ID   NSMA_CAEEL              Reviewed;         434 AA.
AC   O45870;
DT   11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2003, sequence version 2.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Putative neutral sphingomyelinase;
DE            EC=3.1.4.12;
GN   ORFNames=T27F6.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a sphingomyelin + H2O = an N-acylsphing-4-enine + H(+) +
CC         phosphocholine; Xref=Rhea:RHEA:19253, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17636, ChEBI:CHEBI:52639,
CC         ChEBI:CHEBI:295975; EC=3.1.4.12;
CC   -!- PATHWAY: Lipid metabolism; sphingolipid metabolism.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the neutral sphingomyelinase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z82060; CAB04885.2; -; Genomic_DNA.
DR   PIR; T25386; T25386.
DR   RefSeq; NP_493169.2; NM_060768.4.
DR   AlphaFoldDB; O45870; -.
DR   SMR; O45870; -.
DR   BioGRID; 38515; 10.
DR   STRING; 6239.T27F6.6; -.
DR   iPTMnet; O45870; -.
DR   EPD; O45870; -.
DR   PaxDb; O45870; -.
DR   PeptideAtlas; O45870; -.
DR   PRIDE; O45870; -.
DR   EnsemblMetazoa; T27F6.6.1; T27F6.6.1; WBGene00012105.
DR   EnsemblMetazoa; T27F6.6.2; T27F6.6.2; WBGene00012105.
DR   GeneID; 173118; -.
DR   KEGG; cel:CELE_T27F6.6; -.
DR   UCSC; T27F6.6; c. elegans.
DR   CTD; 173118; -.
DR   WormBase; T27F6.6; CE34214; WBGene00012105; -.
DR   eggNOG; KOG3873; Eukaryota.
DR   GeneTree; ENSGT00390000009166; -.
DR   HOGENOM; CLU_034001_1_0_1; -.
DR   InParanoid; O45870; -.
DR   OMA; IEVNCQV; -.
DR   OrthoDB; 589366at2759; -.
DR   PhylomeDB; O45870; -.
DR   Reactome; R-CEL-1660662; Glycosphingolipid metabolism.
DR   UniPathway; UPA00222; -.
DR   PRO; PR:O45870; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00012105; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005901; C:caveola; IBA:GO_Central.
DR   GO; GO:0071944; C:cell periphery; IBA:GO_Central.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004620; F:phospholipase activity; IBA:GO_Central.
DR   GO; GO:0004767; F:sphingomyelin phosphodiesterase activity; IBA:GO_Central.
DR   GO; GO:0046513; P:ceramide biosynthetic process; IBA:GO_Central.
DR   GO; GO:0030149; P:sphingolipid catabolic process; IBA:GO_Central.
DR   GO; GO:0006684; P:sphingomyelin metabolic process; IBA:GO_Central.
DR   Gene3D; 3.60.10.10; -; 1.
DR   InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR   InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR   InterPro; IPR038772; SMPD2-like.
DR   PANTHER; PTHR12393; PTHR12393; 1.
DR   Pfam; PF03372; Exo_endo_phos; 1.
DR   SUPFAM; SSF56219; SSF56219; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Lipid metabolism; Magnesium; Membrane; Metal-binding;
KW   Reference proteome; Sphingolipid metabolism; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..434
FT                   /note="Putative neutral sphingomyelinase"
FT                   /id="PRO_0000075689"
FT   TRANSMEM        366..388
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        318
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         83
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   SITE            215
FT                   /note="Important for substrate recognition"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   434 AA;  49864 MW;  5AE68CAE361977FB CRC64;
     MEPSSEQESQ ESQKVDMEPV FRRESFAALR RNNVAARQLR VVTLNAWCLP QPWPIGSTDR
     VHRLNKIGQY MIDELYDIVG LQELWSYYDF VRLSEQVSSV YPYFHYFHSG FTGSGVCVFS
     RHPIVSTLTN RYSLNGFAHH IHRGDWFGGK VVGLTEIEID GDLRVNFYTT HLHAEYDREN
     DLYLPHRTAQ AFELAQFVRH TARGADVVIV TGDLNMEPCD LGFRLILSHA KLFDAWRMSH
     EVENEDSEGE LLKFRGIAKG GTCDRPDNCY TKRALKNVDD SKRIDYMLFK SGRCNVKLEE
     CEITLNQIPG EDLNYSDHVG LRARFTIDDR FRHEKSVNTW EPNRPLLIEA IGLVAGGERR
     ARTDRIFFFI LAVICLILIL GSLFFEVFPM GFAVLRFALT VVGVFFVWQG LIGLTLERKA
     LKAAKQAIQQ ILNN