ID   NSP1_ROTHC              Reviewed;         394 AA.
AC   Q9PY94;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Non-structural protein 1 {ECO:0000255|HAMAP-Rule:MF_04088};
DE            Short=NSP1 {ECO:0000255|HAMAP-Rule:MF_04088};
DE   AltName: Full=NCVP2 {ECO:0000255|HAMAP-Rule:MF_04088};
DE   AltName: Full=Non-structural RNA-binding protein 53 {ECO:0000255|HAMAP-Rule:MF_04088};
DE            Short=NS53 {ECO:0000255|HAMAP-Rule:MF_04088};
OS   Rotavirus C (isolate RVC/Human/United Kingdom/Bristol/1989) (RV-C).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Rotavirus.
OX   NCBI_TaxID=31567;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10567650; DOI=10.1099/0022-1317-80-12-3181;
RA   James V.L., Lambden P.R., Deng Y., Caul E.O., Clarke I.N.;
RT   "Molecular characterization of human group C rotavirus genes 6, 7 and 9.";
RL   J. Gen. Virol. 80:3181-3187(1999).
CC   -!- FUNCTION: Plays a role in the inhibition of host innate immunity by
CC       inducing the degradation of key host factors required to activate
CC       interferon production such as IRF3, IRF5 or IRF7. Associates with
CC       components of cullin RING ligases (CRLs) including CUL1 or CUL3, which
CC       are essential multisubunit ubiquitination complexes, to modulate their
CC       activities. {ECO:0000255|HAMAP-Rule:MF_04088}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with host IRF3; this interaction
CC       leads to IRF3 degradation. Interacts with host IRF7; this interaction
CC       leads to IRF7 degradation. Interacts with host CUL1 and CUL3.
CC       {ECO:0000255|HAMAP-Rule:MF_04088}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm, host cytoskeleton
CC       {ECO:0000255|HAMAP-Rule:MF_04088}.
CC   -!- DOMAIN: The integrity of the zinc-binding domain in NSP1 is important
CC       for degradation of host IRF3. {ECO:0000255|HAMAP-Rule:MF_04088}.
CC   -!- SIMILARITY: Belongs to the rotavirus NSP1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_04088}.
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DR   EMBL; AJ132204; CAB52752.1; -; Genomic_RNA.
DR   RefSeq; YP_392487.1; NC_007544.1.
DR   GeneID; 3773134; -.
DR   KEGG; vg:3773134; -.
DR   Proteomes; UP000007664; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0039548; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0039557; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04088; ROTA_NSP1; 1.
DR   InterPro; IPR002148; Rotavirus_NSP1.
DR   Pfam; PF00981; Rota_NS53; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host cytoskeleton; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host IRF3 by virus; Inhibition of host IRF7 by virus;
KW   Inhibition of host RLR pathway by virus;
KW   Interferon antiviral system evasion; Metal-binding; Reference proteome;
KW   RNA-binding; Viral immunoevasion.
FT   CHAIN           1..394
FT                   /note="Non-structural protein 1"
FT                   /id="PRO_0000369881"
FT   REGION          2..80
FT                   /note="RNA-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04088"
FT   REGION          40..78
FT                   /note="Zinc-binding domain"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04088"
FT   REGION          81..171
FT                   /note="Important for cytoskeleton localization"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04088"
FT   REGION          268..394
FT                   /note="Interaction with host IRF3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04088"
SQ   SEQUENCE   394 AA;  46642 MW;  4FAB1BB841EDCF29 CRC64;
     MANSFREMLY WYRKIIDRKL PCVNVNIWRR EIAYKANGIC LNCLNECKVC PCDYCGIRHK
     CENCLNSDCF MNTNNEFNSH RWITFDEEPS QMVLFEYWIM YKDYFLSKFN YNYKAQLKIL
     NMNKNRRFHI NESKKKALSV PITSQYLKFK FNNKIYIMFG TFLTSKIQPW IQLKSLKVGY
     IQSLNVDRCA KLIATKGMFA TNSFKSSCIT EINARRPISE CDYLIEACLC NENNEWKFSA
     VMGRDKIPVT KSLAMKYFCK NINTELFYYG HSKCHVVSEC PRWNQQLRVL NASTLNIIFR
     RQFMNEVVEW FENFTQLTGM HYDFIKTCVY NKVIISHFRK EIEDYINSGK KISLSSVIPD
     GHALYTNIDI LRISLMLAID VALNRIESQQ MDVL