ID   NSP_MYMVV               Reviewed;         256 AA.
AC   Q9YPS8;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   02-JUN-2021, entry version 55.
DE   RecName: Full=Nuclear shuttle protein;
DE            Short=NSP;
DE   AltName: Full=Protein BR1;
DE   AltName: Full=Protein BV1;
GN   ORFNames=BR1, BV1;
OS   Mungbean yellow mosaic virus (strain Vigna) (MYMV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Begomovirus.
OX   NCBI_TaxID=223295;
OH   NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH   NCBI_TaxID=3915; Vigna mungo (Black gram) (Phaseolus mungo).
OH   NCBI_TaxID=157791; Vigna radiata (Mung bean).
OH   NCBI_TaxID=3916; Vigna radiata var. radiata (Mung bean) (Phaseolus aureus).
OH   NCBI_TaxID=3917; Vigna unguiculata (Cowpea).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15290387; DOI=10.1007/s00705-004-0313-z;
RA   Karthikeyan A.S., Vanitharani R., Balaji V., Anuradha S.,
RA   Thillaichidambaram P., Shivaprasad P.V., Parameswari C., Balamani V.,
RA   Saminathan M., Veluthambi K.;
RT   "Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a
RT   highly variable DNA B component.";
RL   Arch. Virol. 149:1643-1652(2004).
CC   -!- FUNCTION: Binds to the genomic viral ssDNA, shuttles it into and out of
CC       the cell nucleus. Begomoviruses use 2 proteins to transport their DNA
CC       from cell to cell. The nuclear shuttle protein (NSP) shuttles it
CC       between nucleus and cytoplasm and the movement protein (MP) probably
CC       transports the DNA-NSP complex to the cell periphery and facilitates
CC       movement across the cell wall (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Binds to single-stranded and double-stranded viral DNA.
CC       Interacts with the host nuclear shuttle interacting (NSI) protein. This
CC       interaction may allow NSP to recruit NSI monomers to the viral genome
CC       and thus regulate nuclear export of viral genome by NSP (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Host cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC       Note=Translocated to the plasma membrane by the movement protein BC1.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the begomovirus nuclear shuttle protein family.
CC       {ECO:0000305}.
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DR   EMBL; AJ132574; CAA10701.1; -; Genomic_DNA.
DR   PRIDE; Q9YPS8; -.
DR   Proteomes; UP000007784; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0051027; P:DNA transport; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR001530; Gemini_BR1.
DR   InterPro; IPR000263; GV_A/BR1_coat.
DR   Pfam; PF00844; Gemini_coat; 1.
DR   PRINTS; PR00225; GEMCOATBR1.
PE   3: Inferred from homology;
KW   DNA-binding; Host cell membrane; Host cytoplasm; Host membrane;
KW   Host nucleus; Host-virus interaction; Membrane; Reference proteome;
KW   Transport; Viral movement protein.
FT   CHAIN           1..256
FT                   /note="Nuclear shuttle protein"
FT                   /id="PRO_0000323699"
FT   REGION          23..44
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          150..187
FT                   /note="Interaction with Arabidopsis thaliana NSI protein"
FT                   /evidence="ECO:0000250"
FT   MOTIF           18..39
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           81..96
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   256 AA;  29192 MW;  49E927D899C35D57 CRC64;
     MFNRNYRTPL KLRHSNFGFR WQPMTPSRGR LRHNKPSASR KLSYDRVERE MRTNSIVEVQ
     HGSHMSLEKN TDVSSFVQYP ARGINGDGRC RDYIKLLKLD VSGVINIKSS NGDQDMEPGD
     KLSGLFILTV LLDKKPYLPE GVNKLPSFAE LFGPYSAAYA NMHLLDSQKP RFKVLGTIKK
     FVNCTPGTLY GPLKLNMPLS RRKCPLWTTF KDPDQGNCGG NYKNISKNAI VLSYAFISMH
     SLIVEPYFQF ELKYVG