NSP2_ORYSJ
ID NSP2_ORYSJ Reviewed; 575 AA.
AC Q84Q92;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protein NODULATION SIGNALING PATHWAY 2 {ECO:0000303|PubMed:20719766};
DE Short=OsNSP2 {ECO:0000303|PubMed:20719766};
DE AltName: Full=GRAS family protein 13 {ECO:0000303|PubMed:15316287};
DE Short=OsGRAS-13 {ECO:0000303|PubMed:15316287};
GN Name=NSP2 {ECO:0000303|PubMed:20719766};
GN OrderedLocusNames=Os03g0263300 {ECO:0000312|EMBL:BAS83378.1},
GN LOC_Os03g15680 {ECO:0000312|EMBL:ABF95108.1};
GN ORFNames=OJ1041F02.13 {ECO:0000312|EMBL:AAP06856.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP GENE FAMILY.
RX PubMed=15316287; DOI=10.1023/b:plan.0000038256.89809.57;
RA Tian C., Wan P., Sun S., Li J., Chen M.;
RT "Genome-wide analysis of the GRAS gene family in rice and Arabidopsis.";
RL Plant Mol. Biol. 54:519-532(2004).
RN [6]
RP FUNCTION.
RX PubMed=20719766; DOI=10.1093/pcp/pcq124;
RA Yokota K., Soyano T., Kouchi H., Hayashi M.;
RT "Function of GRAS proteins in root nodule symbiosis is retained in homologs
RT of a non-legume, rice.";
RL Plant Cell Physiol. 51:1436-1442(2010).
RN [7]
RP FUNCTION.
RX PubMed=22039214; DOI=10.1105/tpc.111.089771;
RA Liu W., Kohlen W., Lillo A., Op den Camp R., Ivanov S., Hartog M.,
RA Limpens E., Jamil M., Smaczniak C., Kaufmann K., Yang W.C., Hooiveld G.J.,
RA Charnikhova T., Bouwmeester H.J., Bisseling T., Geurts R.;
RT "Strigolactone biosynthesis in Medicago truncatula and rice requires the
RT symbiotic GRAS-type transcription factors NSP1 and NSP2.";
RL Plant Cell 23:3853-3865(2011).
RN [8]
RP INTERACTION WITH SMOS1.
RX PubMed=28069545; DOI=10.1016/j.molp.2016.12.013;
RA Hirano K., Yoshida H., Aya K., Kawamura M., Hayashi M., Hobo T.,
RA Sato-Izawa K., Kitano H., Ueguchi-Tanaka M., Matsuoka M.;
RT "SMALL ORGAN SIZE 1 and SMALL ORGAN SIZE 2/DWARF AND LOW-TILLERING form a
RT complex to integrate auxin and brassinosteroid signaling in rice.";
RL Mol. Plant 10:590-604(2017).
CC -!- FUNCTION: Transcription factor involved in the control of strigolactone
CC biosynthesis in roots through the activation of the beta-carotene
CC isomerase D27, which participates in a pathway leading to biosynthesis
CC of strigolactones. {ECO:0000269|PubMed:22039214}.
CC -!- SUBUNIT: Interacts with SMOS1. {ECO:0000269|PubMed:28069545}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots.
CC {ECO:0000269|PubMed:22039214}.
CC -!- MISCELLANEOUS: Plants silencing NSP1 and NSP2 exhibit reduced
CC expression of the strigolactone biosynthetic gene D27, reduced levels
CC of strigolactones and increased shoot tillering (PubMed:22039214). Rice
CC NSP2 is a homolog of Lotus japonicus and Medicago truncatula NSP2
CC genes, which are transcriptional regulators essential for Nod-factor-
CC induced gene expression (Probable). Although rice roots cannot form
CC root nodule symbiosis, rice NSP2 is able to complement a NSP2-deficient
CC mutant in Lotus japonicus (PubMed:20719766). Rice NSP2 is able to
CC complement a NSP2-deficient mutant in Medicago truncatula
CC (PubMed:22039214). {ECO:0000269|PubMed:20719766,
CC ECO:0000269|PubMed:22039214, ECO:0000305|PubMed:20719766,
CC ECO:0000305|PubMed:22039214}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC135206; AAP06856.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF95108.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83378.1; -; Genomic_DNA.
DR RefSeq; XP_015630025.1; XM_015774539.1.
DR AlphaFoldDB; Q84Q92; -.
DR SMR; Q84Q92; -.
DR STRING; 39947.Q84Q92; -.
DR PaxDb; Q84Q92; -.
DR PRIDE; Q84Q92; -.
DR EnsemblPlants; Os03t0263300-01; Os03t0263300-01; Os03g0263300.
DR GeneID; 107276070; -.
DR Gramene; Os03t0263300-01; Os03t0263300-01; Os03g0263300.
DR KEGG; osa:107276070; -.
DR InParanoid; Q84Q92; -.
DR OrthoDB; 542322at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR030028; SCL26/NSP2.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF25; PTHR31636:SF25; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..575
FT /note="Protein NODULATION SIGNALING PATHWAY 2"
FT /id="PRO_0000448271"
FT DOMAIN 123..526
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 103..123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 130..202
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 221..286
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 302..334
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 344..450
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 453..526
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 252..256
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 103..118
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 575 AA; 60848 MW; 65BC2643B3936684 CRC64;
MEVTMEDVAG DFEFSGCGST TTTSSASSLD DGTGMCYAWG ELSPVADWAN FCCSDDDGGH
DLHGLIESML CDDTLVGVDD DGQAGLHHAD MFRDDLYCYG NGSNPSSTTT TNPGSPVFDD
PTQGCPEKGL RLLHLLMAAA EALSGPHKSR ELARVILVRL KEMVSHTASA NAAASNMERL
AAHFTDALQG LLDGSHPVGG SGRQAAAAAS HHHAGDVLTA FQMLQDMSPY MKFGHFTANQ
AILEAVSGDR RVHIVDYDIA EGIQWASLMQ AMTSRADGVP APHLRITAVS RSGGGGARAV
QEAGRRLSAF AASIGQPFSF GQCRLDSDER FRPATVRMVK GEALVANCVL HQAAATTTIR
RPTGSVASFL SGMAALGAKL VTVVEEEGEA EKDDDGDSAG DAAAGGFVRQ FMEELHRYSA
VWDSLEAGFP TQSRVRGLVE RVILAPNIAG AVSRAYRGVD GEGRCGWGQW MRGSGFTAVP
LSCFNHSQAR LLLGLFNDGY TVEETGPNKI VLGWKARRLM SASVWAPPPL PVPSSPPEGV
CQPVVGMAPV ATGGFARTEF DYIDSFLVEP AYALV
