NSP3_ROTRF
ID NSP3_ROTRF Reviewed; 313 AA.
AC Q86504;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 29-SEP-2021, entry version 68.
DE RecName: Full=Non-structural protein 3 {ECO:0000255|HAMAP-Rule:MF_04094};
DE Short=NSP3 {ECO:0000255|HAMAP-Rule:MF_04094};
DE AltName: Full=NCVP4 {ECO:0000255|HAMAP-Rule:MF_04094};
DE AltName: Full=Non-structural RNA-binding protein 34 {ECO:0000255|HAMAP-Rule:MF_04094};
DE Short=NS34 {ECO:0000255|HAMAP-Rule:MF_04094};
OS Rotavirus A (strain RVA/Cow/France/RF/1975/G6P6[1]) (RV-A).
OC Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A.
OX NCBI_TaxID=10933;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8240020; DOI=10.1007/bf01309746;
RA Aponte C., Mattion N.M., Estes M.K., Charpilienne A., Cohen J.;
RT "Expression of two bovine rotavirus non-structural proteins (NSP2, NSP3) in
RT the baculovirus system and production of monoclonal antibodies directed
RT against the expressed proteins.";
RL Arch. Virol. 133:85-95(1993).
RN [2]
RP INTERACTION WITH HUMAN ZC3H7B.
RX PubMed=15047801; DOI=10.1128/jvi.78.8.3851-3862.2004;
RA Vitour D., Lindenbaum P., Vende P., Becker M.M., Poncet D.;
RT "RoXaN, a novel cellular protein containing TPR, LD, and zinc finger
RT motifs, forms a ternary complex with eukaryotic initiation factor 4G and
RT rotavirus NSP3.";
RL J. Virol. 78:3851-3862(2004).
RN [3]
RP INTERACTION WITH HUMAN ZC3H7B, AND INTERACTION WITH HUMAN EIF4G1.
RX PubMed=18799579; DOI=10.1128/jvi.00872-08;
RA Harb M., Becker M.M., Vitour D., Baron C.H., Vende P., Brown S.C.,
RA Bolte S., Arold S.T., Poncet D.;
RT "Nuclear localization of cytoplasmic poly(A)-binding protein upon rotavirus
RT infection involves the interaction of NSP3 with eIF4G and RoXaN.";
RL J. Virol. 82:11283-11293(2008).
CC -!- FUNCTION: Plays an important role in stimulating the translation of
CC viral mRNAs. These mRNAs are capped but not polyadenylated, instead
CC terminating in a conserved sequence 'GACC' at the 3' that is recognized
CC by NSP3, which competes with host PABPC1 for EIF4G1 binding. The
CC interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1
CC interaction, thereby facilitating the initiation of capped mRNA
CC translation. {ECO:0000255|HAMAP-Rule:MF_04094}.
CC -!- SUBUNIT: Homodimer. Interacts (via the coiled-coil region) with host
CC ZC3H7B (via LD motif). Interacts with host EIF4G1. {ECO:0000255|HAMAP-
CC Rule:MF_04094, ECO:0000269|PubMed:15047801,
CC ECO:0000269|PubMed:18799579}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04094}.
CC -!- SIMILARITY: Belongs to the rotavirus NSP3 family. {ECO:0000255|HAMAP-
CC Rule:MF_04094}.
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DR EMBL; Z21639; CAA79754.1; -; mRNA.
DR PIR; S49006; S49006.
DR SMR; Q86504; -.
DR DIP; DIP-1160N; -.
DR Proteomes; UP000007179; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR Gene3D; 6.10.280.20; -; 1.
DR HAMAP; MF_04094; ROTA_A_NSP3; 1.
DR HAMAP; MF_04090; ROTA_NSP3; 1.
DR InterPro; IPR042519; NSP3_N_rotavirus.
DR InterPro; IPR036082; NSP3_sf.
DR InterPro; IPR002873; Rotavirus_NSP3.
DR Pfam; PF01665; Rota_NSP3; 1.
DR SUPFAM; SSF69903; SSF69903; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Host cytoplasm; Host-virus interaction; RNA-binding;
KW Translation regulation.
FT CHAIN 1..313
FT /note="Non-structural protein 3"
FT /id="PRO_0000367816"
FT REGION 1..149
FT /note="RNA-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT REGION 150..206
FT /note="Dimerization"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT REGION 170..234
FT /note="Interaction with host ZC3H7B"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT REGION 170..234
FT /note="Interaction with ZC3H7B"
FT REGION 208..313
FT /note="Interaction with host EIF4G1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
FT COILED 166..237
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04094"
SQ SEQUENCE 313 AA; 36347 MW; 2AD38815AAEB349B CRC64;
MLKMESTQQM ASSIINTSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF DYVMDDSGVK
NNLLGKAATI DQALNGKFGS AARNRNWMAD TRTTARLDED VNKLRMMLSS KGIDQKMRVL
NACFNVKRVP GKSSSIIKCT RLMRDKIERG EVEVDDSFVE EKMEVDTIDW KSRYEQLEKR
FESLKQRVNE KYTSWVQKAK KVNENMYSLQ NVISQQQSQI ADLQNYCNKL EVDLQNKISS
LVSSVEWYLK SMELPDEIKT DIEQQLNSID VINPINAIDD FESLIRNIIL DYDRIFLMFK
GLMRQCNYEY TYE
