ID   NSP_ICMV                Reviewed;         260 AA.
AC   Q08595;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Nuclear shuttle protein;
DE            Short=NSP;
DE   AltName: Full=Protein BR1;
DE   AltName: Full=Protein BV1;
GN   ORFNames=BR1, BV1;
OS   Indian cassava mosaic virus (ICMV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Begomovirus.
OX   NCBI_TaxID=31600;
OH   NCBI_TaxID=3983; Manihot esculenta (Cassava) (Jatropha manihot).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8245859; DOI=10.1099/0022-1317-74-11-2437;
RA   Hong Y.G., Robinson D.J., Harrison B.D.;
RT   "Nucleotide sequence evidence for the occurrence of three distinct
RT   whitefly-transmitted geminiviruses in cassava.";
RL   J. Gen. Virol. 74:2437-2443(1993).
CC   -!- FUNCTION: Binds to the genomic viral ssDNA, shuttles it into and out of
CC       the cell nucleus. Begomoviruses use 2 proteins to transport their DNA
CC       from cell to cell. The nuclear shuttle protein (NSP) shuttles it
CC       between nucleus and cytoplasm and the movement protein (MP) probably
CC       transports the DNA-NSP complex to the cell periphery and facilitates
CC       movement across the cell wall (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Binds to single-stranded and double-stranded viral DNA.
CC       Interacts with the host nuclear shuttle interacting (NSI) protein. This
CC       interaction may allow NSP to recruit NSI monomers to the viral genome
CC       and thus regulate nuclear export of viral genome by NSP (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Host cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC       Note=Translocated to the plasma membrane by the movement protein BC1.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the begomovirus nuclear shuttle protein family.
CC       {ECO:0000305}.
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DR   EMBL; Z24759; CAA80892.1; -; Genomic_DNA.
DR   PIR; JQ2330; JQ2330.
DR   RefSeq; NP_047235.1; NC_001933.1.
DR   PRIDE; Q08595; -.
DR   GeneID; 991062; -.
DR   KEGG; vg:991062; -.
DR   Proteomes; UP000007210; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0051027; P:DNA transport; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.20; -; 1.
DR   InterPro; IPR001530; Gemini_BR1.
DR   InterPro; IPR000263; GV_A/BR1_coat.
DR   InterPro; IPR029053; Viral_coat.
DR   Pfam; PF00844; Gemini_coat; 1.
DR   PRINTS; PR00225; GEMCOATBR1.
PE   3: Inferred from homology;
KW   DNA-binding; Host cell membrane; Host cytoplasm; Host membrane;
KW   Host nucleus; Host-virus interaction; Membrane; Reference proteome;
KW   Transport; Viral movement protein.
FT   CHAIN           1..260
FT                   /note="Nuclear shuttle protein"
FT                   /id="PRO_0000222264"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          154..191
FT                   /note="Interaction with Arabidopsis thaliana NSI protein"
FT                   /evidence="ECO:0000250"
FT   MOTIF           21..42
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           84..100
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   260 AA;  29785 MW;  E5D9A59C6CE378D8 CRC64;
     MRRGAYTPRS TPFPRDRRSY NAGKGRSFRS YRRRGPVRPL ARRNLFGDDH ARAFTYKTLS
     EDQFGPDFTI HNNNYKSSYI SMPVKTRALS DNRVGDYIKL VNISFTGTVC IKNSQMESDG
     SPMLGLHGLF TCVLVRDKTP RIYSATEPLI PFPQLFGSIN ASYADLSIQD PYKDRFTVIR
     QVSYPVNTEK GDHMCRFKGT RRFGGRYPIW TSFKDDGGSG DSSGLYSNTY KNAILVYYVW
     LSDVSSQLEM YCKYVTRYIG