ID   NSP3_ROTH6              Reviewed;         313 AA.
AC   Q82051; B3SRQ5;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   29-SEP-2021, entry version 65.
DE   RecName: Full=Non-structural protein 3 {ECO:0000255|HAMAP-Rule:MF_04094};
DE            Short=NSP3 {ECO:0000255|HAMAP-Rule:MF_04094};
DE   AltName: Full=NCVP4 {ECO:0000255|HAMAP-Rule:MF_04094};
DE   AltName: Full=Non-structural RNA-binding protein 34 {ECO:0000255|HAMAP-Rule:MF_04094};
DE            Short=NS34 {ECO:0000255|HAMAP-Rule:MF_04094};
OS   Rotavirus A (strain RVA/Human/Indonesia/69M/1980/G8P4[10]) (RV-A).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A.
OX   NCBI_TaxID=10947;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7871749; DOI=10.1006/viro.1995.1087;
RA   Rao C.D., Das M., Ilango P., Lalwani R., Rao B.S., Gowda K.;
RT   "Comparative nucleotide and amino acid sequence analysis of the sequence-
RT   specific RNA-binding rotavirus nonstructural protein NSP3.";
RL   Virology 207:327-333(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-313.
RX   PubMed=18786998; DOI=10.1128/jvi.01402-08;
RA   Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T.,
RA   Patton J.T.;
RT   "Group A human rotavirus genomics: evidence that gene constellations are
RT   influenced by viral protein interactions.";
RL   J. Virol. 82:11106-11116(2008).
CC   -!- FUNCTION: Plays an important role in stimulating the translation of
CC       viral mRNAs. These mRNAs are capped but not polyadenylated, instead
CC       terminating in a conserved sequence 'GACC' at the 3' that is recognized
CC       by NSP3, which competes with host PABPC1 for EIF4G1 binding. The
CC       interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1
CC       interaction, thereby facilitating the initiation of capped mRNA
CC       translation. {ECO:0000255|HAMAP-Rule:MF_04094}.
CC   -!- SUBUNIT: Homodimer. Interacts (via the coiled-coil region) with host
CC       ZC3H7B (via LD motif). Interacts with host EIF4G1. {ECO:0000255|HAMAP-
CC       Rule:MF_04094}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04094}.
CC   -!- SIMILARITY: Belongs to the rotavirus NSP3 family. {ECO:0000255|HAMAP-
CC       Rule:MF_04094}.
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DR   EMBL; X81425; CAA57184.1; -; mRNA.
DR   EMBL; EF672558; ABV53230.1; -; Genomic_DNA.
DR   PIR; S51728; S51728.
DR   SMR; Q82051; -.
DR   Proteomes; UP000001455; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 6.10.280.20; -; 1.
DR   HAMAP; MF_04094; ROTA_A_NSP3; 1.
DR   HAMAP; MF_04090; ROTA_NSP3; 1.
DR   InterPro; IPR042519; NSP3_N_rotavirus.
DR   InterPro; IPR036082; NSP3_sf.
DR   InterPro; IPR002873; Rotavirus_NSP3.
DR   Pfam; PF01665; Rota_NSP3; 1.
DR   SUPFAM; SSF69903; SSF69903; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Host cytoplasm; Host-virus interaction; RNA-binding;
KW   Translation regulation.
FT   CHAIN           1..313
FT                   /note="Non-structural protein 3"
FT                   /id="PRO_0000369448"
FT   REGION          1..149
FT                   /note="RNA-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          150..206
FT                   /note="Dimerization"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          170..234
FT                   /note="Interaction with host ZC3H7B"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          208..313
FT                   /note="Interaction with host EIF4G1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   COILED          166..237
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   CONFLICT        45
FT                   /note="R -> K (in Ref. 2; ABV53230)"
FT   CONFLICT        94
FT                   /note="D -> V (in Ref. 2; ABV53230)"
FT   CONFLICT        202
FT                   /note="D -> V (in Ref. 2; ABV53230)"
FT   CONFLICT        207
FT                   /note="Y -> N (in Ref. 2; ABV53230)"
FT   CONFLICT        278
FT                   /note="Y -> F (in Ref. 2; ABV53230)"
SQ   SEQUENCE   313 AA;  36493 MW;  98022A6F7EE5514D CRC64;
     MLKMESTQQM VSSIINSSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF DLVMDDSGVK
     NNLMGKAATI DQALNGKFSS SIRNRNWMTD SKTDARLDED VNKLRLLLSS KGIDQKMRVL
     NACFNVKRIP GKSSSVIRCT RLMKEKIERG EVEVDDAFVE EKMEVDTIDW KSRYEQLEKR
     FESLKQRVNE KYNNWVIKAR KDNENMYSLQ NVISQQQAHI NELQIYNNKL ERDLQSKIGS
     VISSIEWYLR SMELSDDIKS DIEQQLNSID QINPVNAYDD FESILRNLIS DYDRMFIMFK
     GLLQQSNYIY TYE