ID   NSP3_ROTHL              Reviewed;         313 AA.
AC   A3DSK9; B3SRU5;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-APR-2009, sequence version 2.
DT   29-SEP-2021, entry version 39.
DE   RecName: Full=Non-structural protein 3 {ECO:0000255|HAMAP-Rule:MF_04094};
DE            Short=NSP3 {ECO:0000255|HAMAP-Rule:MF_04094};
DE   AltName: Full=NCVP4 {ECO:0000255|HAMAP-Rule:MF_04094};
DE   AltName: Full=Non-structural RNA-binding protein 34 {ECO:0000255|HAMAP-Rule:MF_04094};
DE            Short=NS34 {ECO:0000255|HAMAP-Rule:MF_04094};
OS   Rotavirus A (strain RVA/Human/Philippines/L26/1987/G12P1B[4]) (RV-A).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A.
OX   NCBI_TaxID=10953;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=17166908; DOI=10.1128/jvi.01622-06;
RA   Rahman M., Matthijnssens J., Yang X., Delbeke T., Arijs I., Taniguchi K.,
RA   Iturriza-Gomara M., Iftekharuddin N., Azim T., Van Ranst M.;
RT   "Evolutionary history and global spread of the emerging G12 human
RT   rotaviruses.";
RL   J. Virol. 81:2382-2390(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=18786998; DOI=10.1128/jvi.01402-08;
RA   Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T.,
RA   Patton J.T.;
RT   "Group A human rotavirus genomics: evidence that gene constellations are
RT   influenced by viral protein interactions.";
RL   J. Virol. 82:11106-11116(2008).
CC   -!- FUNCTION: Plays an important role in stimulating the translation of
CC       viral mRNAs. These mRNAs are capped but not polyadenylated, instead
CC       terminating in a conserved sequence 'GACC' at the 3' that is recognized
CC       by NSP3, which competes with host PABPC1 for EIF4G1 binding. The
CC       interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1
CC       interaction, thereby facilitating the initiation of capped mRNA
CC       translation. {ECO:0000255|HAMAP-Rule:MF_04094}.
CC   -!- SUBUNIT: Homodimer. Interacts (via the coiled-coil region) with host
CC       ZC3H7B (via LD motif). Interacts with host EIF4G1. {ECO:0000255|HAMAP-
CC       Rule:MF_04094}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04094}.
CC   -!- SIMILARITY: Belongs to the rotavirus NSP3 family. {ECO:0000255|HAMAP-
CC       Rule:MF_04094}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABA34239.1; Type=Erroneous initiation;
CC       Sequence=ABV53270.1; Type=Erroneous initiation;
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DR   EMBL; DQ146697; ABA34239.1; ALT_INIT; Genomic_RNA.
DR   EMBL; EF672593; ABV53270.1; ALT_INIT; Genomic_RNA.
DR   SMR; A3DSK9; -.
DR   Proteomes; UP000001459; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 6.10.280.20; -; 1.
DR   HAMAP; MF_04094; ROTA_A_NSP3; 1.
DR   HAMAP; MF_04090; ROTA_NSP3; 1.
DR   InterPro; IPR042519; NSP3_N_rotavirus.
DR   InterPro; IPR036082; NSP3_sf.
DR   InterPro; IPR002873; Rotavirus_NSP3.
DR   Pfam; PF01665; Rota_NSP3; 1.
DR   SUPFAM; SSF69903; SSF69903; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Host cytoplasm; Host-virus interaction; RNA-binding;
KW   Translation regulation.
FT   CHAIN           1..313
FT                   /note="Non-structural protein 3"
FT                   /id="PRO_0000369452"
FT   REGION          1..149
FT                   /note="RNA-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          150..206
FT                   /note="Dimerization"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          170..234
FT                   /note="Interaction with host ZC3H7B"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   REGION          208..313
FT                   /note="Interaction with host EIF4G1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT   COILED          166..237
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
SQ   SEQUENCE   313 AA;  36411 MW;  2EDB0F1DC55C5130 CRC64;
     MLKMESTQQM ASSIINSSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF DFVMDDSGVK
     NNLMGKAATI DQALNGKFSS SIRNRNWMTD SKTVARLDED VNKLRLLLSS KGIDQKMRVL
     NACFNVKRVP GKSSSVIKCT RLMKDKIERG EVEVDDTFVE ERMEIDTIDW KSRYDQLERR
     FESLKQRVNE KYNNWVIKAR KVNENMNSLQ NVISQQQAHI NELQIYNNKL ERDLQSKIGS
     VISSIEWYLR SMELSDDIKS DIEQQLNSID HINPVNAFDD FESILRNLIS DYDRIFIMFK
     GLLQQSNYTY TYE