NSP_ABMVW
ID NSP_ABMVW Reviewed; 256 AA.
AC P21945;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 3.
DT 02-JUN-2021, entry version 94.
DE RecName: Full=Nuclear shuttle protein;
DE Short=NSP;
DE AltName: Full=Protein BR1;
DE AltName: Full=Protein BV1;
GN ORFNames=BR1, BV1;
OS Abutilon mosaic virus (isolate West India) (AbMV).
OC Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC Geplafuvirales; Geminiviridae; Begomovirus.
OX NCBI_TaxID=10816;
OH NCBI_TaxID=3630; Abutilon.
OH NCBI_TaxID=3635; Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OH NCBI_TaxID=47605; Hibiscus.
OH NCBI_TaxID=96479; Malva.
OH NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH NCBI_TaxID=108335; Sida.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2219703; DOI=10.1016/0042-6822(90)90343-p;
RA Frischmuth T., Zimmat G., Jeske H.;
RT "The nucleotide sequence of abutilon mosaic virus reveals prokaryotic as
RT well as eukaryotic features.";
RL Virology 178:461-468(1990).
RN [2]
RP SEQUENCE REVISION TO 112; 232 AND C-TERMINUS.
RA Jeske H.;
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=11883189; DOI=10.1006/viro.2001.1185;
RA Zhang S.C., Wege C., Jeske H.;
RT "Movement proteins (BC1 and BV1) of Abutilon mosaic geminivirus are
RT cotransported in and between cells of sink but not of source leaves as
RT detected by green fluorescent protein tagging.";
RL Virology 290:249-260(2001).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=15220444; DOI=10.1128/jvi.78.14.7698-7706.2004;
RA Hehnle S., Wege C., Jeske H.;
RT "Interaction of DNA with the movement proteins of geminiviruses
RT revisited.";
RL J. Virol. 78:7698-7706(2004).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=17351736; DOI=10.1007/s00709-006-0223-x;
RA Frischmuth S., Wege C., Huelsefr D., Jeske H.;
RT "The movement protein BC1 promotes redirection of the nuclear shuttle
RT protein BV1 of Abutilon mosaic geminivirus to the plasma membrane in
RT fission yeast.";
RL Protoplasma 230:117-123(2007).
CC -!- FUNCTION: Binds to the genomic viral ssDNA, shuttles it into and out of
CC the cell nucleus. Begomoviruses use 2 proteins to transport their DNA
CC from cell to cell. The nuclear shuttle protein (NSP) shuttles it
CC between nucleus and cytoplasm and the movement protein (MP) probably
CC transports the DNA-NSP complex to the cell periphery and facilitates
CC movement across the cell wall. {ECO:0000269|PubMed:15220444}.
CC -!- SUBUNIT: Binds to single-stranded and double-stranded viral DNA.
CC Interacts with the host nuclear shuttle interacting (NSI) protein. This
CC interaction may allow NSP to recruit NSI monomers to the viral genome
CC and thus regulate nuclear export of viral genome by NSP (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host nucleus. Host cytoplasm. Host cell membrane;
CC Peripheral membrane protein; Cytoplasmic side. Note=Translocated to the
CC plasma membrane by the movement protein BC1.
CC -!- SIMILARITY: Belongs to the begomovirus nuclear shuttle protein family.
CC {ECO:0000305}.
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DR EMBL; X15984; CAA34114.3; -; Genomic_DNA.
DR PIR; E36214; QQCVW5.
DR RefSeq; NP_047219.2; NC_001929.2.
DR GeneID; 956374; -.
DR KEGG; vg:956374; -.
DR Proteomes; UP000006885; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0051027; P:DNA transport; IEA:InterPro.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR001530; Gemini_BR1.
DR InterPro; IPR000263; GV_A/BR1_coat.
DR Pfam; PF00844; Gemini_coat; 1.
DR PRINTS; PR00223; GEMCOATARBR1.
DR PRINTS; PR00225; GEMCOATBR1.
PE 1: Evidence at protein level;
KW DNA-binding; Host cell membrane; Host cytoplasm; Host membrane;
KW Host nucleus; Host-virus interaction; Membrane; Transport;
KW Viral movement protein.
FT CHAIN 1..256
FT /note="Nuclear shuttle protein"
FT /id="PRO_0000222260"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..187
FT /note="Interaction with Arabidopsis thaliana NSI protein"
FT /evidence="ECO:0000250"
FT MOTIF 21..42
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000250"
FT MOTIF 81..96
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 19..33
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 256 AA; 29514 MW; 4FC88622EF89D43A CRC64;
MYPSRNKRGS YFNQRRQYSR NHVWKRPTAA KRHDWKRRPS NTSKPNDEPK MSAQRIHENQ
YGPEFVMAQN SAISSFISYP DLGRSEPNRS RSYIRLKQLR FKGTVKIEQV PLAMNMDGST
PKVEGVFSLV IVVDRKPHLG PSGCLHTFDE LFGARIHSHG NLSVTPALKD RYYIRHVCKR
VLSVEKDTLM VDVEGSIPLS NRRINCWATF KDVDRESCKG VYDNISKNAL LVYYCWMSDT
PAKASTFVSF DLDYIG
