ID   NSP1_LOTJA              Reviewed;         542 AA.
AC   A1DQP9; A1DR75;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Protein NODULATION SIGNALING PATHWAY 1 {ECO:0000303|PubMed:17071642};
DE            Short=LsNSP1 {ECO:0000303|PubMed:17071642};
GN   Name=NSP1 {ECO:0000303|PubMed:17071642};
OS   Lotus japonicus (Lotus corniculatus var. japonicus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX   NCBI_TaxID=34305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION,
RP   AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Gifu / B-129;
RX   PubMed=17071642; DOI=10.1104/pp.106.089508;
RA   Heckmann A.B., Lombardo F., Miwa H., Perry J.A., Bunnewell S., Parniske M.,
RA   Wang T.L., Downie J.A.;
RT   "Lotus japonicus nodulation requires two GRAS domain regulators, one of
RT   which is functionally conserved in a non-legume.";
RL   Plant Physiol. 142:1739-1750(2006).
RN   [2]
RP   FUNCTION.
RX   PubMed=20719766; DOI=10.1093/pcp/pcq124;
RA   Yokota K., Soyano T., Kouchi H., Hayashi M.;
RT   "Function of GRAS proteins in root nodule symbiosis is retained in homologs
RT   of a non-legume, rice.";
RL   Plant Cell Physiol. 51:1436-1442(2010).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=23335614; DOI=10.1093/pcp/pct008;
RA   Murakami Y., Yokoyama H., Fukui R., Kawaguchi M.;
RT   "Down-regulation of NSP2 expression in developmentally young regions of
RT   Lotus japonicus roots in response to rhizobial inoculation.";
RL   Plant Cell Physiol. 54:518-527(2013).
RN   [4]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23926062; DOI=10.1093/pcp/pct114;
RA   Takeda N., Tsuzuki S., Suzaki T., Parniske M., Kawaguchi M.;
RT   "CERBERUS and NSP1 of Lotus japonicus are common symbiosis genes that
RT   modulate arbuscular mycorrhiza development.";
RL   Plant Cell Physiol. 54:1711-1723(2013).
RN   [5]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24329948; DOI=10.1111/tpj.12374;
RA   Hayashi T., Shimoda Y., Sato S., Tabata S., Imaizumi-Anraku H., Hayashi M.;
RT   "Rhizobial infection does not require cortical expression of upstream
RT   common symbiosis genes responsible for the induction of Ca(2+) spiking.";
RL   Plant J. 77:146-159(2014).
CC   -!- FUNCTION: Transcriptional regulator essential for Nod-factor-induced
CC       gene expression (PubMed:17071642) (Probable). Acts downstream of
CC       calcium spiking and a calcium/calmodulin-dependent protein kinase
CC       required for activation of early nodulation gene expression
CC       (PubMed:17071642, PubMed:24329948). Acts as a common symbiosis gene
CC       that positively contributes to the early steps of the arbuscular
CC       mycorrhizal fungus and rhizobial infection processes in roots
CC       (PubMed:23926062). Transcription factor involved in the positive
CC       regulation of the beta-carotene isomerase D27, which participates in a
CC       pathway leading to biosynthesis of strigolactones in roots
CC       (PubMed:23926062). {ECO:0000269|PubMed:17071642,
CC       ECO:0000269|PubMed:23926062, ECO:0000269|PubMed:24329948,
CC       ECO:0000305|PubMed:20719766, ECO:0000305|PubMed:23335614}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in roots.
CC       {ECO:0000269|PubMed:17071642}.
CC   -!- INDUCTION: Induced in roots after inoculation with Mesorhizobium loti
CC       (PubMed:17071642, PubMed:23335614, PubMed:24329948). Induced in roots
CC       by infection with arbuscular mycorrhizal fungus Rhizophagus irregularis
CC       (PubMed:23926062). {ECO:0000269|PubMed:17071642,
CC       ECO:0000269|PubMed:23335614, ECO:0000269|PubMed:23926062,
CC       ECO:0000269|PubMed:24329948}.
CC   -!- DISRUPTION PHENOTYPE: Roots of knockout plants form few aborted and
CC       non-functional nodules under nitrogen limitation when inoculated with
CC       Mesorhizobium loti (PubMed:17071642, PubMed:23926062, PubMed:24329948).
CC       Low level of arbuscular mycorrhizal (AM) colonization in roots
CC       inoculated with the AM fungus Rhizophagus irregularis
CC       (PubMed:23926062). {ECO:0000269|PubMed:17071642,
CC       ECO:0000269|PubMed:23926062, ECO:0000269|PubMed:24329948}.
CC   -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000305}.
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DR   EMBL; EF012819; ABK35066.1; -; Genomic_DNA.
DR   EMBL; EF017372; ABK35067.1; -; Genomic_DNA.
DR   AlphaFoldDB; A1DQP9; -.
DR   SMR; A1DQP9; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR   InterPro; IPR030015; SCL29/NSP1.
DR   InterPro; IPR005202; TF_GRAS.
DR   PANTHER; PTHR31636; PTHR31636; 1.
DR   PANTHER; PTHR31636:SF243; PTHR31636:SF243; 1.
DR   Pfam; PF03514; GRAS; 1.
DR   PROSITE; PS50985; GRAS; 1.
PE   2: Evidence at transcript level;
KW   Nodulation; Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..542
FT                   /note="Protein NODULATION SIGNALING PATHWAY 1"
FT                   /id="PRO_0000448272"
FT   DOMAIN          145..532
FT                   /note="GRAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          73..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          152..214
FT                   /note="Leucine repeat I (LRI)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          233..332
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          333..357
FT                   /note="Leucine repeat II (LRII)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          367..452
FT                   /note="PFYRE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          455..532
FT                   /note="SAW"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   MOTIF           269..273
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   COMPBIAS        73..93
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        114..133
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   542 AA;  59839 MW;  D88D9B3EC11ABF1C CRC64;
     MTMEPNSTTS DHILDWLEGS VSFFPSFLDE PCNNSGYIQE YHLWDQYQTD ANTGSSPNAT
     NSTTATIVAT SATSTTSLEP CGSNNNQPLS DLPKKRNATD ESSLKPPQNK NKRIKTRPMN
     EPENGDAVRK NKKGGAKANG SNCNSGNSKE GRWAEQLLNP CAAAIAGGNV NRVQHLLYVL
     HELASPTGDP NHRLAAHGLR ALTHHLSSSS SSPTSSGTIT FASTEPRFFQ KSLLKFYEVS
     PWFSFPNNIA NASILQVLAE EANITSRTLH ILDIGVSHGV QWPTLLDALS RRSGGPPSVV
     RLTVVTAEND QNMETPFSKA PPGYNYYPRL LGYAQSININ LQINRIENHS LQTLNAQSIS
     ASPDEILIVC AQFRLHHLNH NSPDERSEFL KVLRNMEPRG VILSENNTEC CCSGCGNFAA
     GFTRRVEYLW RFLDSTSSAF KGRESDERRV MEGEAAKALT NQREMNEEKE KWCGRMKEAG
     FAGEVFGEDA VDGGRALLRK YDSNWEMKVE EKNTSVGLWW KGQPVSFCSL WKLDGNDQGG
     TS