NSP1_MEDTR
ID NSP1_MEDTR Reviewed; 554 AA.
AC Q4VYC8; G7L7I4; Q4VYC5; Q4VYC6;
DT 02-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Protein NODULATION SIGNALING PATHWAY 1 {ECO:0000303|PubMed:15961669};
GN Name=NSP1 {ECO:0000303|PubMed:15961669};
GN OrderedLocusNames=MTR_8g020840 {ECO:0000312|EMBL:AET01770.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF
RP 240-ARG--GLU-554 AND 488-TRP--GLU-554, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=15961669; DOI=10.1126/science.1111025;
RA Smit P., Raedts J., Portyanko V., Debelle F., Gough C., Bisseling T.,
RA Geurts R.;
RT "NSP1 of the GRAS protein family is essential for rhizobial Nod factor-
RT induced transcription.";
RL Science 308:1789-1791(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [4]
RP FUNCTION.
RX PubMed=23077241; DOI=10.1104/pp.112.203190;
RA Cerri M.R., Frances L., Laloum T., Auriac M.C., Niebel A., Oldroyd G.E.,
RA Barker D.G., Fournier J., de Carvalho-Niebel F.;
RT "Medicago truncatula ERN transcription factors: regulatory interplay with
RT NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial
RT infection.";
RL Plant Physiol. 160:2155-2172(2012).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=22039214; DOI=10.1105/tpc.111.089771;
RA Liu W., Kohlen W., Lillo A., Op den Camp R., Ivanov S., Hartog M.,
RA Limpens E., Jamil M., Smaczniak C., Kaufmann K., Yang W.C., Hooiveld G.J.,
RA Charnikhova T., Bouwmeester H.J., Bisseling T., Geurts R.;
RT "Strigolactone biosynthesis in Medicago truncatula and rice requires the
RT symbiotic GRAS-type transcription factors NSP1 and NSP2.";
RL Plant Cell 23:3853-3865(2011).
RN [6]
RP FUNCTION.
RX PubMed=26503135; DOI=10.1186/s12870-015-0651-x;
RA van Zeijl A., Liu W., Xiao T.T., Kohlen W., Yang W.C., Bisseling T.,
RA Geurts R.;
RT "The strigolactone biosynthesis gene DWARF27 is co-opted in rhizobium
RT symbiosis.";
RL BMC Plant Biol. 15:260-260(2015).
CC -!- FUNCTION: Transcriptional regulator essential for Nod-factor-induced
CC gene expression (PubMed:15961669). Acts downstream of calcium spiking
CC (PubMed:15961669). May be a target of DMI3, a calcium/calmodulin-
CC dependent protein kinase (CCaMK) (PubMed:15961669). Is essential for
CC Nod factor-elicited expression of ERN1 (PubMed:23077241). Transcription
CC factor involved in the control of strigolactone biosynthesis in roots
CC through the activation of the beta-carotene isomerase D27, which
CC participates in a pathway leading to biosynthesis of strigolactones
CC (PubMed:22039214, PubMed:26503135). {ECO:0000269|PubMed:15961669,
CC ECO:0000269|PubMed:22039214, ECO:0000269|PubMed:23077241,
CC ECO:0000269|PubMed:26503135}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in epidermal and cortical root cells.
CC {ECO:0000269|PubMed:15961669}.
CC -!- INDUCTION: Not induced after treatment with Sinorhizobium meliloti.
CC {ECO:0000269|PubMed:15961669}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000305}.
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DR EMBL; AJ972478; CAJ00010.1; -; Genomic_DNA.
DR EMBL; AJ972473; CAJ00005.1; -; Genomic_DNA.
DR EMBL; AJ972480; CAJ00012.1; -; Genomic_DNA.
DR EMBL; AJ972481; CAJ00013.1; -; Genomic_DNA.
DR EMBL; CM001224; AET01770.1; -; Genomic_DNA.
DR RefSeq; XP_003627294.1; XM_003627246.2.
DR AlphaFoldDB; Q4VYC8; -.
DR SMR; Q4VYC8; -.
DR STRING; 3880.AET01770; -.
DR PRIDE; Q4VYC8; -.
DR EnsemblPlants; AET01770; AET01770; MTR_8g020840.
DR GeneID; 11434050; -.
DR Gramene; AET01770; AET01770; MTR_8g020840.
DR KEGG; mtr:MTR_8g020840; -.
DR eggNOG; ENOG502QSHA; Eukaryota.
DR HOGENOM; CLU_011924_5_1_1; -.
DR OMA; TEPRFFQ; -.
DR OrthoDB; 454341at2759; -.
DR Proteomes; UP000002051; Chromosome 8.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009610; P:response to symbiotic fungus; IEA:UniProt.
DR InterPro; IPR030015; SCL29/NSP1.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF243; PTHR31636:SF243; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Nodulation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..554
FT /note="Protein NODULATION SIGNALING PATHWAY 1"
FT /id="PRO_0000132232"
FT DOMAIN 159..548
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 76..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 166..227
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 246..315
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 331..373
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 383..468
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 471..548
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 281..285
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 76..93
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..111
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..165
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 240..554
FT /note="Missing: In nsp1-1; loss of nodulation."
FT /evidence="ECO:0000269|PubMed:15961669"
FT MUTAGEN 488..554
FT /note="Missing: In nsp1-2; loss of nodulation."
FT /evidence="ECO:0000269|PubMed:15961669"
SQ SEQUENCE 554 AA; 61827 MW; B87E941794563923 CRC64;
MTMEPNPTSD HILDWLEGSV SFFPSFLDDP YNNGYIHEYE IWNQNQDISN QYQIDANTNS
SNATNSTTNI VAASTTTSTT SLEPNSFNNI PFSDLPKKRN AEDELSLKKQ PQNQKNKRLK
SRPMNESDNG DAALEGTVVR KSGGNKKGAA KANGSNSNNG NNKDGRWAEQ LLNPCAVAIT
GGNLNRVQHL LYVLHELAST TGDANHRLAA HGLRALTHHL SSSSSSTPSG TITFASTEPR
FFQKSLLKFY EFSPWFSFPN NIANASILQV LAEEPNNLRT LHILDIGVSH GVQWPTFLEA
LSRRPGGPPP LVRLTVVNAS SSTENDQNME TPFSIGPCGD TFSSGLLGYA QSLNVNLQIK
KLDNHPLQTL NAKSVDTSSD ETLIVCAQFR LHHLNHNNPD ERSEFLKVLR GMEPKGVILS
ENNMECCCSS CGDFATGFSR RVEYLWRFLD STSSAFKNRD SDERKMMEGE AAKALTNQRE
MNERREKWCE RMKEAGFAGE VFGEDAIDGG RALLRKYDNN WEMKVEENST SVELWWKSQP
VSFCSLWKLD KQPE
