ID   NSP_CLVN                Reviewed;         256 AA.
AC   P14970;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Nuclear shuttle protein;
DE            Short=NSP;
DE   AltName: Full=29.4 kDa protein;
DE   AltName: Full=Protein BR1;
DE   AltName: Full=Protein BV1;
GN   ORFNames=BR1, BV1;
OS   African cassava mosaic virus (isolate Nigerian) (ACMV) (Cassava latent
OS   virus (isolate Nigerian)).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Begomovirus.
OX   NCBI_TaxID=222073;
OH   NCBI_TaxID=197394; Hewittia sublobata.
OH   NCBI_TaxID=3996; Jatropha multifida (Coralbush).
OH   NCBI_TaxID=194268; Laportea.
OH   NCBI_TaxID=3983; Manihot esculenta (Cassava) (Jatropha manihot).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2308831; DOI=10.1093/nar/18.1.197;
RA   Morris B., Coates L., Lowe S., Richardson K., Eddy P.;
RT   "Nucleotide sequence of the infectious cloned DNA components of African
RT   cassava mosaic virus (Nigerian strain).";
RL   Nucleic Acids Res. 18:197-198(1990).
CC   -!- FUNCTION: Binds to the genomic viral ssDNA, shuttles it into and out of
CC       the cell nucleus. Begomoviruses use 2 proteins to transport their DNA
CC       from cell to cell. The nuclear shuttle protein (NSP) shuttles it
CC       between nucleus and cytoplasm and the movement protein (MP) probably
CC       transports the DNA-NSP complex to the cell periphery and facilitates
CC       movement across the cell wall (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Binds to single-stranded and double-stranded viral DNA.
CC       Interacts with the host nuclear shuttle interacting (NSI) protein. This
CC       interaction may allow NSP to recruit NSI monomers to the viral genome
CC       and thus regulate nuclear export of viral genome by NSP (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Host cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC       Note=Translocated to the plasma membrane by the movement protein BC1.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the begomovirus nuclear shuttle protein family.
CC       {ECO:0000305}.
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DR   EMBL; X17096; CAA34954.1; -; Genomic_DNA.
DR   PIR; S07595; S07595.
DR   PRIDE; P14970; -.
DR   Proteomes; UP000008453; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0051027; P:DNA transport; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.20; -; 1.
DR   InterPro; IPR001530; Gemini_BR1.
DR   InterPro; IPR000263; GV_A/BR1_coat.
DR   InterPro; IPR029053; Viral_coat.
DR   Pfam; PF00844; Gemini_coat; 1.
DR   PRINTS; PR00225; GEMCOATBR1.
PE   3: Inferred from homology;
KW   DNA-binding; Host cell membrane; Host cytoplasm; Host membrane;
KW   Host nucleus; Host-virus interaction; Membrane; Transport;
KW   Viral movement protein.
FT   CHAIN           1..256
FT                   /note="Nuclear shuttle protein"
FT                   /id="PRO_0000222263"
FT   REGION          150..187
FT                   /note="Interaction with Arabidopsis thaliana NSI protein"
FT                   /evidence="ECO:0000250"
FT   MOTIF           18..39
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           81..96
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   256 AA;  29379 MW;  0C7A3274C97550A8 CRC64;
     MYSIRKQPRN FQRKFNSNTT NRFPIRRKYV GGHTRPSVRR RLTYEPVERP LVHNVLCEKQ
     HGDVFNLQQN TSYTSFVTYP SRGPSGDGRS RDYIKLQSMS VSGVIHAKAN CNDDPMEVSH
     DVNGVFVFSL IMDTKPYLPA GVQALPTFEE LFGAYSACYV NLRLLNNQQH RYRVLHSVKR
     FVSSAGDTKV SQFRFNKRLS TRRYNIWASF HDGDLVNAGG NYRNISKNAI LVSYAFVSEH
     SMSCKPFVQI ETSYVG