NSP2_LOTJA
ID NSP2_LOTJA Reviewed; 499 AA.
AC Q2PEG7; A1DS15;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 25-MAY-2022, entry version 51.
DE RecName: Full=Protein NODULATION SIGNALING PATHWAY 2 {ECO:0000303|PubMed:17071642};
DE Short=LsNSP2 {ECO:0000303|PubMed:17071642};
DE AltName: Full=Transcription initiator for nodulation {ECO:0000312|EMBL:BAE72690.1};
GN Name=NSP2 {ECO:0000303|PubMed:17071642};
GN Synonyms=TINOD {ECO:0000312|EMBL:BAE72690.1};
OS Lotus japonicus (Lotus corniculatus var. japonicus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX NCBI_TaxID=34305;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Gifu / B-129;
RX PubMed=17244637; DOI=10.1093/dnares/dsl017;
RA Murakami Y., Miwa H., Imaizumi-Anraku H., Kouchi H., Downie J.A.,
RA Kawaguchi M., Kawasaki S.;
RT "Positional cloning identifies Lotus japonicus NSP2, a putative
RT transcription factor of the GRAS family, required for NIN and ENOD40 gene
RT expression in nodule initiation.";
RL DNA Res. 13:255-265(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=cv. Gifu / B-129;
RX PubMed=17071642; DOI=10.1104/pp.106.089508;
RA Heckmann A.B., Lombardo F., Miwa H., Perry J.A., Bunnewell S., Parniske M.,
RA Wang T.L., Downie J.A.;
RT "Lotus japonicus nodulation requires two GRAS domain regulators, one of
RT which is functionally conserved in a non-legume.";
RL Plant Physiol. 142:1739-1750(2006).
RN [3]
RP FUNCTION.
RX PubMed=20719766; DOI=10.1093/pcp/pcq124;
RA Yokota K., Soyano T., Kouchi H., Hayashi M.;
RT "Function of GRAS proteins in root nodule symbiosis is retained in homologs
RT of a non-legume, rice.";
RL Plant Cell Physiol. 51:1436-1442(2010).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=23335614; DOI=10.1093/pcp/pct008;
RA Murakami Y., Yokoyama H., Fukui R., Kawaguchi M.;
RT "Down-regulation of NSP2 expression in developmentally young regions of
RT Lotus japonicus roots in response to rhizobial inoculation.";
RL Plant Cell Physiol. 54:518-527(2013).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23926062; DOI=10.1093/pcp/pct114;
RA Takeda N., Tsuzuki S., Suzaki T., Parniske M., Kawaguchi M.;
RT "CERBERUS and NSP1 of Lotus japonicus are common symbiosis genes that
RT modulate arbuscular mycorrhiza development.";
RL Plant Cell Physiol. 54:1711-1723(2013).
RN [6]
RP INTERACTION WITH IPN2, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLU-224.
RX PubMed=24400899; DOI=10.1111/nph.12593;
RA Kang H., Chu X., Wang C., Xiao A., Zhu H., Yuan S., Yang Z., Ke D.,
RA Xiao S., Hong Z., Zhang Z.;
RT "A MYB coiled-coil transcription factor interacts with NSP2 and is involved
RT in nodulation in Lotus japonicus.";
RL New Phytol. 201:837-849(2014).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24329948; DOI=10.1111/tpj.12374;
RA Hayashi T., Shimoda Y., Sato S., Tabata S., Imaizumi-Anraku H., Hayashi M.;
RT "Rhizobial infection does not require cortical expression of upstream
RT common symbiosis genes responsible for the induction of Ca(2+) spiking.";
RL Plant J. 77:146-159(2014).
RN [8]
RP INTERACTION WITH RAD1, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Gifu / B-129;
RX PubMed=25560877; DOI=10.1104/pp.114.255430;
RA Xue L., Cui H., Buer B., Vijayakumar V., Delaux P.-M., Junkermann S.,
RA Bucher M.;
RT "Network of GRAS transcription factors involved in the control of arbuscule
RT development in Lotus japonicus.";
RL Plant Physiol. 167:854-871(2015).
CC -!- FUNCTION: Transcriptional regulator essential for Nod-factor-induced
CC gene expression (Probable) (PubMed:17244637, PubMed:17071642). Acts
CC downstream of calcium spiking and a calcium/calmodulin-dependent
CC protein kinase required for activation of early nodulation gene
CC expression (PubMed:17244637, PubMed:17071642, PubMed:24329948).
CC Transcription factor involved in the induction of NIN and ENOD40 genes,
CC which are required for rhizobial infection and early nodule development
CC (PubMed:17244637). Does not seem to contribute to the early steps of
CC the arbuscular mycorrhizal fungus infection and colonization processes
CC in roots (PubMed:23926062). Transcription factor involved in the
CC positive regulation of the beta-carotene isomerase D27, which
CC participates in a pathway leading to biosynthesis of strigolactones in
CC roots (PubMed:23926062). {ECO:0000269|PubMed:17071642,
CC ECO:0000269|PubMed:17244637, ECO:0000269|PubMed:23926062,
CC ECO:0000269|PubMed:24329948, ECO:0000305|PubMed:20719766,
CC ECO:0000305|PubMed:23335614}.
CC -!- SUBUNIT: Interacts with IPN2 (PubMed:24400899). Binds to RAD1
CC (PubMed:25560877). Interacts with RAM1 (By similarity).
CC {ECO:0000250|UniProtKB:Q5NE24, ECO:0000269|PubMed:24400899,
CC ECO:0000269|PubMed:25560877}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:17244637,
CC ECO:0000269|PubMed:24400899, ECO:0000269|PubMed:25560877}. Endoplasmic
CC reticulum {ECO:0000250|UniProtKB:Q5NE24}. Note=Mainly localized to the
CC nuclear envelope. Also found in the endoplasmic reticulum. Upon Nod-
CC factor application, the nuclear envelope localization disappears and
CC the protein accumulates in the nucleus. {ECO:0000250|UniProtKB:Q5NE24}.
CC -!- TISSUE SPECIFICITY: Highly expressed in roots.
CC {ECO:0000269|PubMed:17071642, ECO:0000269|PubMed:17244637}.
CC -!- INDUCTION: Induced in roots after inoculation with Mesorhizobium loti
CC (PubMed:17071642). Down-regulated in developmentally young regions of
CC roots after inoculation with Mesorhizobium loti (PubMed:23335614).
CC {ECO:0000269|PubMed:17071642, ECO:0000269|PubMed:23335614}.
CC -!- DISRUPTION PHENOTYPE: Roots of knockout plants form very few aborted
CC and non-functional nodules under nitrogen limitation when inoculated
CC with Mesorhizobium loti. {ECO:0000269|PubMed:17244637,
CC ECO:0000269|PubMed:23926062, ECO:0000269|PubMed:24329948}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000305}.
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DR EMBL; AB241456; BAE72690.1; -; Genomic_DNA.
DR EMBL; AB241457; BAE72691.1; -; mRNA.
DR EMBL; DQ665943; ABG49438.1; -; Genomic_DNA.
DR EMBL; EF053276; ABK35068.1; -; Genomic_DNA.
DR AlphaFoldDB; Q2PEG7; -.
DR SMR; Q2PEG7; -.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR InterPro; IPR030028; SCL26/NSP2.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF25; PTHR31636:SF25; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Nodulation; Nucleus; Transcription;
KW Transcription regulation.
FT CHAIN 1..499
FT /note="Protein NODULATION SIGNALING PATHWAY 2"
FT /id="PRO_0000448273"
FT DOMAIN 106..491
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 64..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..182
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 201..265
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 281..313
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 323..414
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 417..491
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 232..236
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 89..105
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 224
FT /note="E->K: Abolishes the interaction with IPN2; abolishes
FT transactivation activity."
FT /evidence="ECO:0000269|PubMed:24400899"
SQ SEQUENCE 499 AA; 54774 MW; 6D57AE4C0ACCDF45 CRC64;
MEMDIDCIHH LDFSGHSTLT NTPSSDNDNY GCSWNHWSPV VNWDAFTGNQ DDFHHLIDSM
IDDNNTGPAF SDHTASTTSE EEEEEEATTT TMTTTTTTTT TTPEAADDDF KGLRLVHLLM
AGAEALTGAN KNRELARVIL VRLKELVSHT DGTNMERLAA YFTEALQGLL EGAGGAYNSS
SKHHVIGGPH HEPQNDALAA FQLLQDMSPY VKFGHFTANQ AIVEAVAHER RVHIVDYDIM
EGVQWASLMQ ALASNPNGPH LRITALSRSG VGRRSMATVQ ETGRRLTAFA TSLGQPFSFH
HSRLESDETF RPAGLKLVRG EALVFNCMLN LPHLTYRSPN SVASFLTAAK ALRPRLVTVV
EEEVGSALGG FVERFMDSLH HFSAVFDSLE AGFPMQGRAR ALVERVFLGP RIVGSLARIY
RTGGGGEERG SWREWLRAAG FSGVAVSSAN HCQSNLLLGL FNDGYRVEEL GSNKLVLHWK
TRRLLSASLW TCSSESDCA
