NSP5_ARATH
ID NSP5_ARATH Reviewed; 326 AA.
AC Q93XW5; Q8LDQ6; Q9LUB5;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Nitrile-specifier protein 5;
DE Short=AtNSP5;
GN Name=NSP5; OrderedLocusNames=At5g48180; ORFNames=MIF21.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18987211; DOI=10.1104/pp.108.130732;
RA Burow M., Losansky A., Muller R., Plock A., Kliebenstein D.J.,
RA Wittstock U.;
RT "The genetic basis of constitutive and herbivore-induced ESP-independent
RT nitrile formation in Arabidopsis.";
RL Plant Physiol. 149:561-574(2009).
CC -!- FUNCTION: Promotes simple nitriles, but not epithionitrile or
CC thiocyanate formation. Converts allylglucosinolate and
CC benzylglucosinolate to their corresponding simple nitriles in the
CC presence of myrosinase. {ECO:0000269|PubMed:18987211}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:18987211}.
CC -!- MISCELLANEOUS: Does not contain the mannose-binding lectin domain
CC present in other members of the family.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA96998.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB023039; BAA96998.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95630.1; -; Genomic_DNA.
DR EMBL; AY059952; AAL24434.1; -; mRNA.
DR EMBL; AY081619; AAM10181.1; -; mRNA.
DR EMBL; AY085861; AAM63074.1; -; mRNA.
DR RefSeq; NP_568692.1; NM_124193.4.
DR AlphaFoldDB; Q93XW5; -.
DR SMR; Q93XW5; -.
DR BioGRID; 20118; 1.
DR STRING; 3702.AT5G48180.1; -.
DR iPTMnet; Q93XW5; -.
DR SwissPalm; Q93XW5; -.
DR PaxDb; Q93XW5; -.
DR PRIDE; Q93XW5; -.
DR ProteomicsDB; 250495; -.
DR DNASU; 834871; -.
DR EnsemblPlants; AT5G48180.1; AT5G48180.1; AT5G48180.
DR GeneID; 834871; -.
DR Gramene; AT5G48180.1; AT5G48180.1; AT5G48180.
DR KEGG; ath:AT5G48180; -.
DR Araport; AT5G48180; -.
DR TAIR; locus:2164733; AT5G48180.
DR eggNOG; KOG0379; Eukaryota.
DR HOGENOM; CLU_030461_0_0_1; -.
DR InParanoid; Q93XW5; -.
DR OMA; QWKAVET; -.
DR OrthoDB; 933937at2759; -.
DR PhylomeDB; Q93XW5; -.
DR PRO; PR:Q93XW5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q93XW5; baseline and differential.
DR Genevisible; Q93XW5; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR GO; GO:0019762; P:glucosinolate catabolic process; IDA:TAIR.
DR GO; GO:0080028; P:nitrile biosynthetic process; IDA:TAIR.
DR Gene3D; 2.120.10.80; -; 2.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR Pfam; PF01344; Kelch_1; 3.
DR SUPFAM; SSF117281; SSF117281; 1.
PE 2: Evidence at transcript level;
KW Kelch repeat; Reference proteome; Repeat.
FT CHAIN 1..326
FT /note="Nitrile-specifier protein 5"
FT /id="PRO_0000363146"
FT REPEAT 32..83
FT /note="Kelch 1"
FT REPEAT 84..130
FT /note="Kelch 2"
FT REPEAT 134..183
FT /note="Kelch 3"
FT REPEAT 185..232
FT /note="Kelch 4"
FT CONFLICT 56
FT /note="E -> G (in Ref. 4; AAM63074)"
FT /evidence="ECO:0000305"
FT CONFLICT 114
FT /note="A -> S (in Ref. 4; AAM63074)"
FT /evidence="ECO:0000305"
FT CONFLICT 139
FT /note="G -> R (in Ref. 4; AAM63074)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 326 AA; 35853 MW; 42F5CF2D23963A19 CRC64;
MCPVENKWLK VGQKGAGPGA RSSHAMTVVG NKVYCFGGEL KPTIHIDNDL YVFDLETQEW
SIAPATGEAP FPCFGVSMVT IGSTIYVYGG RDDKRRYNGL HSYDTETNEW KLLAPVEEGL
PGRSYHSMAG DDRKVYVFGG VTAKGRVNTL HAYDVVDQKW VEYPAAGEAC KGRGAPGLVV
VEGRIWVLFG FDGNELGDIH CFDLASEQWK AVETTGDVPA ARSVFPAVSY GKYIVIYGGE
EEPHELMHMG AGKMSGEVYQ LDTETLVWER IVCGNEEEKP SQRGWCAFTK AVKDGEEGLL
VHGGNSPTNE RLDDLVFWGF SHLNVN
