NSL1_YEAST
ID NSL1_YEAST Reviewed; 216 AA.
AC Q12143; D6W3D7; Q6B152;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Kinetochore-associated protein NSL1;
GN Name=NSL1; OrderedLocusNames=YPL233W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12455957; DOI=10.1128/ec.1.2.229-240.2002;
RA Euskirchen G.M.;
RT "Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a new group of proteins important for
RT chromosome segregation in Saccharomyces cerevisiae.";
RL Eukaryot. Cell 1:229-240(2002).
RN [5]
RP IDENTIFICATION IN THE MIND COMPLEX.
RX PubMed=14602074; DOI=10.1016/s1534-5807(03)00322-8;
RA Pinsky B.A., Tatsutani S.Y., Collins K.A., Biggins S.;
RT "An Mtw1 complex promotes kinetochore biorientation that is monitored by
RT the Ipl1/Aurora protein kinase.";
RL Dev. Cell 5:735-745(2003).
RN [6]
RP FUNCTION, IDENTIFICATION IN THE MIND COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=14657030; DOI=10.1093/emboj/cdg636;
RA Scharfenberger M., Ortiz J., Grau N., Janke C., Schiebel E., Lechner J.;
RT "Nsl1p is essential for the establishment of bipolarity and the
RT localization of the Dam-Duo complex.";
RL EMBO J. 22:6584-6597(2003).
RN [7]
RP IDENTIFICATION IN THE MIND COMPLEX, AND FUNCTION OF THE MIND COMPLEX.
RX PubMed=14633972; DOI=10.1101/gad.1144403;
RA De Wulf P., McAinsh A.D., Sorger P.K.;
RT "Hierarchical assembly of the budding yeast kinetochore from multiple
RT subcomplexes.";
RL Genes Dev. 17:2902-2921(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH DSN1.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Acts as essential component of the kinetochore MIND complex,
CC which is required for the spindle checkpoint and kinetochore integrity.
CC MIND plays a role in establishing a bipolar spindle-kinetochore
CC interaction by joining kinetochore subunits contacting DNA to those
CC contacting microtubules. NSL1 facilitates the attachment of two of the
CC DASH complex components, DAD2 and SPC19, to the kinetochore in a
CC microtubule-dependent manner. {ECO:0000269|PubMed:12455957,
CC ECO:0000269|PubMed:14633972, ECO:0000269|PubMed:14657030}.
CC -!- SUBUNIT: Component of the MIND kinetochore complex, which is composed
CC of at least MTW1, NNF1, NSL1 and DSN1. Interacts with DSN1.
CC {ECO:0000269|PubMed:14602074, ECO:0000269|PubMed:14633972,
CC ECO:0000269|PubMed:14657030, ECO:0000269|PubMed:14690591}.
CC -!- INTERACTION:
CC Q12143; P40568: DSN1; NbExp=13; IntAct=EBI-33666, EBI-25398;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12455957,
CC ECO:0000269|PubMed:14657030}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:12455957, ECO:0000269|PubMed:14657030}.
CC Note=Associated with the kinetochore (PubMed:12455957,
CC PubMed:14657030).
CC -!- MISCELLANEOUS: Present with 3710 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z73589; CAA97950.1; -; Genomic_DNA.
DR EMBL; X94561; CAA64254.1; -; Genomic_DNA.
DR EMBL; AY693228; AAT93247.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11203.1; -; Genomic_DNA.
DR PIR; S61701; S61701.
DR RefSeq; NP_015091.1; NM_001184047.1.
DR AlphaFoldDB; Q12143; -.
DR SMR; Q12143; -.
DR BioGRID; 35929; 371.
DR ComplexPortal; CPX-1186; Nuclear MIS12/MIND complex.
DR DIP; DIP-5393N; -.
DR IntAct; Q12143; 23.
DR MINT; Q12143; -.
DR STRING; 4932.YPL233W; -.
DR iPTMnet; Q12143; -.
DR MaxQB; Q12143; -.
DR PaxDb; Q12143; -.
DR PRIDE; Q12143; -.
DR EnsemblFungi; YPL233W_mRNA; YPL233W; YPL233W.
DR GeneID; 855843; -.
DR KEGG; sce:YPL233W; -.
DR SGD; S000006154; NSL1.
DR VEuPathDB; FungiDB:YPL233W; -.
DR eggNOG; ENOG502SA1D; Eukaryota.
DR HOGENOM; CLU_100671_0_0_1; -.
DR InParanoid; Q12143; -.
DR OMA; QEWEDQT; -.
DR BioCyc; YEAST:G3O-34120-MON; -.
DR PRO; PR:Q12143; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12143; protein.
DR GO; GO:0000776; C:kinetochore; IDA:SGD.
DR GO; GO:0000444; C:MIS12/MIND type complex; IBA:GO_Central.
DR GO; GO:0000818; C:nuclear MIS12/MIND complex; IDA:SGD.
DR GO; GO:0000922; C:spindle pole; IDA:SGD.
DR GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IC:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IDA:SGD.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR InterPro; IPR013950; Mis14/Nsl1.
DR PANTHER; PTHR31749; PTHR31749; 1.
DR Pfam; PF08641; Mis14; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Centromere; Chromosome;
KW Kinetochore; Mitosis; Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..216
FT /note="Kinetochore-associated protein NSL1"
FT /id="PRO_0000057965"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CONFLICT 212
FT /note="F -> L (in Ref. 3; AAT93247)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 216 AA; 25416 MW; BE627C405B4831A5 CRC64;
MSQGQSKKLD VTVEQLRSIY HQFHDILEEK TDLHLPKKEY DDDAVRREVQ IQLQEFLLSA
MTMASKSLEV VNADTVGKTV KQLIMESQEK YMEPFDLDLN EQVRKMYQEW EDETVKVAQL
RQTGPAKINE VYNNSKDEYL AQLDGRIGVL QARMMQQQSA DHDDSTDDAD DHINWEHIKQ
DYVASLNELY QTQQDLPKVR YNVEKVKRLM DFLEED
