepperd L.A., Hoe K.L., Kim D.U., Park H.O., Hardwick K.G., Millar J.B.;
RT "Nsk1 ensures accurate chromosome segregation by promoting association of
RT kinetochores to spindle poles during anaphase B.";
RL Mol. Biol. Cell 22:4486-4502(2011).
CC -!- FUNCTION: Ensures chromosome alignment and accurate chromosome
CC segregation during mitosis. Promotes proper kinetochore-microtubule (k-
CC MT) interactions during anaphase B. The phosphorylation status of nsk1
CC affects the proper k-MT coupling, ensuring that it interacts stably
CC only at the correct time during mitosis. {ECO:0000269|PubMed:21965289,
CC ECO:0000269|PubMed:22065639}.
CC -!- SUBUNIT: Interacts with dlc1. The dlc1-nsk1 complex seems to
CC oligomerize in chain-like structures. Also binds directly to spindle
CC microtubules. {ECO:0000269|PubMed:22065639}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:21965289}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:21965289,
CC ECO:0000269|PubMed:22065639}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:21965289, ECO:0000269|PubMed:22065639}.
CC Note=Undergoes cell cycle-dependent localization changes, nucleolar
CC during interphase, distributed in the nucleoplasm during metaphase, and
CC increased at kinetochores and the spindle during anaphase.
CC {ECO:0000269|PubMed:21965289}.
CC -!- PTM: Phosphorylated by cdk1 at prometaphase arrest. Phosphorylation
CC prevents nsk1 kinetochore and spindle targeting. Dephosphorylated by
CC clp1 at anaphase onset controls its relocalization.
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DR EMBL; CU329670; CAB38630.1; -; Genomic_DNA.
DR RefSeq; NP_001018258.1; NM_001019512.2.
DR AlphaFoldDB; Q7LL22; -.
DR SMR; Q7LL22; -.
DR BioGRID; 280546; 39.
DR STRING; 4896.SPAC3G9.01.1; -.
DR iPTMnet; Q7LL22; -.
DR MaxQB; Q7LL22; -.
DR PaxDb; Q7LL22; -.
DR PRIDE; Q7LL22; -.
DR EnsemblFungi; SPAC3G9.01.1; SPAC3G9.01.1:pep; SPAC3G9.01.
DR GeneID; 3361470; -.
DR KEGG; spo:SPAC3G9.01; -.
DR PomBase; SPAC3G9.01; nsk1.
DR VEuPathDB; FungiDB:SPAC3G9.01; -.
DR HOGENOM; CLU_592053_0_0_1; -.
DR PhylomeDB; Q7LL22; -.
DR PRO; PR:Q7LL22; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0061497; C:inner plaque of mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0072686; C:mitotic spindle; IDA:PomBase.
DR GO; GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0044816; C:Nsk1-Dlc1 complex; IDA:PomBase.
DR GO; GO:0005730; C:nucleolus; IDA:PomBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0000940; C:outer kinetochore; IDA:PomBase.
DR GO; GO:0051010; F:microtubule plus-end binding; EXP:PomBase.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IMP:PomBase.
DR GO; GO:0072766; P:centromere clustering at the mitotic interphase nuclear envelope; IMP:PomBase.
PE 1: Evidence at protein level;
KW Centromere; Chromosome; Cytoplasm; Cytoskeleton; Kinetochore; Nucleus;
KW Reference proteome.
FT CHAIN 1..462
FT /note="Kinetochore protein nsk1"
FT /id="PRO_0000304077"
FT REGION 104..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 320..462
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 104..119
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 196..240
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 320..335
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 350..371
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 403..446
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 462 AA; 51571 MW; 4566E668AC5700FF CRC64;
MAYKLSLSAV EPLLIFPAAP FSTKKEKSTF QNNLISSDVF SSALKPLSPL KSLVADFSTH
SNPSKDNQLI SPPKINHREF LNEEKESDTQ TRYLKQLINI CNSPSKNHET SLSPSKSTID
NNERKLDNEI DNYKHDVKYS PYKGQGKTSN PSQGTTKCPG IFEEDNFFSV SPKRRKNLFE
KYGKTDLGKP ARVPSPKKSL SSTIKSPPSR VKLPTSILSK SPPLKVPNKN RSSTFSPLRT
PTSSSKTFVI VDHSTPSPPS IRTKLEAFAP NSTFATQKRL RRLATLESSP ALRSTLKSLQ
SKSSPVKLLK LKEEKAKIKN QLFKSEEEKD PVGKQKLPLE SSLSPLDHSS AEKEMQKAPA
KNKRRRTGSL ETGLYPKESP TPSKKRSKRV LWSLKHIVSP GNREKHSLNS TPESIMKKDT
KWPQNLAKNN INSEPNTPTK SNIDTGKAHS ARAHKTRKNI QS
